PAR-2 expression in the gingival crevicular fluid reflects chronic periodontitis severity
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Oral Research |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242017000100214 |
Resumo: | Abstract Recent studies investigating protease-activated receptor type 2 (PAR-2) suggest an association between the receptor and periodontal inflammation. It is known that gingipain, a bacterial protease secreted by the important periodontopathogen Porphyromonas gingivalis can activate PAR-2. Previous studies by our group found that PAR-2 is overexpressed in the gingival crevicular fluid (GCF) of patients with moderate chronic periodontitis (MP). The present study aimed at evaluating whether PAR-2 expression is associated with chronic periodontitis severity. GCF samples and clinical parameters, including plaque and bleeding on probing indices, probing pocket depth and clinical attachment level, were collected from the control group (n = 19) at baseline, and from MP patients (n = 19) and severe chronic periodontitis (SP) (n = 19) patients before and 6 weeks after periodontal non-surgical treatment. PAR-2 and gingipain messenger RNA (mRNA) in the GCF of 4 periodontal sites per patient were evaluated by Reverse Transcription Polymerase Chain Reaction (RT-qPCR). PAR-2 and gingipain expressions were greater in periodontitis patients than in control group patients. In addition, the SP group presented increased PAR-2 and gingipain mRNA levels, compared with the MP group. Furthermore, periodontal treatment significantly reduced (p <0.05) PAR-2 expression in patients with periodontitis. In conclusion, PAR-2 is associated with chronic periodontitis severity and with gingipain levels in the periodontal pocket, thus suggesting that PAR-2 expression in the GCF reflects the severity of destruction during periodontal infection. |
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PAR-2 expression in the gingival crevicular fluid reflects chronic periodontitis severityChronic PeriodontitisReceptor PAR-2Porphyromonas gingivalisArgingipain, Porphyromonas GingivalisAbstract Recent studies investigating protease-activated receptor type 2 (PAR-2) suggest an association between the receptor and periodontal inflammation. It is known that gingipain, a bacterial protease secreted by the important periodontopathogen Porphyromonas gingivalis can activate PAR-2. Previous studies by our group found that PAR-2 is overexpressed in the gingival crevicular fluid (GCF) of patients with moderate chronic periodontitis (MP). The present study aimed at evaluating whether PAR-2 expression is associated with chronic periodontitis severity. GCF samples and clinical parameters, including plaque and bleeding on probing indices, probing pocket depth and clinical attachment level, were collected from the control group (n = 19) at baseline, and from MP patients (n = 19) and severe chronic periodontitis (SP) (n = 19) patients before and 6 weeks after periodontal non-surgical treatment. PAR-2 and gingipain messenger RNA (mRNA) in the GCF of 4 periodontal sites per patient were evaluated by Reverse Transcription Polymerase Chain Reaction (RT-qPCR). PAR-2 and gingipain expressions were greater in periodontitis patients than in control group patients. In addition, the SP group presented increased PAR-2 and gingipain mRNA levels, compared with the MP group. Furthermore, periodontal treatment significantly reduced (p <0.05) PAR-2 expression in patients with periodontitis. In conclusion, PAR-2 is associated with chronic periodontitis severity and with gingipain levels in the periodontal pocket, thus suggesting that PAR-2 expression in the GCF reflects the severity of destruction during periodontal infection.Sociedade Brasileira de Pesquisa Odontológica - SBPqO2017-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242017000100214Brazilian Oral Research v.31 2017reponame:Brazilian Oral Researchinstname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO)instacron:SBPQO10.1590/1807-3107bor-2017.vol31.0016info:eu-repo/semantics/openAccessFUKUSHIMA,HenriqueALVES,Vanessa Tubero EuzebioCARVALHO,Verônica Franco deAMBRÓSIO,Lucas Macedo BatitucciEICHLER,Rosangela Aparecida dos SantosCARVALHO,Maria Helena Catelli deSARAIVA,LucianaHOLZHAUSEN,Marinellaeng2017-01-23T00:00:00Zoai:scielo:S1806-83242017000100214Revistahttps://www.scielo.br/j/bor/https://old.scielo.br/oai/scielo-oai.phppob@edu.usp.br||bor@sbpqo.org.br1807-31071806-8324opendoar:2017-01-23T00:00Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO)false |
dc.title.none.fl_str_mv |
PAR-2 expression in the gingival crevicular fluid reflects chronic periodontitis severity |
title |
PAR-2 expression in the gingival crevicular fluid reflects chronic periodontitis severity |
spellingShingle |
PAR-2 expression in the gingival crevicular fluid reflects chronic periodontitis severity FUKUSHIMA,Henrique Chronic Periodontitis Receptor PAR-2 Porphyromonas gingivalis Argingipain, Porphyromonas Gingivalis |
title_short |
PAR-2 expression in the gingival crevicular fluid reflects chronic periodontitis severity |
title_full |
PAR-2 expression in the gingival crevicular fluid reflects chronic periodontitis severity |
title_fullStr |
PAR-2 expression in the gingival crevicular fluid reflects chronic periodontitis severity |
title_full_unstemmed |
PAR-2 expression in the gingival crevicular fluid reflects chronic periodontitis severity |
title_sort |
PAR-2 expression in the gingival crevicular fluid reflects chronic periodontitis severity |
author |
FUKUSHIMA,Henrique |
author_facet |
FUKUSHIMA,Henrique ALVES,Vanessa Tubero Euzebio CARVALHO,Verônica Franco de AMBRÓSIO,Lucas Macedo Batitucci EICHLER,Rosangela Aparecida dos Santos CARVALHO,Maria Helena Catelli de SARAIVA,Luciana HOLZHAUSEN,Marinella |
author_role |
author |
author2 |
ALVES,Vanessa Tubero Euzebio CARVALHO,Verônica Franco de AMBRÓSIO,Lucas Macedo Batitucci EICHLER,Rosangela Aparecida dos Santos CARVALHO,Maria Helena Catelli de SARAIVA,Luciana HOLZHAUSEN,Marinella |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
FUKUSHIMA,Henrique ALVES,Vanessa Tubero Euzebio CARVALHO,Verônica Franco de AMBRÓSIO,Lucas Macedo Batitucci EICHLER,Rosangela Aparecida dos Santos CARVALHO,Maria Helena Catelli de SARAIVA,Luciana HOLZHAUSEN,Marinella |
dc.subject.por.fl_str_mv |
Chronic Periodontitis Receptor PAR-2 Porphyromonas gingivalis Argingipain, Porphyromonas Gingivalis |
topic |
Chronic Periodontitis Receptor PAR-2 Porphyromonas gingivalis Argingipain, Porphyromonas Gingivalis |
description |
Abstract Recent studies investigating protease-activated receptor type 2 (PAR-2) suggest an association between the receptor and periodontal inflammation. It is known that gingipain, a bacterial protease secreted by the important periodontopathogen Porphyromonas gingivalis can activate PAR-2. Previous studies by our group found that PAR-2 is overexpressed in the gingival crevicular fluid (GCF) of patients with moderate chronic periodontitis (MP). The present study aimed at evaluating whether PAR-2 expression is associated with chronic periodontitis severity. GCF samples and clinical parameters, including plaque and bleeding on probing indices, probing pocket depth and clinical attachment level, were collected from the control group (n = 19) at baseline, and from MP patients (n = 19) and severe chronic periodontitis (SP) (n = 19) patients before and 6 weeks after periodontal non-surgical treatment. PAR-2 and gingipain messenger RNA (mRNA) in the GCF of 4 periodontal sites per patient were evaluated by Reverse Transcription Polymerase Chain Reaction (RT-qPCR). PAR-2 and gingipain expressions were greater in periodontitis patients than in control group patients. In addition, the SP group presented increased PAR-2 and gingipain mRNA levels, compared with the MP group. Furthermore, periodontal treatment significantly reduced (p <0.05) PAR-2 expression in patients with periodontitis. In conclusion, PAR-2 is associated with chronic periodontitis severity and with gingipain levels in the periodontal pocket, thus suggesting that PAR-2 expression in the GCF reflects the severity of destruction during periodontal infection. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242017000100214 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242017000100214 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1807-3107bor-2017.vol31.0016 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Pesquisa Odontológica - SBPqO |
publisher.none.fl_str_mv |
Sociedade Brasileira de Pesquisa Odontológica - SBPqO |
dc.source.none.fl_str_mv |
Brazilian Oral Research v.31 2017 reponame:Brazilian Oral Research instname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO) instacron:SBPQO |
instname_str |
Sociedade Brasileira de Pesquisa Odontológica (SBPqO) |
instacron_str |
SBPQO |
institution |
SBPQO |
reponame_str |
Brazilian Oral Research |
collection |
Brazilian Oral Research |
repository.name.fl_str_mv |
Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO) |
repository.mail.fl_str_mv |
pob@edu.usp.br||bor@sbpqo.org.br |
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1750318325233090560 |