Lack of association between viral load and severity of acute bronchiolitis in infants

Detalhes bibliográficos
Autor(a) principal: Souza,Ana Paula Duarte de
Data de Publicação: 2016
Outros Autores: Leitão,Lidiane Alves de Azeredo, Luisi,Fernanda, Souza,Rodrigo Godinho, Coutinho,Sandra Eugênia, Silva,Jaqueline Ramos da, Mattiello,Rita, Pitrez,Paulo Márcio Condessa, Stein,Renato Tetelbom, Pinto,Leonardo Araújo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Jornal Brasileiro de Pneumologia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-37132016000400261
Resumo: ABSTRACT Objective: To investigate the correlation between respiratory syncytial viral load and length of hospitalization in infants with acute wheezing episodes. Methods: This was a two-year, cross-sectional study of infants ≤ 12 months of age with bronchiolitis at the time of admission to a tertiary hospital. For the identification of respiratory viruses, nasopharyngeal secretions were collected. Samples were analyzed (throughout the study period) by direct immunofluorescence and (in the second year of the study) by quantitative real-time PCR. We screened for three human viruses: rhinovirus, respiratory syncytial virus, and metapneumovirus. Results: Of 110 samples evaluated by direct immunofluorescence, 56 (50.9%) were positive for a single virus, and 16 (14.5%) were positive for two or more viruses. Among those 72 samples, the most prevalent virus was respiratory syncytial virus, followed by influenza. Of 56 samples evaluated by quantitative real-time PCR, 24 (42.8%) were positive for a single virus, and 1 (1.7%) was positive for two viruses. Among those 25 samples, the most prevalent virus was again respiratory syncytial virus, followed by human rhinovirus. Coinfection did not influence the length of the hospital stay or other outcome s. In addition, there was no association between respiratory syncytial virus load and the length of hospitalization. Conclusions: Neither coinfection nor respiratory syncytial viral load appears to influence the outcomes of acute bronchiolitis in infants.
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spelling Lack of association between viral load and severity of acute bronchiolitis in infantsBronchiolitisCoinfectionViral loadHospitalizationRespiratory syncytial virus, humanABSTRACT Objective: To investigate the correlation between respiratory syncytial viral load and length of hospitalization in infants with acute wheezing episodes. Methods: This was a two-year, cross-sectional study of infants ≤ 12 months of age with bronchiolitis at the time of admission to a tertiary hospital. For the identification of respiratory viruses, nasopharyngeal secretions were collected. Samples were analyzed (throughout the study period) by direct immunofluorescence and (in the second year of the study) by quantitative real-time PCR. We screened for three human viruses: rhinovirus, respiratory syncytial virus, and metapneumovirus. Results: Of 110 samples evaluated by direct immunofluorescence, 56 (50.9%) were positive for a single virus, and 16 (14.5%) were positive for two or more viruses. Among those 72 samples, the most prevalent virus was respiratory syncytial virus, followed by influenza. Of 56 samples evaluated by quantitative real-time PCR, 24 (42.8%) were positive for a single virus, and 1 (1.7%) was positive for two viruses. Among those 25 samples, the most prevalent virus was again respiratory syncytial virus, followed by human rhinovirus. Coinfection did not influence the length of the hospital stay or other outcome s. In addition, there was no association between respiratory syncytial virus load and the length of hospitalization. Conclusions: Neither coinfection nor respiratory syncytial viral load appears to influence the outcomes of acute bronchiolitis in infants.Sociedade Brasileira de Pneumologia e Tisiologia2016-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-37132016000400261Jornal Brasileiro de Pneumologia v.42 n.4 2016reponame:Jornal Brasileiro de Pneumologia (Online)instname:Sociedade Brasileira de Pneumologia e Tisiologia (SBPT)instacron:SBPT10.1590/s1806-37562015000000241info:eu-repo/semantics/openAccessSouza,Ana Paula Duarte deLeitão,Lidiane Alves de AzeredoLuisi,FernandaSouza,Rodrigo GodinhoCoutinho,Sandra EugêniaSilva,Jaqueline Ramos daMattiello,RitaPitrez,Paulo Márcio CondessaStein,Renato TetelbomPinto,Leonardo Araújoeng2017-03-10T00:00:00Zoai:scielo:S1806-37132016000400261Revistahttp://www.jornaldepneumologia.com.br/default.aspONGhttps://old.scielo.br/oai/scielo-oai.php||jbp@jbp.org.br|| jpneumo@jornaldepneumologia.com.br1806-37561806-3713opendoar:2017-03-10T00:00Jornal Brasileiro de Pneumologia (Online) - Sociedade Brasileira de Pneumologia e Tisiologia (SBPT)false
dc.title.none.fl_str_mv Lack of association between viral load and severity of acute bronchiolitis in infants
title Lack of association between viral load and severity of acute bronchiolitis in infants
spellingShingle Lack of association between viral load and severity of acute bronchiolitis in infants
Souza,Ana Paula Duarte de
Bronchiolitis
Coinfection
Viral load
Hospitalization
Respiratory syncytial virus, human
title_short Lack of association between viral load and severity of acute bronchiolitis in infants
title_full Lack of association between viral load and severity of acute bronchiolitis in infants
title_fullStr Lack of association between viral load and severity of acute bronchiolitis in infants
title_full_unstemmed Lack of association between viral load and severity of acute bronchiolitis in infants
title_sort Lack of association between viral load and severity of acute bronchiolitis in infants
author Souza,Ana Paula Duarte de
author_facet Souza,Ana Paula Duarte de
Leitão,Lidiane Alves de Azeredo
Luisi,Fernanda
Souza,Rodrigo Godinho
Coutinho,Sandra Eugênia
Silva,Jaqueline Ramos da
Mattiello,Rita
Pitrez,Paulo Márcio Condessa
Stein,Renato Tetelbom
Pinto,Leonardo Araújo
author_role author
author2 Leitão,Lidiane Alves de Azeredo
Luisi,Fernanda
Souza,Rodrigo Godinho
Coutinho,Sandra Eugênia
Silva,Jaqueline Ramos da
Mattiello,Rita
Pitrez,Paulo Márcio Condessa
Stein,Renato Tetelbom
Pinto,Leonardo Araújo
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Souza,Ana Paula Duarte de
Leitão,Lidiane Alves de Azeredo
Luisi,Fernanda
Souza,Rodrigo Godinho
Coutinho,Sandra Eugênia
Silva,Jaqueline Ramos da
Mattiello,Rita
Pitrez,Paulo Márcio Condessa
Stein,Renato Tetelbom
Pinto,Leonardo Araújo
dc.subject.por.fl_str_mv Bronchiolitis
Coinfection
Viral load
Hospitalization
Respiratory syncytial virus, human
topic Bronchiolitis
Coinfection
Viral load
Hospitalization
Respiratory syncytial virus, human
description ABSTRACT Objective: To investigate the correlation between respiratory syncytial viral load and length of hospitalization in infants with acute wheezing episodes. Methods: This was a two-year, cross-sectional study of infants ≤ 12 months of age with bronchiolitis at the time of admission to a tertiary hospital. For the identification of respiratory viruses, nasopharyngeal secretions were collected. Samples were analyzed (throughout the study period) by direct immunofluorescence and (in the second year of the study) by quantitative real-time PCR. We screened for three human viruses: rhinovirus, respiratory syncytial virus, and metapneumovirus. Results: Of 110 samples evaluated by direct immunofluorescence, 56 (50.9%) were positive for a single virus, and 16 (14.5%) were positive for two or more viruses. Among those 72 samples, the most prevalent virus was respiratory syncytial virus, followed by influenza. Of 56 samples evaluated by quantitative real-time PCR, 24 (42.8%) were positive for a single virus, and 1 (1.7%) was positive for two viruses. Among those 25 samples, the most prevalent virus was again respiratory syncytial virus, followed by human rhinovirus. Coinfection did not influence the length of the hospital stay or other outcome s. In addition, there was no association between respiratory syncytial virus load and the length of hospitalization. Conclusions: Neither coinfection nor respiratory syncytial viral load appears to influence the outcomes of acute bronchiolitis in infants.
publishDate 2016
dc.date.none.fl_str_mv 2016-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-37132016000400261
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dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/s1806-37562015000000241
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Pneumologia e Tisiologia
publisher.none.fl_str_mv Sociedade Brasileira de Pneumologia e Tisiologia
dc.source.none.fl_str_mv Jornal Brasileiro de Pneumologia v.42 n.4 2016
reponame:Jornal Brasileiro de Pneumologia (Online)
instname:Sociedade Brasileira de Pneumologia e Tisiologia (SBPT)
instacron:SBPT
instname_str Sociedade Brasileira de Pneumologia e Tisiologia (SBPT)
instacron_str SBPT
institution SBPT
reponame_str Jornal Brasileiro de Pneumologia (Online)
collection Jornal Brasileiro de Pneumologia (Online)
repository.name.fl_str_mv Jornal Brasileiro de Pneumologia (Online) - Sociedade Brasileira de Pneumologia e Tisiologia (SBPT)
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