Effects of stress hormones on the production of volatile sulfur compounds by periodontopathogenic bacteria
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Oral Research |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242014000100228 |
Resumo: | Little is known about the effects of stress hormones on the etiologic agents of halitosis. Thus, the aim of this study was to evaluate in vitro the effects of adrenaline (ADR), noradrenaline (NA) and cortisol (CORT) on bacteria that produce volatile sulfur compounds (VSC), the major gases responsible for bad breath. Cultures of Fusobacterium nucleatum (Fn), Porphyromonas endodontalis (Pe), Prevotella intermedia (Pi) and Porphyromonas gingivalis (Pg) were exposed to 50 µM ADR, NA and CORT or equivalent volumes of sterile water as controls for 12 and 24 h. Growth was evaluated based on absorbance at 660 nm. Portable gas chromatography was used to measure VSC concentrations. Kruskal-Wallis and the Dunn post-hoc test were used to compare the groups. For Fn, ADR, NA and CORT significantly reduced bacterial growth after 12 h and 24 h (p < 0.05). All the substances tested increased hydrogen sulfide (H2S) production (p < 0.05). For Pe, all the substances tested reduced bacterial development after 24 h (p < 0.05), and NA significantly increased the H2S concentration after 12 h (p < 0.05). In the Pg and Pi cultures, no effects on bacterial growth were observed (p > 0.05). In the Pi cultures, ADR, NA and CORT increased H2S (p < 0.05). Catecholamines and cortisol can interfere with growth and H2S production of sub-gingival species in vitro. This process appears to be complex and supports the association between stress and the production of VSC. |
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Effects of stress hormones on the production of volatile sulfur compounds by periodontopathogenic bacteriaHalitosisCatecholaminesHydrocortisoneGram-Negative Anaerobic BacteriaLittle is known about the effects of stress hormones on the etiologic agents of halitosis. Thus, the aim of this study was to evaluate in vitro the effects of adrenaline (ADR), noradrenaline (NA) and cortisol (CORT) on bacteria that produce volatile sulfur compounds (VSC), the major gases responsible for bad breath. Cultures of Fusobacterium nucleatum (Fn), Porphyromonas endodontalis (Pe), Prevotella intermedia (Pi) and Porphyromonas gingivalis (Pg) were exposed to 50 µM ADR, NA and CORT or equivalent volumes of sterile water as controls for 12 and 24 h. Growth was evaluated based on absorbance at 660 nm. Portable gas chromatography was used to measure VSC concentrations. Kruskal-Wallis and the Dunn post-hoc test were used to compare the groups. For Fn, ADR, NA and CORT significantly reduced bacterial growth after 12 h and 24 h (p < 0.05). All the substances tested increased hydrogen sulfide (H2S) production (p < 0.05). For Pe, all the substances tested reduced bacterial development after 24 h (p < 0.05), and NA significantly increased the H2S concentration after 12 h (p < 0.05). In the Pg and Pi cultures, no effects on bacterial growth were observed (p > 0.05). In the Pi cultures, ADR, NA and CORT increased H2S (p < 0.05). Catecholamines and cortisol can interfere with growth and H2S production of sub-gingival species in vitro. This process appears to be complex and supports the association between stress and the production of VSC.Sociedade Brasileira de Pesquisa Odontológica - SBPqO2014-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242014000100228Brazilian Oral Research v.28 n.1 2014reponame:Brazilian Oral Researchinstname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO)instacron:SBPQO10.1590/1807-3107BOR-2014.vol28.0008info:eu-repo/semantics/openAccessCalil,Caroline MoriniOliveira,Gisele MattosCogo,KarinaPereira,Antonio CarlosMarcondes,Fernanda KleinGroppo,Francisco Carloseng2018-09-17T00:00:00Zoai:scielo:S1806-83242014000100228Revistahttps://www.scielo.br/j/bor/https://old.scielo.br/oai/scielo-oai.phppob@edu.usp.br||bor@sbpqo.org.br1807-31071806-8324opendoar:2018-09-17T00:00Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO)false |
dc.title.none.fl_str_mv |
Effects of stress hormones on the production of volatile sulfur compounds by periodontopathogenic bacteria |
title |
Effects of stress hormones on the production of volatile sulfur compounds by periodontopathogenic bacteria |
spellingShingle |
Effects of stress hormones on the production of volatile sulfur compounds by periodontopathogenic bacteria Calil,Caroline Morini Halitosis Catecholamines Hydrocortisone Gram-Negative Anaerobic Bacteria |
title_short |
Effects of stress hormones on the production of volatile sulfur compounds by periodontopathogenic bacteria |
title_full |
Effects of stress hormones on the production of volatile sulfur compounds by periodontopathogenic bacteria |
title_fullStr |
Effects of stress hormones on the production of volatile sulfur compounds by periodontopathogenic bacteria |
title_full_unstemmed |
Effects of stress hormones on the production of volatile sulfur compounds by periodontopathogenic bacteria |
title_sort |
Effects of stress hormones on the production of volatile sulfur compounds by periodontopathogenic bacteria |
author |
Calil,Caroline Morini |
author_facet |
Calil,Caroline Morini Oliveira,Gisele Mattos Cogo,Karina Pereira,Antonio Carlos Marcondes,Fernanda Klein Groppo,Francisco Carlos |
author_role |
author |
author2 |
Oliveira,Gisele Mattos Cogo,Karina Pereira,Antonio Carlos Marcondes,Fernanda Klein Groppo,Francisco Carlos |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Calil,Caroline Morini Oliveira,Gisele Mattos Cogo,Karina Pereira,Antonio Carlos Marcondes,Fernanda Klein Groppo,Francisco Carlos |
dc.subject.por.fl_str_mv |
Halitosis Catecholamines Hydrocortisone Gram-Negative Anaerobic Bacteria |
topic |
Halitosis Catecholamines Hydrocortisone Gram-Negative Anaerobic Bacteria |
description |
Little is known about the effects of stress hormones on the etiologic agents of halitosis. Thus, the aim of this study was to evaluate in vitro the effects of adrenaline (ADR), noradrenaline (NA) and cortisol (CORT) on bacteria that produce volatile sulfur compounds (VSC), the major gases responsible for bad breath. Cultures of Fusobacterium nucleatum (Fn), Porphyromonas endodontalis (Pe), Prevotella intermedia (Pi) and Porphyromonas gingivalis (Pg) were exposed to 50 µM ADR, NA and CORT or equivalent volumes of sterile water as controls for 12 and 24 h. Growth was evaluated based on absorbance at 660 nm. Portable gas chromatography was used to measure VSC concentrations. Kruskal-Wallis and the Dunn post-hoc test were used to compare the groups. For Fn, ADR, NA and CORT significantly reduced bacterial growth after 12 h and 24 h (p < 0.05). All the substances tested increased hydrogen sulfide (H2S) production (p < 0.05). For Pe, all the substances tested reduced bacterial development after 24 h (p < 0.05), and NA significantly increased the H2S concentration after 12 h (p < 0.05). In the Pg and Pi cultures, no effects on bacterial growth were observed (p > 0.05). In the Pi cultures, ADR, NA and CORT increased H2S (p < 0.05). Catecholamines and cortisol can interfere with growth and H2S production of sub-gingival species in vitro. This process appears to be complex and supports the association between stress and the production of VSC. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242014000100228 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242014000100228 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1807-3107BOR-2014.vol28.0008 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Pesquisa Odontológica - SBPqO |
publisher.none.fl_str_mv |
Sociedade Brasileira de Pesquisa Odontológica - SBPqO |
dc.source.none.fl_str_mv |
Brazilian Oral Research v.28 n.1 2014 reponame:Brazilian Oral Research instname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO) instacron:SBPQO |
instname_str |
Sociedade Brasileira de Pesquisa Odontológica (SBPqO) |
instacron_str |
SBPQO |
institution |
SBPQO |
reponame_str |
Brazilian Oral Research |
collection |
Brazilian Oral Research |
repository.name.fl_str_mv |
Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO) |
repository.mail.fl_str_mv |
pob@edu.usp.br||bor@sbpqo.org.br |
_version_ |
1750318323791298560 |