Cytokines and chemokines associated with Treg/Th17 response in chronic inflammatory periapical disease
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Oral Research |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242019000100274 |
Resumo: | Abstract Cytokines and chemokines have a fundamental role in the maintenance of inflammation and bone response, which culminate in the development of chronic periapical lesions. Regulatory (Treg) and Th17 cytokines play a key role in regulating the immune response involved in this process. The aim of this study was to investigate the role of Treg and Th17 cells in chronic inflammatory periapical disease, by comparing the expression of the immunoregulatory mediators TGF-β, IL-10, CCL4, and the proinflammatory IL-17 and CCL20 in the periapical tissue of teeth with pulp necrosis, with and without associated chronic lesions. Eighty-six periapical tissue samples were obtained from human teeth. The samples were divided into three groups: pulp necrosis with a periapical lesion (n=26); pulp necrosis without a periapical lesion (n=30), and control (n=30). All samples were submitted to histopathological analysis and cytokine and chemokine measurement through ELISA. Statistical analyses were done with Kruskal-Wallis and Mann-Whitney tests and Spearman correlation. The group with pulp necrosis and a periapical lesion showed a higher expression of CCL4 and TGF-β in comparison with pulp necrosis without a lesion. CCL20 was higher in the group with a periapical lesion when compared to the control. In all groups there was a weak positive correlation between IL-17/CCL20, IL-10/CCL4, and IL-17/TGF-β. Both types of cytokines, pro-inflammatory and immunoregulatory, occur simultaneously in periapical tissue. However, a rise in immunosuppressive cytokines and chemokines (CCL4 and TGF-β) in periapical lesions suggests a role of these cytokines in stable periapical disease. |
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Cytokines and chemokines associated with Treg/Th17 response in chronic inflammatory periapical diseaseCytokinesEndodonticsPeriapical DiseasesT-lymphocytes, RegulatoryTh17 CellsAbstract Cytokines and chemokines have a fundamental role in the maintenance of inflammation and bone response, which culminate in the development of chronic periapical lesions. Regulatory (Treg) and Th17 cytokines play a key role in regulating the immune response involved in this process. The aim of this study was to investigate the role of Treg and Th17 cells in chronic inflammatory periapical disease, by comparing the expression of the immunoregulatory mediators TGF-β, IL-10, CCL4, and the proinflammatory IL-17 and CCL20 in the periapical tissue of teeth with pulp necrosis, with and without associated chronic lesions. Eighty-six periapical tissue samples were obtained from human teeth. The samples were divided into three groups: pulp necrosis with a periapical lesion (n=26); pulp necrosis without a periapical lesion (n=30), and control (n=30). All samples were submitted to histopathological analysis and cytokine and chemokine measurement through ELISA. Statistical analyses were done with Kruskal-Wallis and Mann-Whitney tests and Spearman correlation. The group with pulp necrosis and a periapical lesion showed a higher expression of CCL4 and TGF-β in comparison with pulp necrosis without a lesion. CCL20 was higher in the group with a periapical lesion when compared to the control. In all groups there was a weak positive correlation between IL-17/CCL20, IL-10/CCL4, and IL-17/TGF-β. Both types of cytokines, pro-inflammatory and immunoregulatory, occur simultaneously in periapical tissue. However, a rise in immunosuppressive cytokines and chemokines (CCL4 and TGF-β) in periapical lesions suggests a role of these cytokines in stable periapical disease.Sociedade Brasileira de Pesquisa Odontológica - SBPqO2019-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242019000100274Brazilian Oral Research v.33 2019reponame:Brazilian Oral Researchinstname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO)instacron:SBPQO10.1590/1807-3107bor-2019.vol33.0093info:eu-repo/semantics/openAccessTOLEDO,André Oliveira Naufel deCOUTO,Aline Maria doMADEIRA,Mila Fernandes MoreiraCALDEIRA,Patrícia CarlosQUEIROZ-JUNIOR,Celso MartinsAGUIAR,Maria Cássia Ferreira deeng2019-10-24T00:00:00Zoai:scielo:S1806-83242019000100274Revistahttps://www.scielo.br/j/bor/https://old.scielo.br/oai/scielo-oai.phppob@edu.usp.br||bor@sbpqo.org.br1807-31071806-8324opendoar:2019-10-24T00:00Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO)false |
dc.title.none.fl_str_mv |
Cytokines and chemokines associated with Treg/Th17 response in chronic inflammatory periapical disease |
title |
Cytokines and chemokines associated with Treg/Th17 response in chronic inflammatory periapical disease |
spellingShingle |
Cytokines and chemokines associated with Treg/Th17 response in chronic inflammatory periapical disease TOLEDO,André Oliveira Naufel de Cytokines Endodontics Periapical Diseases T-lymphocytes, Regulatory Th17 Cells |
title_short |
Cytokines and chemokines associated with Treg/Th17 response in chronic inflammatory periapical disease |
title_full |
Cytokines and chemokines associated with Treg/Th17 response in chronic inflammatory periapical disease |
title_fullStr |
Cytokines and chemokines associated with Treg/Th17 response in chronic inflammatory periapical disease |
title_full_unstemmed |
Cytokines and chemokines associated with Treg/Th17 response in chronic inflammatory periapical disease |
title_sort |
Cytokines and chemokines associated with Treg/Th17 response in chronic inflammatory periapical disease |
author |
TOLEDO,André Oliveira Naufel de |
author_facet |
TOLEDO,André Oliveira Naufel de COUTO,Aline Maria do MADEIRA,Mila Fernandes Moreira CALDEIRA,Patrícia Carlos QUEIROZ-JUNIOR,Celso Martins AGUIAR,Maria Cássia Ferreira de |
author_role |
author |
author2 |
COUTO,Aline Maria do MADEIRA,Mila Fernandes Moreira CALDEIRA,Patrícia Carlos QUEIROZ-JUNIOR,Celso Martins AGUIAR,Maria Cássia Ferreira de |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
TOLEDO,André Oliveira Naufel de COUTO,Aline Maria do MADEIRA,Mila Fernandes Moreira CALDEIRA,Patrícia Carlos QUEIROZ-JUNIOR,Celso Martins AGUIAR,Maria Cássia Ferreira de |
dc.subject.por.fl_str_mv |
Cytokines Endodontics Periapical Diseases T-lymphocytes, Regulatory Th17 Cells |
topic |
Cytokines Endodontics Periapical Diseases T-lymphocytes, Regulatory Th17 Cells |
description |
Abstract Cytokines and chemokines have a fundamental role in the maintenance of inflammation and bone response, which culminate in the development of chronic periapical lesions. Regulatory (Treg) and Th17 cytokines play a key role in regulating the immune response involved in this process. The aim of this study was to investigate the role of Treg and Th17 cells in chronic inflammatory periapical disease, by comparing the expression of the immunoregulatory mediators TGF-β, IL-10, CCL4, and the proinflammatory IL-17 and CCL20 in the periapical tissue of teeth with pulp necrosis, with and without associated chronic lesions. Eighty-six periapical tissue samples were obtained from human teeth. The samples were divided into three groups: pulp necrosis with a periapical lesion (n=26); pulp necrosis without a periapical lesion (n=30), and control (n=30). All samples were submitted to histopathological analysis and cytokine and chemokine measurement through ELISA. Statistical analyses were done with Kruskal-Wallis and Mann-Whitney tests and Spearman correlation. The group with pulp necrosis and a periapical lesion showed a higher expression of CCL4 and TGF-β in comparison with pulp necrosis without a lesion. CCL20 was higher in the group with a periapical lesion when compared to the control. In all groups there was a weak positive correlation between IL-17/CCL20, IL-10/CCL4, and IL-17/TGF-β. Both types of cytokines, pro-inflammatory and immunoregulatory, occur simultaneously in periapical tissue. However, a rise in immunosuppressive cytokines and chemokines (CCL4 and TGF-β) in periapical lesions suggests a role of these cytokines in stable periapical disease. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242019000100274 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242019000100274 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1807-3107bor-2019.vol33.0093 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Pesquisa Odontológica - SBPqO |
publisher.none.fl_str_mv |
Sociedade Brasileira de Pesquisa Odontológica - SBPqO |
dc.source.none.fl_str_mv |
Brazilian Oral Research v.33 2019 reponame:Brazilian Oral Research instname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO) instacron:SBPQO |
instname_str |
Sociedade Brasileira de Pesquisa Odontológica (SBPqO) |
instacron_str |
SBPQO |
institution |
SBPQO |
reponame_str |
Brazilian Oral Research |
collection |
Brazilian Oral Research |
repository.name.fl_str_mv |
Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO) |
repository.mail.fl_str_mv |
pob@edu.usp.br||bor@sbpqo.org.br |
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1750318326632939520 |