In vitro biocompatibility tests of two commercial types of mineral trioxide aggregate

Detalhes bibliográficos
Autor(a) principal: Ribeiro,Daniel Araki
Data de Publicação: 2005
Outros Autores: Matsumoto,Mariza Akemi, Duarte,Marco Antonio Húngaro, Marques,Mariangela Esther Alencar, Salvadori,Daisy Maria Favero
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Oral Research
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242005000300005
Resumo: Recently, regular and white mineral trioxide aggregate (MTA) are being used in Dentistry as retrofilling materials. Genotoxicity and cytotoxicity tests form an important part of cancer research and risk assessment of potential carcinogens. Thus, the goal of this study was to examine the genotoxicity and cytotoxicity of regular and white MTA in vitro by the single cell gel (comet) assay and trypan blue exclusion test, respectively. Mouse lymphoma cells were exposed to two presentation forms of MTA at final concentrations ranging from 1 to 1,000 µg/mL for 3 h at 37°C. The results showed that both compounds tested did not produce genotoxic effects at all concentrations evaluated. Likewise, no statistically significant differences (p > 0.05) were observed in cytotoxicity. Taken together, our results suggest that regular and white MTA are not genotoxins and are not able to interfere in cellular viability as assessed by single cell gel (comet) assay and trypan blue assay, respectively.
id SBPQO-1_2f468b4688f0af0f8fcd8ea9dcea5d97
oai_identifier_str oai:scielo:S1806-83242005000300005
network_acronym_str SBPQO-1
network_name_str Brazilian Oral Research
repository_id_str
spelling In vitro biocompatibility tests of two commercial types of mineral trioxide aggregateMineral trioxide aggregateGenotoxicity testsComet assayTrypan blueMouse lymphoma cellsRecently, regular and white mineral trioxide aggregate (MTA) are being used in Dentistry as retrofilling materials. Genotoxicity and cytotoxicity tests form an important part of cancer research and risk assessment of potential carcinogens. Thus, the goal of this study was to examine the genotoxicity and cytotoxicity of regular and white MTA in vitro by the single cell gel (comet) assay and trypan blue exclusion test, respectively. Mouse lymphoma cells were exposed to two presentation forms of MTA at final concentrations ranging from 1 to 1,000 µg/mL for 3 h at 37°C. The results showed that both compounds tested did not produce genotoxic effects at all concentrations evaluated. Likewise, no statistically significant differences (p > 0.05) were observed in cytotoxicity. Taken together, our results suggest that regular and white MTA are not genotoxins and are not able to interfere in cellular viability as assessed by single cell gel (comet) assay and trypan blue assay, respectively.Sociedade Brasileira de Pesquisa Odontológica - SBPqO2005-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242005000300005Brazilian Oral Research v.19 n.3 2005reponame:Brazilian Oral Researchinstname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO)instacron:SBPQO10.1590/S1806-83242005000300005info:eu-repo/semantics/openAccessRibeiro,Daniel ArakiMatsumoto,Mariza AkemiDuarte,Marco Antonio HúngaroMarques,Mariangela Esther AlencarSalvadori,Daisy Maria Faveroeng2005-11-21T00:00:00Zoai:scielo:S1806-83242005000300005Revistahttps://www.scielo.br/j/bor/https://old.scielo.br/oai/scielo-oai.phppob@edu.usp.br||bor@sbpqo.org.br1807-31071806-8324opendoar:2005-11-21T00:00Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO)false
dc.title.none.fl_str_mv In vitro biocompatibility tests of two commercial types of mineral trioxide aggregate
title In vitro biocompatibility tests of two commercial types of mineral trioxide aggregate
spellingShingle In vitro biocompatibility tests of two commercial types of mineral trioxide aggregate
Ribeiro,Daniel Araki
Mineral trioxide aggregate
Genotoxicity tests
Comet assay
Trypan blue
Mouse lymphoma cells
title_short In vitro biocompatibility tests of two commercial types of mineral trioxide aggregate
title_full In vitro biocompatibility tests of two commercial types of mineral trioxide aggregate
title_fullStr In vitro biocompatibility tests of two commercial types of mineral trioxide aggregate
title_full_unstemmed In vitro biocompatibility tests of two commercial types of mineral trioxide aggregate
title_sort In vitro biocompatibility tests of two commercial types of mineral trioxide aggregate
author Ribeiro,Daniel Araki
author_facet Ribeiro,Daniel Araki
Matsumoto,Mariza Akemi
Duarte,Marco Antonio Húngaro
Marques,Mariangela Esther Alencar
Salvadori,Daisy Maria Favero
author_role author
author2 Matsumoto,Mariza Akemi
Duarte,Marco Antonio Húngaro
Marques,Mariangela Esther Alencar
Salvadori,Daisy Maria Favero
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Ribeiro,Daniel Araki
Matsumoto,Mariza Akemi
Duarte,Marco Antonio Húngaro
Marques,Mariangela Esther Alencar
Salvadori,Daisy Maria Favero
dc.subject.por.fl_str_mv Mineral trioxide aggregate
Genotoxicity tests
Comet assay
Trypan blue
Mouse lymphoma cells
topic Mineral trioxide aggregate
Genotoxicity tests
Comet assay
Trypan blue
Mouse lymphoma cells
description Recently, regular and white mineral trioxide aggregate (MTA) are being used in Dentistry as retrofilling materials. Genotoxicity and cytotoxicity tests form an important part of cancer research and risk assessment of potential carcinogens. Thus, the goal of this study was to examine the genotoxicity and cytotoxicity of regular and white MTA in vitro by the single cell gel (comet) assay and trypan blue exclusion test, respectively. Mouse lymphoma cells were exposed to two presentation forms of MTA at final concentrations ranging from 1 to 1,000 µg/mL for 3 h at 37°C. The results showed that both compounds tested did not produce genotoxic effects at all concentrations evaluated. Likewise, no statistically significant differences (p > 0.05) were observed in cytotoxicity. Taken together, our results suggest that regular and white MTA are not genotoxins and are not able to interfere in cellular viability as assessed by single cell gel (comet) assay and trypan blue assay, respectively.
publishDate 2005
dc.date.none.fl_str_mv 2005-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242005000300005
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242005000300005
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1806-83242005000300005
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Pesquisa Odontológica - SBPqO
publisher.none.fl_str_mv Sociedade Brasileira de Pesquisa Odontológica - SBPqO
dc.source.none.fl_str_mv Brazilian Oral Research v.19 n.3 2005
reponame:Brazilian Oral Research
instname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO)
instacron:SBPQO
instname_str Sociedade Brasileira de Pesquisa Odontológica (SBPqO)
instacron_str SBPQO
institution SBPQO
reponame_str Brazilian Oral Research
collection Brazilian Oral Research
repository.name.fl_str_mv Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO)
repository.mail.fl_str_mv pob@edu.usp.br||bor@sbpqo.org.br
_version_ 1750318320398106624

Registros relacionados