Nuclear metallothionein in oral squamous cell carcinoma: clinicopathological parameters and patient survival
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Oral Research |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242018000100276 |
Resumo: | Abstract The aim of this study was to identify the immunoexpression of methallothionein in oral squamous cell carcinoma as well as to address the correlation with clinical features, histological grade and patient survival. Samples were collected from 93 patients with tongue squamous cell carcinoma who presented for follow-up. Immunohistochemical expression of methallothionein in all groups was performed. The scoring system has previously been published by Tsurutani in 2005, which is based on intensity and distribution of staining. We used Kappa index to evaluate the degree of observers’ agreement under metallothionein immunostaining and histological grade. Associations between methallothionein expression and clinical parameters (age, gender, smoking, tumor size, lymph node metastasis and disease stage) were examined for statistical significance using the chi-squared test. The overall survival rates were estimated by the Kaplan-Meier method and the relationship between protein expression and survival was compared using the log-rank test (p < 0.05). Our results showed no statistically significant association between methallothionein immunostaining and the selected clinicopathological variables. Immunohistochemistry results showed positive nuclear immunostaining for metallothionein in 62,37% (58/93) and negative for metallothionein 37,63% (35/93). The degree of examiners agreement by Kappa varied from substantial to perfect and both metallothionein immunostaining and histological grade were explored. The present study suggests that positive methallothionein expression found in tongue squamous cell carcinoma may not help to predict survival in the analyzed samples, as well as no relation between the protein and histological grade and clinical features was observed. In conclusion, the present study suggests that metallothionein is not associated with tongue squamous cell carcinoma clinicopathological characteristics and aggressiveness. |
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Brazilian Oral Research |
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Nuclear metallothionein in oral squamous cell carcinoma: clinicopathological parameters and patient survivalMouth NeoplasmsSurvivalImmunohistochemistryAbstract The aim of this study was to identify the immunoexpression of methallothionein in oral squamous cell carcinoma as well as to address the correlation with clinical features, histological grade and patient survival. Samples were collected from 93 patients with tongue squamous cell carcinoma who presented for follow-up. Immunohistochemical expression of methallothionein in all groups was performed. The scoring system has previously been published by Tsurutani in 2005, which is based on intensity and distribution of staining. We used Kappa index to evaluate the degree of observers’ agreement under metallothionein immunostaining and histological grade. Associations between methallothionein expression and clinical parameters (age, gender, smoking, tumor size, lymph node metastasis and disease stage) were examined for statistical significance using the chi-squared test. The overall survival rates were estimated by the Kaplan-Meier method and the relationship between protein expression and survival was compared using the log-rank test (p < 0.05). Our results showed no statistically significant association between methallothionein immunostaining and the selected clinicopathological variables. Immunohistochemistry results showed positive nuclear immunostaining for metallothionein in 62,37% (58/93) and negative for metallothionein 37,63% (35/93). The degree of examiners agreement by Kappa varied from substantial to perfect and both metallothionein immunostaining and histological grade were explored. The present study suggests that positive methallothionein expression found in tongue squamous cell carcinoma may not help to predict survival in the analyzed samples, as well as no relation between the protein and histological grade and clinical features was observed. In conclusion, the present study suggests that metallothionein is not associated with tongue squamous cell carcinoma clinicopathological characteristics and aggressiveness.Sociedade Brasileira de Pesquisa Odontológica - SBPqO2018-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242018000100276Brazilian Oral Research v.32 2018reponame:Brazilian Oral Researchinstname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO)instacron:SBPQO10.1590/1807-3107bor-2018.vol32.0105info:eu-repo/semantics/openAccessBEZERRA,Girlene Maria do RegoPONTES,Flávia Sirotheau CorrêaCONTE NETO,NicolauNASCIMENTO,Liliane Silva doSOUZA,Lucas Lacerda dePINTO JUNIOR,Décio dos SantosPONTES,Hélder Antônio Rebeloeng2018-10-08T00:00:00Zoai:scielo:S1806-83242018000100276Revistahttps://www.scielo.br/j/bor/https://old.scielo.br/oai/scielo-oai.phppob@edu.usp.br||bor@sbpqo.org.br1807-31071806-8324opendoar:2018-10-08T00:00Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO)false |
dc.title.none.fl_str_mv |
Nuclear metallothionein in oral squamous cell carcinoma: clinicopathological parameters and patient survival |
title |
Nuclear metallothionein in oral squamous cell carcinoma: clinicopathological parameters and patient survival |
spellingShingle |
Nuclear metallothionein in oral squamous cell carcinoma: clinicopathological parameters and patient survival BEZERRA,Girlene Maria do Rego Mouth Neoplasms Survival Immunohistochemistry |
title_short |
Nuclear metallothionein in oral squamous cell carcinoma: clinicopathological parameters and patient survival |
title_full |
Nuclear metallothionein in oral squamous cell carcinoma: clinicopathological parameters and patient survival |
title_fullStr |
Nuclear metallothionein in oral squamous cell carcinoma: clinicopathological parameters and patient survival |
title_full_unstemmed |
Nuclear metallothionein in oral squamous cell carcinoma: clinicopathological parameters and patient survival |
title_sort |
Nuclear metallothionein in oral squamous cell carcinoma: clinicopathological parameters and patient survival |
author |
BEZERRA,Girlene Maria do Rego |
author_facet |
BEZERRA,Girlene Maria do Rego PONTES,Flávia Sirotheau Corrêa CONTE NETO,Nicolau NASCIMENTO,Liliane Silva do SOUZA,Lucas Lacerda de PINTO JUNIOR,Décio dos Santos PONTES,Hélder Antônio Rebelo |
author_role |
author |
author2 |
PONTES,Flávia Sirotheau Corrêa CONTE NETO,Nicolau NASCIMENTO,Liliane Silva do SOUZA,Lucas Lacerda de PINTO JUNIOR,Décio dos Santos PONTES,Hélder Antônio Rebelo |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
BEZERRA,Girlene Maria do Rego PONTES,Flávia Sirotheau Corrêa CONTE NETO,Nicolau NASCIMENTO,Liliane Silva do SOUZA,Lucas Lacerda de PINTO JUNIOR,Décio dos Santos PONTES,Hélder Antônio Rebelo |
dc.subject.por.fl_str_mv |
Mouth Neoplasms Survival Immunohistochemistry |
topic |
Mouth Neoplasms Survival Immunohistochemistry |
description |
Abstract The aim of this study was to identify the immunoexpression of methallothionein in oral squamous cell carcinoma as well as to address the correlation with clinical features, histological grade and patient survival. Samples were collected from 93 patients with tongue squamous cell carcinoma who presented for follow-up. Immunohistochemical expression of methallothionein in all groups was performed. The scoring system has previously been published by Tsurutani in 2005, which is based on intensity and distribution of staining. We used Kappa index to evaluate the degree of observers’ agreement under metallothionein immunostaining and histological grade. Associations between methallothionein expression and clinical parameters (age, gender, smoking, tumor size, lymph node metastasis and disease stage) were examined for statistical significance using the chi-squared test. The overall survival rates were estimated by the Kaplan-Meier method and the relationship between protein expression and survival was compared using the log-rank test (p < 0.05). Our results showed no statistically significant association between methallothionein immunostaining and the selected clinicopathological variables. Immunohistochemistry results showed positive nuclear immunostaining for metallothionein in 62,37% (58/93) and negative for metallothionein 37,63% (35/93). The degree of examiners agreement by Kappa varied from substantial to perfect and both metallothionein immunostaining and histological grade were explored. The present study suggests that positive methallothionein expression found in tongue squamous cell carcinoma may not help to predict survival in the analyzed samples, as well as no relation between the protein and histological grade and clinical features was observed. In conclusion, the present study suggests that metallothionein is not associated with tongue squamous cell carcinoma clinicopathological characteristics and aggressiveness. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242018000100276 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242018000100276 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1807-3107bor-2018.vol32.0105 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Pesquisa Odontológica - SBPqO |
publisher.none.fl_str_mv |
Sociedade Brasileira de Pesquisa Odontológica - SBPqO |
dc.source.none.fl_str_mv |
Brazilian Oral Research v.32 2018 reponame:Brazilian Oral Research instname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO) instacron:SBPQO |
instname_str |
Sociedade Brasileira de Pesquisa Odontológica (SBPqO) |
instacron_str |
SBPQO |
institution |
SBPQO |
reponame_str |
Brazilian Oral Research |
collection |
Brazilian Oral Research |
repository.name.fl_str_mv |
Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO) |
repository.mail.fl_str_mv |
pob@edu.usp.br||bor@sbpqo.org.br |
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1750318326107602944 |