Susceptibility of planktonic cultures of Streptococcus mutans to photodynamic therapy with a light-emitting diode
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Oral Research |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242010000400007 |
Resumo: | The objective of this study was to evaluate the effect of photodynamic therapy with erythrosine and rose bengal using a light-emitting diode (LED) on planktonic cultures of S. mutans. Ten S. mutans strains, including nine clinical strains and one reference strain (ATCC 35688), were used. Suspensions containing 10(6) cells/mL were prepared for each strain and were tested under different experimental conditions: a) LED irradiation in the presence of rose bengal as a photosensitizer (RB+L+); b) LED irradiation in the presence of erythrosine as a photosensitizer (E+L+); c) LED irradiation only (P-L+); d) treatment with rose bengal only (RB+L-); e) treatment with erythrosine only (E+L-); and f) no LED irradiation or photosensitizer treatment, which served as a control group (P-L-). After treatment, the strains were seeded onto BHI agar for determination of the number of colony-forming units (CFU/mL). The results were submitted to analysis of variance and the Tukey test (p < 0.05). The number of CFU/mL was significantly lower in the groups submitted to photodynamic therapy (RB+L+ and E+L+) compared to control (P-L-), with a reduction of 6.86 log10 in the RB+L+ group and of 5.16 log10 in the E+L+ group. Photodynamic therapy with rose bengal and erythrosine exerted an antimicrobial effect on all S. mutans strains studied. |
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Susceptibility of planktonic cultures of Streptococcus mutans to photodynamic therapy with a light-emitting diodeStreptococcus mutansPhotochemotherapyRose BengalErythrosineThe objective of this study was to evaluate the effect of photodynamic therapy with erythrosine and rose bengal using a light-emitting diode (LED) on planktonic cultures of S. mutans. Ten S. mutans strains, including nine clinical strains and one reference strain (ATCC 35688), were used. Suspensions containing 10(6) cells/mL were prepared for each strain and were tested under different experimental conditions: a) LED irradiation in the presence of rose bengal as a photosensitizer (RB+L+); b) LED irradiation in the presence of erythrosine as a photosensitizer (E+L+); c) LED irradiation only (P-L+); d) treatment with rose bengal only (RB+L-); e) treatment with erythrosine only (E+L-); and f) no LED irradiation or photosensitizer treatment, which served as a control group (P-L-). After treatment, the strains were seeded onto BHI agar for determination of the number of colony-forming units (CFU/mL). The results were submitted to analysis of variance and the Tukey test (p < 0.05). The number of CFU/mL was significantly lower in the groups submitted to photodynamic therapy (RB+L+ and E+L+) compared to control (P-L-), with a reduction of 6.86 log10 in the RB+L+ group and of 5.16 log10 in the E+L+ group. Photodynamic therapy with rose bengal and erythrosine exerted an antimicrobial effect on all S. mutans strains studied.Sociedade Brasileira de Pesquisa Odontológica - SBPqO2010-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242010000400007Brazilian Oral Research v.24 n.4 2010reponame:Brazilian Oral Researchinstname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO)instacron:SBPQO10.1590/S1806-83242010000400007info:eu-repo/semantics/openAccessCosta,Anna Carolina Borges Pereira aChibebe Junior,JoséPereira,Cristiane AparecidaMachado,Ana Karina da SilvaBeltrame Junior,MiltonJunqueira,Juliana CamposJorge,Antonio Olavo Cardosoeng2010-12-16T00:00:00Zoai:scielo:S1806-83242010000400007Revistahttps://www.scielo.br/j/bor/https://old.scielo.br/oai/scielo-oai.phppob@edu.usp.br||bor@sbpqo.org.br1807-31071806-8324opendoar:2010-12-16T00:00Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO)false |
dc.title.none.fl_str_mv |
Susceptibility of planktonic cultures of Streptococcus mutans to photodynamic therapy with a light-emitting diode |
title |
Susceptibility of planktonic cultures of Streptococcus mutans to photodynamic therapy with a light-emitting diode |
spellingShingle |
Susceptibility of planktonic cultures of Streptococcus mutans to photodynamic therapy with a light-emitting diode Costa,Anna Carolina Borges Pereira a Streptococcus mutans Photochemotherapy Rose Bengal Erythrosine |
title_short |
Susceptibility of planktonic cultures of Streptococcus mutans to photodynamic therapy with a light-emitting diode |
title_full |
Susceptibility of planktonic cultures of Streptococcus mutans to photodynamic therapy with a light-emitting diode |
title_fullStr |
Susceptibility of planktonic cultures of Streptococcus mutans to photodynamic therapy with a light-emitting diode |
title_full_unstemmed |
Susceptibility of planktonic cultures of Streptococcus mutans to photodynamic therapy with a light-emitting diode |
title_sort |
Susceptibility of planktonic cultures of Streptococcus mutans to photodynamic therapy with a light-emitting diode |
author |
Costa,Anna Carolina Borges Pereira a |
author_facet |
Costa,Anna Carolina Borges Pereira a Chibebe Junior,José Pereira,Cristiane Aparecida Machado,Ana Karina da Silva Beltrame Junior,Milton Junqueira,Juliana Campos Jorge,Antonio Olavo Cardoso |
author_role |
author |
author2 |
Chibebe Junior,José Pereira,Cristiane Aparecida Machado,Ana Karina da Silva Beltrame Junior,Milton Junqueira,Juliana Campos Jorge,Antonio Olavo Cardoso |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Costa,Anna Carolina Borges Pereira a Chibebe Junior,José Pereira,Cristiane Aparecida Machado,Ana Karina da Silva Beltrame Junior,Milton Junqueira,Juliana Campos Jorge,Antonio Olavo Cardoso |
dc.subject.por.fl_str_mv |
Streptococcus mutans Photochemotherapy Rose Bengal Erythrosine |
topic |
Streptococcus mutans Photochemotherapy Rose Bengal Erythrosine |
description |
The objective of this study was to evaluate the effect of photodynamic therapy with erythrosine and rose bengal using a light-emitting diode (LED) on planktonic cultures of S. mutans. Ten S. mutans strains, including nine clinical strains and one reference strain (ATCC 35688), were used. Suspensions containing 10(6) cells/mL were prepared for each strain and were tested under different experimental conditions: a) LED irradiation in the presence of rose bengal as a photosensitizer (RB+L+); b) LED irradiation in the presence of erythrosine as a photosensitizer (E+L+); c) LED irradiation only (P-L+); d) treatment with rose bengal only (RB+L-); e) treatment with erythrosine only (E+L-); and f) no LED irradiation or photosensitizer treatment, which served as a control group (P-L-). After treatment, the strains were seeded onto BHI agar for determination of the number of colony-forming units (CFU/mL). The results were submitted to analysis of variance and the Tukey test (p < 0.05). The number of CFU/mL was significantly lower in the groups submitted to photodynamic therapy (RB+L+ and E+L+) compared to control (P-L-), with a reduction of 6.86 log10 in the RB+L+ group and of 5.16 log10 in the E+L+ group. Photodynamic therapy with rose bengal and erythrosine exerted an antimicrobial effect on all S. mutans strains studied. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242010000400007 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242010000400007 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1806-83242010000400007 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Pesquisa Odontológica - SBPqO |
publisher.none.fl_str_mv |
Sociedade Brasileira de Pesquisa Odontológica - SBPqO |
dc.source.none.fl_str_mv |
Brazilian Oral Research v.24 n.4 2010 reponame:Brazilian Oral Research instname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO) instacron:SBPQO |
instname_str |
Sociedade Brasileira de Pesquisa Odontológica (SBPqO) |
instacron_str |
SBPQO |
institution |
SBPQO |
reponame_str |
Brazilian Oral Research |
collection |
Brazilian Oral Research |
repository.name.fl_str_mv |
Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO) |
repository.mail.fl_str_mv |
pob@edu.usp.br||bor@sbpqo.org.br |
_version_ |
1750318322090508288 |