Ultrasound-Assisted Saponification Coupled with Gas Chromatography-Flame Ionization Detection for the Determination of Phytosterols from Passion Fruit Seed Oil

• Autor(a) principal: Rotta,Eliza M.
• Data de Publicação: 2018
• Outros Autores: Silva,Michele C. da, Maldaner,Liane, Visentainer,Jesuí V.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Journal of the Brazilian Chemical Society (Online)
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532018000300579
• topic: Passiflora species; campesterol; β-sitosterol; stigmasterol

An ultrasound-assisted saponification (UAS) method for the determination of phytosterols (campesterol, β-sitosterol and stigmasterol) in passion fruit seed oil, followed by gas chromatography with flame ionization detection, was developed and validated. Under the optimum conditions, good linearity with correlation coefficients higher than 0.99 was obtained for all analytes. The recoveries for the proposed approach were between 103 and 107%, the limits of quantification were between 70 and 180 μg kg-1 and the precision values expressed in terms of relative standard deviations (RSD) were lower than 19%. The developed method was successfully applied to the analysis of passion fruit seed oil samples from different species, including yellow passion fruit, sweet granadilla and sweet passion fruit. In all species of analyzed passion fruit seed oils, β-sitosterol was found with the highest abundance, ranging from 0.90 ± 0.02 to 1.60 ± 0.01 mg g-1 of oil, followed by stigmasterol (0.70 ± 0.01 to 1.40 ± 0.12 mg g-1 of oil) and campesterol (0.10 ± 0.01 to 0.30 ± 0.06 mg g-1 of oil). Furthermore, the developed method proved to be a time-saving and efficient analytical procedure for the determination of phytosterols in oil samples.