Immunohistochemical analysis of FoxP3+ regulatory T cells in lower lip squamous cell carcinomas

Detalhes bibliográficos
Autor(a) principal: da CUNHA FILHO,Fernando Antonio Portela
Data de Publicação: 2016
Outros Autores: AGUIAR,Maria Cássia Ferreira de, SOUZA,Lélia Batista de, PINTO,Leão PEREIRA, GODOY,Gustavo Pina, ALVES,Pollianna Muniz, NONAKA,Cassiano Francisco Weege
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Oral Research
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242016000100321
Resumo: Abstract: The aim of this study was to determine the number of FoxP3+ regulatory T (Treg) cells in the microenvironment of lower lip squamous cell carcinomas (LLSCCs) and to correlate the findings with clinicopathological parameters (tumor size/extent, regional lymph node metastasis, clinical stage, and histopathological grade of malignancy). Fifty cases of LLSCC were selected. Lymphocytes exhibiting nuclear immunostaining for FoxP3 were quantified in 10 microscopic fields at the deep invasive front of LLSCCs. The results were analyzed statistically using the nonparametric Mann-Whitney test and Fisher's exact test. FoxP3+ lymphocytes were observed in all cases studied. The number of these cells tended to be higher in smaller tumors, tumors without regional lymph node metastasis, and tumors in early clinical stages, but the difference was not statistically significant (p > 0.05). Low-grade tumors contained a larger number of FoxP3+ lymphocytes than high-grade tumors (p = 0.019). Tumors with an intense inflammatory infiltrate exhibited a larger number of Treg cells (p = 0.035). On the other hand, the number of FoxP3+ lymphocytes was smaller in tumors arranged in small cell clusters (p = 0.003). No significant differences in the number of FoxP3+ lymphocytes were observed according to the degree of keratinization (p = 0.525) or nuclear pleomorphism (p = 0.343). The results suggest the participation of Treg cells in immune and inflammatory responses in the microenvironment of LLSCCs. These cells may play a more important role in early stages rather than in advanced stages of lip carcinogenesis.
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spelling Immunohistochemical analysis of FoxP3+ regulatory T cells in lower lip squamous cell carcinomasCarcinoma, Squamous CellLipT-Lymphocytes, RegulatoryImmunohistochemistryAbstract: The aim of this study was to determine the number of FoxP3+ regulatory T (Treg) cells in the microenvironment of lower lip squamous cell carcinomas (LLSCCs) and to correlate the findings with clinicopathological parameters (tumor size/extent, regional lymph node metastasis, clinical stage, and histopathological grade of malignancy). Fifty cases of LLSCC were selected. Lymphocytes exhibiting nuclear immunostaining for FoxP3 were quantified in 10 microscopic fields at the deep invasive front of LLSCCs. The results were analyzed statistically using the nonparametric Mann-Whitney test and Fisher's exact test. FoxP3+ lymphocytes were observed in all cases studied. The number of these cells tended to be higher in smaller tumors, tumors without regional lymph node metastasis, and tumors in early clinical stages, but the difference was not statistically significant (p > 0.05). Low-grade tumors contained a larger number of FoxP3+ lymphocytes than high-grade tumors (p = 0.019). Tumors with an intense inflammatory infiltrate exhibited a larger number of Treg cells (p = 0.035). On the other hand, the number of FoxP3+ lymphocytes was smaller in tumors arranged in small cell clusters (p = 0.003). No significant differences in the number of FoxP3+ lymphocytes were observed according to the degree of keratinization (p = 0.525) or nuclear pleomorphism (p = 0.343). The results suggest the participation of Treg cells in immune and inflammatory responses in the microenvironment of LLSCCs. These cells may play a more important role in early stages rather than in advanced stages of lip carcinogenesis.Sociedade Brasileira de Pesquisa Odontológica - SBPqO2016-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242016000100321Brazilian Oral Research v.30 n.1 2016reponame:Brazilian Oral Researchinstname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO)instacron:SBPQO10.1590/1807-3107bor-2016.vol30.0130info:eu-repo/semantics/openAccessda CUNHA FILHO,Fernando Antonio PortelaAGUIAR,Maria Cássia Ferreira deSOUZA,Lélia Batista dePINTO,Leão PEREIRAGODOY,Gustavo PinaALVES,Pollianna MunizNONAKA,Cassiano Francisco Weegeeng2016-12-19T00:00:00Zoai:scielo:S1806-83242016000100321Revistahttps://www.scielo.br/j/bor/https://old.scielo.br/oai/scielo-oai.phppob@edu.usp.br||bor@sbpqo.org.br1807-31071806-8324opendoar:2016-12-19T00:00Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO)false
dc.title.none.fl_str_mv Immunohistochemical analysis of FoxP3+ regulatory T cells in lower lip squamous cell carcinomas
title Immunohistochemical analysis of FoxP3+ regulatory T cells in lower lip squamous cell carcinomas
spellingShingle Immunohistochemical analysis of FoxP3+ regulatory T cells in lower lip squamous cell carcinomas
da CUNHA FILHO,Fernando Antonio Portela
Carcinoma, Squamous Cell
Lip
T-Lymphocytes, Regulatory
Immunohistochemistry
title_short Immunohistochemical analysis of FoxP3+ regulatory T cells in lower lip squamous cell carcinomas
title_full Immunohistochemical analysis of FoxP3+ regulatory T cells in lower lip squamous cell carcinomas
title_fullStr Immunohistochemical analysis of FoxP3+ regulatory T cells in lower lip squamous cell carcinomas
title_full_unstemmed Immunohistochemical analysis of FoxP3+ regulatory T cells in lower lip squamous cell carcinomas
title_sort Immunohistochemical analysis of FoxP3+ regulatory T cells in lower lip squamous cell carcinomas
author da CUNHA FILHO,Fernando Antonio Portela
author_facet da CUNHA FILHO,Fernando Antonio Portela
AGUIAR,Maria Cássia Ferreira de
SOUZA,Lélia Batista de
PINTO,Leão PEREIRA
GODOY,Gustavo Pina
ALVES,Pollianna Muniz
NONAKA,Cassiano Francisco Weege
author_role author
author2 AGUIAR,Maria Cássia Ferreira de
SOUZA,Lélia Batista de
PINTO,Leão PEREIRA
GODOY,Gustavo Pina
ALVES,Pollianna Muniz
NONAKA,Cassiano Francisco Weege
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv da CUNHA FILHO,Fernando Antonio Portela
AGUIAR,Maria Cássia Ferreira de
SOUZA,Lélia Batista de
PINTO,Leão PEREIRA
GODOY,Gustavo Pina
ALVES,Pollianna Muniz
NONAKA,Cassiano Francisco Weege
dc.subject.por.fl_str_mv Carcinoma, Squamous Cell
Lip
T-Lymphocytes, Regulatory
Immunohistochemistry
topic Carcinoma, Squamous Cell
Lip
T-Lymphocytes, Regulatory
Immunohistochemistry
description Abstract: The aim of this study was to determine the number of FoxP3+ regulatory T (Treg) cells in the microenvironment of lower lip squamous cell carcinomas (LLSCCs) and to correlate the findings with clinicopathological parameters (tumor size/extent, regional lymph node metastasis, clinical stage, and histopathological grade of malignancy). Fifty cases of LLSCC were selected. Lymphocytes exhibiting nuclear immunostaining for FoxP3 were quantified in 10 microscopic fields at the deep invasive front of LLSCCs. The results were analyzed statistically using the nonparametric Mann-Whitney test and Fisher's exact test. FoxP3+ lymphocytes were observed in all cases studied. The number of these cells tended to be higher in smaller tumors, tumors without regional lymph node metastasis, and tumors in early clinical stages, but the difference was not statistically significant (p > 0.05). Low-grade tumors contained a larger number of FoxP3+ lymphocytes than high-grade tumors (p = 0.019). Tumors with an intense inflammatory infiltrate exhibited a larger number of Treg cells (p = 0.035). On the other hand, the number of FoxP3+ lymphocytes was smaller in tumors arranged in small cell clusters (p = 0.003). No significant differences in the number of FoxP3+ lymphocytes were observed according to the degree of keratinization (p = 0.525) or nuclear pleomorphism (p = 0.343). The results suggest the participation of Treg cells in immune and inflammatory responses in the microenvironment of LLSCCs. These cells may play a more important role in early stages rather than in advanced stages of lip carcinogenesis.
publishDate 2016
dc.date.none.fl_str_mv 2016-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242016000100321
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242016000100321
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1807-3107bor-2016.vol30.0130
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Pesquisa Odontológica - SBPqO
publisher.none.fl_str_mv Sociedade Brasileira de Pesquisa Odontológica - SBPqO
dc.source.none.fl_str_mv Brazilian Oral Research v.30 n.1 2016
reponame:Brazilian Oral Research
instname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO)
instacron:SBPQO
instname_str Sociedade Brasileira de Pesquisa Odontológica (SBPqO)
instacron_str SBPQO
institution SBPQO
reponame_str Brazilian Oral Research
collection Brazilian Oral Research
repository.name.fl_str_mv Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO)
repository.mail.fl_str_mv pob@edu.usp.br||bor@sbpqo.org.br
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