Efficacy of chemomechanical caries removal in reducing cariogenic microbiota: a randomized clinical trial

Detalhes bibliográficos
Autor(a) principal: AMMARI,Michelle Mikhael
Data de Publicação: 2014
Outros Autores: MOLITERNO,Luiz Flávio Martins, HIRATA JÚNIOR,Raphael, SÉLLOS,Mariana Canano, SOVIERO,Vera Mendes, COUTINHO FILHO,Wagner Pereira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Oral Research
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242014000100242
Resumo: The aim of this study was to compare the efficacy of chemochemical methods (Carisolv™ and Papacárie®) versus the manual method (excavators) in reducing the cariogenic microbiota in dentine caries of primary teeth. Forty-six healthy children (5 to 9 years old) having at least one primary tooth with a cavitated dentine carious lesion were included in the study. The teeth presented no clinical or radiographic signs of pulpal involvement. The sample of 74 teeth was randomly divided into three different groups: Papacárie® (n = 25), Carisolv™ (n = 27) and Manual (n = 22). Samples of carious and sound dentine were collected with sterile excavators before and after caries removal in the three groups. The dentine samples were transferred to glass tubes containing a 1mL thioglycollate medium used as a carrier and enriched for microbiological detection of mutans streptococci and Lactobacillus spp, after incubation for 6h at room temperature. The minimum detection value for colony forming units (CFU) was 3.3 x 102 CFU/ml, and the results were converted into scores from 0 to 4. A significant difference was observed in relation to the microbiological scores before and after caries removal for all methods (Wilcoxon test; p < 0.001). The use of chemomechanical methods for caries removal did not improve the reduction of cariogenic microorganisms in dentine caries lesions, in comparison with manual excavation.
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spelling Efficacy of chemomechanical caries removal in reducing cariogenic microbiota: a randomized clinical trialDental CariesPapainMicrobiologyToothDeciduousThe aim of this study was to compare the efficacy of chemochemical methods (Carisolv™ and Papacárie®) versus the manual method (excavators) in reducing the cariogenic microbiota in dentine caries of primary teeth. Forty-six healthy children (5 to 9 years old) having at least one primary tooth with a cavitated dentine carious lesion were included in the study. The teeth presented no clinical or radiographic signs of pulpal involvement. The sample of 74 teeth was randomly divided into three different groups: Papacárie® (n = 25), Carisolv™ (n = 27) and Manual (n = 22). Samples of carious and sound dentine were collected with sterile excavators before and after caries removal in the three groups. The dentine samples were transferred to glass tubes containing a 1mL thioglycollate medium used as a carrier and enriched for microbiological detection of mutans streptococci and Lactobacillus spp, after incubation for 6h at room temperature. The minimum detection value for colony forming units (CFU) was 3.3 x 102 CFU/ml, and the results were converted into scores from 0 to 4. A significant difference was observed in relation to the microbiological scores before and after caries removal for all methods (Wilcoxon test; p < 0.001). The use of chemomechanical methods for caries removal did not improve the reduction of cariogenic microorganisms in dentine caries lesions, in comparison with manual excavation.Sociedade Brasileira de Pesquisa Odontológica - SBPqO2014-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242014000100242Brazilian Oral Research v.28 n.1 2014reponame:Brazilian Oral Researchinstname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO)instacron:SBPQO10.1590/1807-3107BOR-2014.vol28.0031info:eu-repo/semantics/openAccessAMMARI,Michelle MikhaelMOLITERNO,Luiz Flávio MartinsHIRATA JÚNIOR,RaphaelSÉLLOS,Mariana CananoSOVIERO,Vera MendesCOUTINHO FILHO,Wagner Pereiraeng2018-09-17T00:00:00Zoai:scielo:S1806-83242014000100242Revistahttps://www.scielo.br/j/bor/https://old.scielo.br/oai/scielo-oai.phppob@edu.usp.br||bor@sbpqo.org.br1807-31071806-8324opendoar:2018-09-17T00:00Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO)false
dc.title.none.fl_str_mv Efficacy of chemomechanical caries removal in reducing cariogenic microbiota: a randomized clinical trial
title Efficacy of chemomechanical caries removal in reducing cariogenic microbiota: a randomized clinical trial
spellingShingle Efficacy of chemomechanical caries removal in reducing cariogenic microbiota: a randomized clinical trial
AMMARI,Michelle Mikhael
Dental Caries
Papain
Microbiology
Tooth
Deciduous
title_short Efficacy of chemomechanical caries removal in reducing cariogenic microbiota: a randomized clinical trial
title_full Efficacy of chemomechanical caries removal in reducing cariogenic microbiota: a randomized clinical trial
title_fullStr Efficacy of chemomechanical caries removal in reducing cariogenic microbiota: a randomized clinical trial
title_full_unstemmed Efficacy of chemomechanical caries removal in reducing cariogenic microbiota: a randomized clinical trial
title_sort Efficacy of chemomechanical caries removal in reducing cariogenic microbiota: a randomized clinical trial
author AMMARI,Michelle Mikhael
author_facet AMMARI,Michelle Mikhael
MOLITERNO,Luiz Flávio Martins
HIRATA JÚNIOR,Raphael
SÉLLOS,Mariana Canano
SOVIERO,Vera Mendes
COUTINHO FILHO,Wagner Pereira
author_role author
author2 MOLITERNO,Luiz Flávio Martins
HIRATA JÚNIOR,Raphael
SÉLLOS,Mariana Canano
SOVIERO,Vera Mendes
COUTINHO FILHO,Wagner Pereira
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv AMMARI,Michelle Mikhael
MOLITERNO,Luiz Flávio Martins
HIRATA JÚNIOR,Raphael
SÉLLOS,Mariana Canano
SOVIERO,Vera Mendes
COUTINHO FILHO,Wagner Pereira
dc.subject.por.fl_str_mv Dental Caries
Papain
Microbiology
Tooth
Deciduous
topic Dental Caries
Papain
Microbiology
Tooth
Deciduous
description The aim of this study was to compare the efficacy of chemochemical methods (Carisolv™ and Papacárie®) versus the manual method (excavators) in reducing the cariogenic microbiota in dentine caries of primary teeth. Forty-six healthy children (5 to 9 years old) having at least one primary tooth with a cavitated dentine carious lesion were included in the study. The teeth presented no clinical or radiographic signs of pulpal involvement. The sample of 74 teeth was randomly divided into three different groups: Papacárie® (n = 25), Carisolv™ (n = 27) and Manual (n = 22). Samples of carious and sound dentine were collected with sterile excavators before and after caries removal in the three groups. The dentine samples were transferred to glass tubes containing a 1mL thioglycollate medium used as a carrier and enriched for microbiological detection of mutans streptococci and Lactobacillus spp, after incubation for 6h at room temperature. The minimum detection value for colony forming units (CFU) was 3.3 x 102 CFU/ml, and the results were converted into scores from 0 to 4. A significant difference was observed in relation to the microbiological scores before and after caries removal for all methods (Wilcoxon test; p < 0.001). The use of chemomechanical methods for caries removal did not improve the reduction of cariogenic microorganisms in dentine caries lesions, in comparison with manual excavation.
publishDate 2014
dc.date.none.fl_str_mv 2014-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242014000100242
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242014000100242
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1807-3107BOR-2014.vol28.0031
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Pesquisa Odontológica - SBPqO
publisher.none.fl_str_mv Sociedade Brasileira de Pesquisa Odontológica - SBPqO
dc.source.none.fl_str_mv Brazilian Oral Research v.28 n.1 2014
reponame:Brazilian Oral Research
instname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO)
instacron:SBPQO
instname_str Sociedade Brasileira de Pesquisa Odontológica (SBPqO)
instacron_str SBPQO
institution SBPQO
reponame_str Brazilian Oral Research
collection Brazilian Oral Research
repository.name.fl_str_mv Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO)
repository.mail.fl_str_mv pob@edu.usp.br||bor@sbpqo.org.br
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