Immunohistochemical analysis of FoxP3+ regulatory T cells in lower lip squamous cell carcinomas
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Oral Research |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242016000100321 |
Resumo: | Abstract: The aim of this study was to determine the number of FoxP3+ regulatory T (Treg) cells in the microenvironment of lower lip squamous cell carcinomas (LLSCCs) and to correlate the findings with clinicopathological parameters (tumor size/extent, regional lymph node metastasis, clinical stage, and histopathological grade of malignancy). Fifty cases of LLSCC were selected. Lymphocytes exhibiting nuclear immunostaining for FoxP3 were quantified in 10 microscopic fields at the deep invasive front of LLSCCs. The results were analyzed statistically using the nonparametric Mann-Whitney test and Fisher's exact test. FoxP3+ lymphocytes were observed in all cases studied. The number of these cells tended to be higher in smaller tumors, tumors without regional lymph node metastasis, and tumors in early clinical stages, but the difference was not statistically significant (p > 0.05). Low-grade tumors contained a larger number of FoxP3+ lymphocytes than high-grade tumors (p = 0.019). Tumors with an intense inflammatory infiltrate exhibited a larger number of Treg cells (p = 0.035). On the other hand, the number of FoxP3+ lymphocytes was smaller in tumors arranged in small cell clusters (p = 0.003). No significant differences in the number of FoxP3+ lymphocytes were observed according to the degree of keratinization (p = 0.525) or nuclear pleomorphism (p = 0.343). The results suggest the participation of Treg cells in immune and inflammatory responses in the microenvironment of LLSCCs. These cells may play a more important role in early stages rather than in advanced stages of lip carcinogenesis. |
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Immunohistochemical analysis of FoxP3+ regulatory T cells in lower lip squamous cell carcinomasCarcinoma, Squamous CellLipT-Lymphocytes, RegulatoryImmunohistochemistryAbstract: The aim of this study was to determine the number of FoxP3+ regulatory T (Treg) cells in the microenvironment of lower lip squamous cell carcinomas (LLSCCs) and to correlate the findings with clinicopathological parameters (tumor size/extent, regional lymph node metastasis, clinical stage, and histopathological grade of malignancy). Fifty cases of LLSCC were selected. Lymphocytes exhibiting nuclear immunostaining for FoxP3 were quantified in 10 microscopic fields at the deep invasive front of LLSCCs. The results were analyzed statistically using the nonparametric Mann-Whitney test and Fisher's exact test. FoxP3+ lymphocytes were observed in all cases studied. The number of these cells tended to be higher in smaller tumors, tumors without regional lymph node metastasis, and tumors in early clinical stages, but the difference was not statistically significant (p > 0.05). Low-grade tumors contained a larger number of FoxP3+ lymphocytes than high-grade tumors (p = 0.019). Tumors with an intense inflammatory infiltrate exhibited a larger number of Treg cells (p = 0.035). On the other hand, the number of FoxP3+ lymphocytes was smaller in tumors arranged in small cell clusters (p = 0.003). No significant differences in the number of FoxP3+ lymphocytes were observed according to the degree of keratinization (p = 0.525) or nuclear pleomorphism (p = 0.343). The results suggest the participation of Treg cells in immune and inflammatory responses in the microenvironment of LLSCCs. These cells may play a more important role in early stages rather than in advanced stages of lip carcinogenesis.Sociedade Brasileira de Pesquisa Odontológica - SBPqO2016-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242016000100321Brazilian Oral Research v.30 n.1 2016reponame:Brazilian Oral Researchinstname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO)instacron:SBPQO10.1590/1807-3107bor-2016.vol30.0130info:eu-repo/semantics/openAccessda CUNHA FILHO,Fernando Antonio PortelaAGUIAR,Maria Cássia Ferreira deSOUZA,Lélia Batista dePINTO,Leão PEREIRAGODOY,Gustavo PinaALVES,Pollianna MunizNONAKA,Cassiano Francisco Weegeeng2016-12-19T00:00:00Zoai:scielo:S1806-83242016000100321Revistahttps://www.scielo.br/j/bor/https://old.scielo.br/oai/scielo-oai.phppob@edu.usp.br||bor@sbpqo.org.br1807-31071806-8324opendoar:2016-12-19T00:00Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO)false |
dc.title.none.fl_str_mv |
Immunohistochemical analysis of FoxP3+ regulatory T cells in lower lip squamous cell carcinomas |
title |
Immunohistochemical analysis of FoxP3+ regulatory T cells in lower lip squamous cell carcinomas |
spellingShingle |
Immunohistochemical analysis of FoxP3+ regulatory T cells in lower lip squamous cell carcinomas da CUNHA FILHO,Fernando Antonio Portela Carcinoma, Squamous Cell Lip T-Lymphocytes, Regulatory Immunohistochemistry |
title_short |
Immunohistochemical analysis of FoxP3+ regulatory T cells in lower lip squamous cell carcinomas |
title_full |
Immunohistochemical analysis of FoxP3+ regulatory T cells in lower lip squamous cell carcinomas |
title_fullStr |
Immunohistochemical analysis of FoxP3+ regulatory T cells in lower lip squamous cell carcinomas |
title_full_unstemmed |
Immunohistochemical analysis of FoxP3+ regulatory T cells in lower lip squamous cell carcinomas |
title_sort |
Immunohistochemical analysis of FoxP3+ regulatory T cells in lower lip squamous cell carcinomas |
author |
da CUNHA FILHO,Fernando Antonio Portela |
author_facet |
da CUNHA FILHO,Fernando Antonio Portela AGUIAR,Maria Cássia Ferreira de SOUZA,Lélia Batista de PINTO,Leão PEREIRA GODOY,Gustavo Pina ALVES,Pollianna Muniz NONAKA,Cassiano Francisco Weege |
author_role |
author |
author2 |
AGUIAR,Maria Cássia Ferreira de SOUZA,Lélia Batista de PINTO,Leão PEREIRA GODOY,Gustavo Pina ALVES,Pollianna Muniz NONAKA,Cassiano Francisco Weege |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
da CUNHA FILHO,Fernando Antonio Portela AGUIAR,Maria Cássia Ferreira de SOUZA,Lélia Batista de PINTO,Leão PEREIRA GODOY,Gustavo Pina ALVES,Pollianna Muniz NONAKA,Cassiano Francisco Weege |
dc.subject.por.fl_str_mv |
Carcinoma, Squamous Cell Lip T-Lymphocytes, Regulatory Immunohistochemistry |
topic |
Carcinoma, Squamous Cell Lip T-Lymphocytes, Regulatory Immunohistochemistry |
description |
Abstract: The aim of this study was to determine the number of FoxP3+ regulatory T (Treg) cells in the microenvironment of lower lip squamous cell carcinomas (LLSCCs) and to correlate the findings with clinicopathological parameters (tumor size/extent, regional lymph node metastasis, clinical stage, and histopathological grade of malignancy). Fifty cases of LLSCC were selected. Lymphocytes exhibiting nuclear immunostaining for FoxP3 were quantified in 10 microscopic fields at the deep invasive front of LLSCCs. The results were analyzed statistically using the nonparametric Mann-Whitney test and Fisher's exact test. FoxP3+ lymphocytes were observed in all cases studied. The number of these cells tended to be higher in smaller tumors, tumors without regional lymph node metastasis, and tumors in early clinical stages, but the difference was not statistically significant (p > 0.05). Low-grade tumors contained a larger number of FoxP3+ lymphocytes than high-grade tumors (p = 0.019). Tumors with an intense inflammatory infiltrate exhibited a larger number of Treg cells (p = 0.035). On the other hand, the number of FoxP3+ lymphocytes was smaller in tumors arranged in small cell clusters (p = 0.003). No significant differences in the number of FoxP3+ lymphocytes were observed according to the degree of keratinization (p = 0.525) or nuclear pleomorphism (p = 0.343). The results suggest the participation of Treg cells in immune and inflammatory responses in the microenvironment of LLSCCs. These cells may play a more important role in early stages rather than in advanced stages of lip carcinogenesis. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242016000100321 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242016000100321 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1807-3107bor-2016.vol30.0130 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Pesquisa Odontológica - SBPqO |
publisher.none.fl_str_mv |
Sociedade Brasileira de Pesquisa Odontológica - SBPqO |
dc.source.none.fl_str_mv |
Brazilian Oral Research v.30 n.1 2016 reponame:Brazilian Oral Research instname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO) instacron:SBPQO |
instname_str |
Sociedade Brasileira de Pesquisa Odontológica (SBPqO) |
instacron_str |
SBPQO |
institution |
SBPQO |
reponame_str |
Brazilian Oral Research |
collection |
Brazilian Oral Research |
repository.name.fl_str_mv |
Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO) |
repository.mail.fl_str_mv |
pob@edu.usp.br||bor@sbpqo.org.br |
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1750318325180661760 |