S. mutans biofilm model to evaluate antimicrobial substances and enamel demineralization

Detalhes bibliográficos
Autor(a) principal: Ccahuana-Vásquez,Renzo Alberto
Data de Publicação: 2010
Outros Autores: Cury,Jaime Aparecido
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Oral Research
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242010000200002
Resumo: The aim of this study was to validate a model of S. mutans biofilm formation, which simulated 'feast-famine' episodes of exposure to sucrose that occur in the oral cavity, showed dose-response susceptibility to antimicrobials and allowed the evaluation of substances with anticaries potential. S. mutans UA159 biofilms were grown for 5 days on bovine enamel slabs at 37°C, 10% CO2. To validate the model, the biofilms were treated 2x/day with chlorhexidine digluconate (CHX) at 0.012, 0.024 and 0.12% (concentration with recognized anti-plaque effect) and 0.05% NaF (concentration with recognized anti-caries effect). CHX showed dose-response effect decreasing biomass, bacterial viability and enamel demineralization (p < 0.05). Whereas, 0.05% NaF did not show antimicrobial effect but had similar effect to that of 0.12% CHX decreasing enamel demineralization (p < 0.05). The model developed has potential to evaluate the effect of substances on biofilm growth and on enamel demineralization.
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spelling S. mutans biofilm model to evaluate antimicrobial substances and enamel demineralizationStreptococcus mutansBiofilmsChlorhexidineSucroseDental cariesThe aim of this study was to validate a model of S. mutans biofilm formation, which simulated 'feast-famine' episodes of exposure to sucrose that occur in the oral cavity, showed dose-response susceptibility to antimicrobials and allowed the evaluation of substances with anticaries potential. S. mutans UA159 biofilms were grown for 5 days on bovine enamel slabs at 37°C, 10% CO2. To validate the model, the biofilms were treated 2x/day with chlorhexidine digluconate (CHX) at 0.012, 0.024 and 0.12% (concentration with recognized anti-plaque effect) and 0.05% NaF (concentration with recognized anti-caries effect). CHX showed dose-response effect decreasing biomass, bacterial viability and enamel demineralization (p < 0.05). Whereas, 0.05% NaF did not show antimicrobial effect but had similar effect to that of 0.12% CHX decreasing enamel demineralization (p < 0.05). The model developed has potential to evaluate the effect of substances on biofilm growth and on enamel demineralization.Sociedade Brasileira de Pesquisa Odontológica - SBPqO2010-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242010000200002Brazilian Oral Research v.24 n.2 2010reponame:Brazilian Oral Researchinstname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO)instacron:SBPQO10.1590/S1806-83242010000200002info:eu-repo/semantics/openAccessCcahuana-Vásquez,Renzo AlbertoCury,Jaime Aparecidoeng2010-07-13T00:00:00Zoai:scielo:S1806-83242010000200002Revistahttps://www.scielo.br/j/bor/https://old.scielo.br/oai/scielo-oai.phppob@edu.usp.br||bor@sbpqo.org.br1807-31071806-8324opendoar:2010-07-13T00:00Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO)false
dc.title.none.fl_str_mv S. mutans biofilm model to evaluate antimicrobial substances and enamel demineralization
title S. mutans biofilm model to evaluate antimicrobial substances and enamel demineralization
spellingShingle S. mutans biofilm model to evaluate antimicrobial substances and enamel demineralization
Ccahuana-Vásquez,Renzo Alberto
Streptococcus mutans
Biofilms
Chlorhexidine
Sucrose
Dental caries
title_short S. mutans biofilm model to evaluate antimicrobial substances and enamel demineralization
title_full S. mutans biofilm model to evaluate antimicrobial substances and enamel demineralization
title_fullStr S. mutans biofilm model to evaluate antimicrobial substances and enamel demineralization
title_full_unstemmed S. mutans biofilm model to evaluate antimicrobial substances and enamel demineralization
title_sort S. mutans biofilm model to evaluate antimicrobial substances and enamel demineralization
author Ccahuana-Vásquez,Renzo Alberto
author_facet Ccahuana-Vásquez,Renzo Alberto
Cury,Jaime Aparecido
author_role author
author2 Cury,Jaime Aparecido
author2_role author
dc.contributor.author.fl_str_mv Ccahuana-Vásquez,Renzo Alberto
Cury,Jaime Aparecido
dc.subject.por.fl_str_mv Streptococcus mutans
Biofilms
Chlorhexidine
Sucrose
Dental caries
topic Streptococcus mutans
Biofilms
Chlorhexidine
Sucrose
Dental caries
description The aim of this study was to validate a model of S. mutans biofilm formation, which simulated 'feast-famine' episodes of exposure to sucrose that occur in the oral cavity, showed dose-response susceptibility to antimicrobials and allowed the evaluation of substances with anticaries potential. S. mutans UA159 biofilms were grown for 5 days on bovine enamel slabs at 37°C, 10% CO2. To validate the model, the biofilms were treated 2x/day with chlorhexidine digluconate (CHX) at 0.012, 0.024 and 0.12% (concentration with recognized anti-plaque effect) and 0.05% NaF (concentration with recognized anti-caries effect). CHX showed dose-response effect decreasing biomass, bacterial viability and enamel demineralization (p < 0.05). Whereas, 0.05% NaF did not show antimicrobial effect but had similar effect to that of 0.12% CHX decreasing enamel demineralization (p < 0.05). The model developed has potential to evaluate the effect of substances on biofilm growth and on enamel demineralization.
publishDate 2010
dc.date.none.fl_str_mv 2010-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242010000200002
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242010000200002
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1806-83242010000200002
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Pesquisa Odontológica - SBPqO
publisher.none.fl_str_mv Sociedade Brasileira de Pesquisa Odontológica - SBPqO
dc.source.none.fl_str_mv Brazilian Oral Research v.24 n.2 2010
reponame:Brazilian Oral Research
instname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO)
instacron:SBPQO
instname_str Sociedade Brasileira de Pesquisa Odontológica (SBPqO)
instacron_str SBPQO
institution SBPQO
reponame_str Brazilian Oral Research
collection Brazilian Oral Research
repository.name.fl_str_mv Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO)
repository.mail.fl_str_mv pob@edu.usp.br||bor@sbpqo.org.br
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