Detection and enumeration of periodontopathogenic bacteria in subgingival biofilm of pregnant women
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Oral Research |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242012000500011 |
Resumo: | The aim of this study was to use the fluorescence in situ hybridization (FISH) technique to test the hypothesis of qualitative and quantitative differences of 8 periodontopathogens between pregnant and non-pregnant women. This cross-sectional study included 20 pregnant women in their second trimester of pregnancy and 20 non-pregnant women. Probing depth, bleeding on probing, clinical attachment level, and presence of calculus were recorded. Subgingival plaque samples were collected and the FISH technique identified the presence and numbers of Aggregatibacter actinomycetemcomitans, Tannerella forsythia, Campylobacter rectus, Porphyromonas gingivalis, Treponema denticola, Fusobacterium nucleatum, Prevotella intermedia and Prevotella nigrescens. The Mann-Whitney U-test was applied to compare the data between the two groups. The mean age, ethnicity, marital status, education, and economic level in both groups were similar. The clinical parameters showed no significant differences between pregnant and non-pregnant women. The numbers of subgingival periodontopathogens were not found to be significantly different between groups, despite the higher mean counts of P. intermedia in pregnant women. Colonization patterns of the different bacteria most commonly associated with periodontal disease were not different in the subgingival plaque of pregnant and non-pregnant women. |
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Detection and enumeration of periodontopathogenic bacteria in subgingival biofilm of pregnant womenPregnancyMicrobiologyBiofilmsIn situ HybridizationFluorescenceThe aim of this study was to use the fluorescence in situ hybridization (FISH) technique to test the hypothesis of qualitative and quantitative differences of 8 periodontopathogens between pregnant and non-pregnant women. This cross-sectional study included 20 pregnant women in their second trimester of pregnancy and 20 non-pregnant women. Probing depth, bleeding on probing, clinical attachment level, and presence of calculus were recorded. Subgingival plaque samples were collected and the FISH technique identified the presence and numbers of Aggregatibacter actinomycetemcomitans, Tannerella forsythia, Campylobacter rectus, Porphyromonas gingivalis, Treponema denticola, Fusobacterium nucleatum, Prevotella intermedia and Prevotella nigrescens. The Mann-Whitney U-test was applied to compare the data between the two groups. The mean age, ethnicity, marital status, education, and economic level in both groups were similar. The clinical parameters showed no significant differences between pregnant and non-pregnant women. The numbers of subgingival periodontopathogens were not found to be significantly different between groups, despite the higher mean counts of P. intermedia in pregnant women. Colonization patterns of the different bacteria most commonly associated with periodontal disease were not different in the subgingival plaque of pregnant and non-pregnant women.Sociedade Brasileira de Pesquisa Odontológica - SBPqO2012-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242012000500011Brazilian Oral Research v.26 n.5 2012reponame:Brazilian Oral Researchinstname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO)instacron:SBPQO10.1590/S1806-83242012000500011info:eu-repo/semantics/openAccessMachado,Fernanda CamposCesar,Dionéia EvangelistaAssis,Amanda Vervloet Dutra AgostinhoDiniz,Cláudio GaluppoRibeiro,Rosangela Almeidaeng2012-09-21T00:00:00Zoai:scielo:S1806-83242012000500011Revistahttps://www.scielo.br/j/bor/https://old.scielo.br/oai/scielo-oai.phppob@edu.usp.br||bor@sbpqo.org.br1807-31071806-8324opendoar:2012-09-21T00:00Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO)false |
dc.title.none.fl_str_mv |
Detection and enumeration of periodontopathogenic bacteria in subgingival biofilm of pregnant women |
title |
Detection and enumeration of periodontopathogenic bacteria in subgingival biofilm of pregnant women |
spellingShingle |
Detection and enumeration of periodontopathogenic bacteria in subgingival biofilm of pregnant women Machado,Fernanda Campos Pregnancy Microbiology Biofilms In situ Hybridization Fluorescence |
title_short |
Detection and enumeration of periodontopathogenic bacteria in subgingival biofilm of pregnant women |
title_full |
Detection and enumeration of periodontopathogenic bacteria in subgingival biofilm of pregnant women |
title_fullStr |
Detection and enumeration of periodontopathogenic bacteria in subgingival biofilm of pregnant women |
title_full_unstemmed |
Detection and enumeration of periodontopathogenic bacteria in subgingival biofilm of pregnant women |
title_sort |
Detection and enumeration of periodontopathogenic bacteria in subgingival biofilm of pregnant women |
author |
Machado,Fernanda Campos |
author_facet |
Machado,Fernanda Campos Cesar,Dionéia Evangelista Assis,Amanda Vervloet Dutra Agostinho Diniz,Cláudio Galuppo Ribeiro,Rosangela Almeida |
author_role |
author |
author2 |
Cesar,Dionéia Evangelista Assis,Amanda Vervloet Dutra Agostinho Diniz,Cláudio Galuppo Ribeiro,Rosangela Almeida |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Machado,Fernanda Campos Cesar,Dionéia Evangelista Assis,Amanda Vervloet Dutra Agostinho Diniz,Cláudio Galuppo Ribeiro,Rosangela Almeida |
dc.subject.por.fl_str_mv |
Pregnancy Microbiology Biofilms In situ Hybridization Fluorescence |
topic |
Pregnancy Microbiology Biofilms In situ Hybridization Fluorescence |
description |
The aim of this study was to use the fluorescence in situ hybridization (FISH) technique to test the hypothesis of qualitative and quantitative differences of 8 periodontopathogens between pregnant and non-pregnant women. This cross-sectional study included 20 pregnant women in their second trimester of pregnancy and 20 non-pregnant women. Probing depth, bleeding on probing, clinical attachment level, and presence of calculus were recorded. Subgingival plaque samples were collected and the FISH technique identified the presence and numbers of Aggregatibacter actinomycetemcomitans, Tannerella forsythia, Campylobacter rectus, Porphyromonas gingivalis, Treponema denticola, Fusobacterium nucleatum, Prevotella intermedia and Prevotella nigrescens. The Mann-Whitney U-test was applied to compare the data between the two groups. The mean age, ethnicity, marital status, education, and economic level in both groups were similar. The clinical parameters showed no significant differences between pregnant and non-pregnant women. The numbers of subgingival periodontopathogens were not found to be significantly different between groups, despite the higher mean counts of P. intermedia in pregnant women. Colonization patterns of the different bacteria most commonly associated with periodontal disease were not different in the subgingival plaque of pregnant and non-pregnant women. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242012000500011 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242012000500011 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1806-83242012000500011 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Pesquisa Odontológica - SBPqO |
publisher.none.fl_str_mv |
Sociedade Brasileira de Pesquisa Odontológica - SBPqO |
dc.source.none.fl_str_mv |
Brazilian Oral Research v.26 n.5 2012 reponame:Brazilian Oral Research instname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO) instacron:SBPQO |
instname_str |
Sociedade Brasileira de Pesquisa Odontológica (SBPqO) |
instacron_str |
SBPQO |
institution |
SBPQO |
reponame_str |
Brazilian Oral Research |
collection |
Brazilian Oral Research |
repository.name.fl_str_mv |
Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO) |
repository.mail.fl_str_mv |
pob@edu.usp.br||bor@sbpqo.org.br |
_version_ |
1750318322925174784 |