Immunohistochemical expression of RANKL in oral giant cell lesions is predictive of aggressiveness

Detalhes bibliográficos
Autor(a) principal: MARTINI,Georgia
Data de Publicação: 2018
Outros Autores: CAPELLA,Diogo, RIVERO,Elena Riet Correa, GONDAK,Rogério Oliveira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Oral Research
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242018000100291
Resumo: Abstract The aim of this study was to evaluate the immunohistochemical expression of receptor activator of nuclear factor kappa-B ligand (RANKL) and of osteoprotegerin (OPG), important proteins correlated with osteoclastogenesis, in central giant cell lesions (CGCL) and peripheral giant cell lesions (PGCL) and to compare their expression with the histological and clinical parameters for quantification of multinucleated giant cells (MGC) and their nuclei, lesion size, and recurrences. Twenty cases of each lesion type were selected to quantify the number of MGCs and nuclei/mm2 of connective tissue. The immunoreactivity of RANKL and OPG was expressed as a percentage of the marked area in the stroma. Clinical data were collected from pathoanatomical and medical reports. No statistical differences were found for the number of MGCs (p = 0.24) between PGCL and CGCL, but the number of nuclei within the MGCs was higher in CGCL (p = 0.01). RANKL expression was higher in CGCL than in PGCL (p = 0.04) and all recurrent lesions showed higher RANKL and OPG expressions than nonrecurrent lesions. We report higher RANKL expression and a greater number of nuclei in CGCL, which may explain the difference in clinical behaviour between these lesions and their pathogenesis.
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spelling Immunohistochemical expression of RANKL in oral giant cell lesions is predictive of aggressivenessNF-kappa BOsteoprotegerinImmunohistochemistryAbstract The aim of this study was to evaluate the immunohistochemical expression of receptor activator of nuclear factor kappa-B ligand (RANKL) and of osteoprotegerin (OPG), important proteins correlated with osteoclastogenesis, in central giant cell lesions (CGCL) and peripheral giant cell lesions (PGCL) and to compare their expression with the histological and clinical parameters for quantification of multinucleated giant cells (MGC) and their nuclei, lesion size, and recurrences. Twenty cases of each lesion type were selected to quantify the number of MGCs and nuclei/mm2 of connective tissue. The immunoreactivity of RANKL and OPG was expressed as a percentage of the marked area in the stroma. Clinical data were collected from pathoanatomical and medical reports. No statistical differences were found for the number of MGCs (p = 0.24) between PGCL and CGCL, but the number of nuclei within the MGCs was higher in CGCL (p = 0.01). RANKL expression was higher in CGCL than in PGCL (p = 0.04) and all recurrent lesions showed higher RANKL and OPG expressions than nonrecurrent lesions. We report higher RANKL expression and a greater number of nuclei in CGCL, which may explain the difference in clinical behaviour between these lesions and their pathogenesis.Sociedade Brasileira de Pesquisa Odontológica - SBPqO2018-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242018000100291Brazilian Oral Research v.32 2018reponame:Brazilian Oral Researchinstname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO)instacron:SBPQO10.1590/1807-3107bor-2018.vol32.0115info:eu-repo/semantics/openAccessMARTINI,GeorgiaCAPELLA,DiogoRIVERO,Elena Riet CorreaGONDAK,Rogério Oliveiraeng2018-10-23T00:00:00Zoai:scielo:S1806-83242018000100291Revistahttps://www.scielo.br/j/bor/https://old.scielo.br/oai/scielo-oai.phppob@edu.usp.br||bor@sbpqo.org.br1807-31071806-8324opendoar:2018-10-23T00:00Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO)false
dc.title.none.fl_str_mv Immunohistochemical expression of RANKL in oral giant cell lesions is predictive of aggressiveness
title Immunohistochemical expression of RANKL in oral giant cell lesions is predictive of aggressiveness
spellingShingle Immunohistochemical expression of RANKL in oral giant cell lesions is predictive of aggressiveness
MARTINI,Georgia
NF-kappa B
Osteoprotegerin
Immunohistochemistry
title_short Immunohistochemical expression of RANKL in oral giant cell lesions is predictive of aggressiveness
title_full Immunohistochemical expression of RANKL in oral giant cell lesions is predictive of aggressiveness
title_fullStr Immunohistochemical expression of RANKL in oral giant cell lesions is predictive of aggressiveness
title_full_unstemmed Immunohistochemical expression of RANKL in oral giant cell lesions is predictive of aggressiveness
title_sort Immunohistochemical expression of RANKL in oral giant cell lesions is predictive of aggressiveness
author MARTINI,Georgia
author_facet MARTINI,Georgia
CAPELLA,Diogo
RIVERO,Elena Riet Correa
GONDAK,Rogério Oliveira
author_role author
author2 CAPELLA,Diogo
RIVERO,Elena Riet Correa
GONDAK,Rogério Oliveira
author2_role author
author
author
dc.contributor.author.fl_str_mv MARTINI,Georgia
CAPELLA,Diogo
RIVERO,Elena Riet Correa
GONDAK,Rogério Oliveira
dc.subject.por.fl_str_mv NF-kappa B
Osteoprotegerin
Immunohistochemistry
topic NF-kappa B
Osteoprotegerin
Immunohistochemistry
description Abstract The aim of this study was to evaluate the immunohistochemical expression of receptor activator of nuclear factor kappa-B ligand (RANKL) and of osteoprotegerin (OPG), important proteins correlated with osteoclastogenesis, in central giant cell lesions (CGCL) and peripheral giant cell lesions (PGCL) and to compare their expression with the histological and clinical parameters for quantification of multinucleated giant cells (MGC) and their nuclei, lesion size, and recurrences. Twenty cases of each lesion type were selected to quantify the number of MGCs and nuclei/mm2 of connective tissue. The immunoreactivity of RANKL and OPG was expressed as a percentage of the marked area in the stroma. Clinical data were collected from pathoanatomical and medical reports. No statistical differences were found for the number of MGCs (p = 0.24) between PGCL and CGCL, but the number of nuclei within the MGCs was higher in CGCL (p = 0.01). RANKL expression was higher in CGCL than in PGCL (p = 0.04) and all recurrent lesions showed higher RANKL and OPG expressions than nonrecurrent lesions. We report higher RANKL expression and a greater number of nuclei in CGCL, which may explain the difference in clinical behaviour between these lesions and their pathogenesis.
publishDate 2018
dc.date.none.fl_str_mv 2018-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242018000100291
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242018000100291
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1807-3107bor-2018.vol32.0115
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Pesquisa Odontológica - SBPqO
publisher.none.fl_str_mv Sociedade Brasileira de Pesquisa Odontológica - SBPqO
dc.source.none.fl_str_mv Brazilian Oral Research v.32 2018
reponame:Brazilian Oral Research
instname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO)
instacron:SBPQO
instname_str Sociedade Brasileira de Pesquisa Odontológica (SBPqO)
instacron_str SBPQO
institution SBPQO
reponame_str Brazilian Oral Research
collection Brazilian Oral Research
repository.name.fl_str_mv Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO)
repository.mail.fl_str_mv pob@edu.usp.br||bor@sbpqo.org.br
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