Epstein-Barr virus in oral hairy leukoplakia scrapes: identification by PCR
Autor(a) principal: | |
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Data de Publicação: | 2005 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Oral Research |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242005000400015 |
Resumo: | Oral hairy leukoplakia (OHL) is a lesion associated with a compromised immune system, and its diagnosis is determined by the demonstration of the presence of Epstein-Barr virus (EBV) in lesional tissue. The purpose of this article was to develop a simple technique to help the diagnosis of OHL, using PCR as an alternative technique to evidence EBV in scrapings. DNA samples were obtained by scraping the lateral border of the tongue of 38 adult patients: 29 HIV-positive patients (4 with clinical evidence of OHL; 4 with history of OHL, but without lesion at the moment the samples were collected; and 21 without clinical evidence of OHL), and 9 healthy volunteers for the control group. DNA was extracted from scrapes and amplified by PCR using specific primers for EBV. Of the 29 cases of HIV-positive patients, 22 (75.86%) were positive for EBV: 2 patients with clinical evidence of OHL, 4 patients with history of OHL, but without lesion at the moment the samples were collected, and 16 patients without clinical evidence of OHL. In the control group, samples of 5 (55.56%) healthy volunteers presented amplification for EBV. We concluded that the use of PCR in oral scrapes suggests a high sensitivity but low specificity for the diagnosis of OHL. |
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Brazilian Oral Research |
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Epstein-Barr virus in oral hairy leukoplakia scrapes: identification by PCREpstein-Barr virus infectionsLeukoplakia, hairyPolymerase chain reactionHIVOral hairy leukoplakia (OHL) is a lesion associated with a compromised immune system, and its diagnosis is determined by the demonstration of the presence of Epstein-Barr virus (EBV) in lesional tissue. The purpose of this article was to develop a simple technique to help the diagnosis of OHL, using PCR as an alternative technique to evidence EBV in scrapings. DNA samples were obtained by scraping the lateral border of the tongue of 38 adult patients: 29 HIV-positive patients (4 with clinical evidence of OHL; 4 with history of OHL, but without lesion at the moment the samples were collected; and 21 without clinical evidence of OHL), and 9 healthy volunteers for the control group. DNA was extracted from scrapes and amplified by PCR using specific primers for EBV. Of the 29 cases of HIV-positive patients, 22 (75.86%) were positive for EBV: 2 patients with clinical evidence of OHL, 4 patients with history of OHL, but without lesion at the moment the samples were collected, and 16 patients without clinical evidence of OHL. In the control group, samples of 5 (55.56%) healthy volunteers presented amplification for EBV. We concluded that the use of PCR in oral scrapes suggests a high sensitivity but low specificity for the diagnosis of OHL.Sociedade Brasileira de Pesquisa Odontológica - SBPqO2005-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242005000400015Brazilian Oral Research v.19 n.4 2005reponame:Brazilian Oral Researchinstname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO)instacron:SBPQO10.1590/S1806-83242005000400015info:eu-repo/semantics/openAccessKomatsu,Tatiana LucianelliRivero,Elena Riet CorreaMagalhães,Marina Helena Cury Gallottini deNunes,Fabio Daumaseng2006-02-14T00:00:00Zoai:scielo:S1806-83242005000400015Revistahttps://www.scielo.br/j/bor/https://old.scielo.br/oai/scielo-oai.phppob@edu.usp.br||bor@sbpqo.org.br1807-31071806-8324opendoar:2006-02-14T00:00Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO)false |
dc.title.none.fl_str_mv |
Epstein-Barr virus in oral hairy leukoplakia scrapes: identification by PCR |
title |
Epstein-Barr virus in oral hairy leukoplakia scrapes: identification by PCR |
spellingShingle |
Epstein-Barr virus in oral hairy leukoplakia scrapes: identification by PCR Komatsu,Tatiana Lucianelli Epstein-Barr virus infections Leukoplakia, hairy Polymerase chain reaction HIV |
title_short |
Epstein-Barr virus in oral hairy leukoplakia scrapes: identification by PCR |
title_full |
Epstein-Barr virus in oral hairy leukoplakia scrapes: identification by PCR |
title_fullStr |
Epstein-Barr virus in oral hairy leukoplakia scrapes: identification by PCR |
title_full_unstemmed |
Epstein-Barr virus in oral hairy leukoplakia scrapes: identification by PCR |
title_sort |
Epstein-Barr virus in oral hairy leukoplakia scrapes: identification by PCR |
author |
Komatsu,Tatiana Lucianelli |
author_facet |
Komatsu,Tatiana Lucianelli Rivero,Elena Riet Correa Magalhães,Marina Helena Cury Gallottini de Nunes,Fabio Daumas |
author_role |
author |
author2 |
Rivero,Elena Riet Correa Magalhães,Marina Helena Cury Gallottini de Nunes,Fabio Daumas |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Komatsu,Tatiana Lucianelli Rivero,Elena Riet Correa Magalhães,Marina Helena Cury Gallottini de Nunes,Fabio Daumas |
dc.subject.por.fl_str_mv |
Epstein-Barr virus infections Leukoplakia, hairy Polymerase chain reaction HIV |
topic |
Epstein-Barr virus infections Leukoplakia, hairy Polymerase chain reaction HIV |
description |
Oral hairy leukoplakia (OHL) is a lesion associated with a compromised immune system, and its diagnosis is determined by the demonstration of the presence of Epstein-Barr virus (EBV) in lesional tissue. The purpose of this article was to develop a simple technique to help the diagnosis of OHL, using PCR as an alternative technique to evidence EBV in scrapings. DNA samples were obtained by scraping the lateral border of the tongue of 38 adult patients: 29 HIV-positive patients (4 with clinical evidence of OHL; 4 with history of OHL, but without lesion at the moment the samples were collected; and 21 without clinical evidence of OHL), and 9 healthy volunteers for the control group. DNA was extracted from scrapes and amplified by PCR using specific primers for EBV. Of the 29 cases of HIV-positive patients, 22 (75.86%) were positive for EBV: 2 patients with clinical evidence of OHL, 4 patients with history of OHL, but without lesion at the moment the samples were collected, and 16 patients without clinical evidence of OHL. In the control group, samples of 5 (55.56%) healthy volunteers presented amplification for EBV. We concluded that the use of PCR in oral scrapes suggests a high sensitivity but low specificity for the diagnosis of OHL. |
publishDate |
2005 |
dc.date.none.fl_str_mv |
2005-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242005000400015 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242005000400015 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1806-83242005000400015 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Pesquisa Odontológica - SBPqO |
publisher.none.fl_str_mv |
Sociedade Brasileira de Pesquisa Odontológica - SBPqO |
dc.source.none.fl_str_mv |
Brazilian Oral Research v.19 n.4 2005 reponame:Brazilian Oral Research instname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO) instacron:SBPQO |
instname_str |
Sociedade Brasileira de Pesquisa Odontológica (SBPqO) |
instacron_str |
SBPQO |
institution |
SBPQO |
reponame_str |
Brazilian Oral Research |
collection |
Brazilian Oral Research |
repository.name.fl_str_mv |
Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO) |
repository.mail.fl_str_mv |
pob@edu.usp.br||bor@sbpqo.org.br |
_version_ |
1750318320720019456 |