Esterase screening using whole cells of Brazilian soil microorganisms

Detalhes bibliográficos
Autor(a) principal: Mantovani,Simone M.
Data de Publicação: 2010
Outros Autores: Oliveira,Luciana G. de, Marsaioli,Anita J.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of the Brazilian Chemical Society (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532010000800011
Resumo: A miniaturized enzymatic assay using fluorescent probes to reveal esterase producing microorganisms was optimized and applied to screen 64 soil bacterial strains. The best results were validated using traditional non-fluorogenic assays with acetyl and propanoyl phenylethanol to confirm the miniaturized results. The most active microorganisms belong to the genus Bacillus showing esterase activity and good enantiomeric ratios for the resolution of phenylethanol derivatives (E > 30). Part of the microorganisms are kept in our laboratory in glycerol or freeze-dried and the best microorganisms will be deposited in the CBMAI/CPQBA/Unicamp culture collection.
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spelling Esterase screening using whole cells of Brazilian soil microorganismssoil bacteriaminiaturized screening with fluorescent probesesterasesA miniaturized enzymatic assay using fluorescent probes to reveal esterase producing microorganisms was optimized and applied to screen 64 soil bacterial strains. The best results were validated using traditional non-fluorogenic assays with acetyl and propanoyl phenylethanol to confirm the miniaturized results. The most active microorganisms belong to the genus Bacillus showing esterase activity and good enantiomeric ratios for the resolution of phenylethanol derivatives (E > 30). Part of the microorganisms are kept in our laboratory in glycerol or freeze-dried and the best microorganisms will be deposited in the CBMAI/CPQBA/Unicamp culture collection.Sociedade Brasileira de Química2010-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532010000800011Journal of the Brazilian Chemical Society v.21 n.8 2010reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.1590/S0103-50532010000800011info:eu-repo/semantics/openAccessMantovani,Simone M.Oliveira,Luciana G. deMarsaioli,Anita J.eng2011-10-14T00:00:00Zoai:scielo:S0103-50532010000800011Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2011-10-14T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv Esterase screening using whole cells of Brazilian soil microorganisms
title Esterase screening using whole cells of Brazilian soil microorganisms
spellingShingle Esterase screening using whole cells of Brazilian soil microorganisms
Mantovani,Simone M.
soil bacteria
miniaturized screening with fluorescent probes
esterases
title_short Esterase screening using whole cells of Brazilian soil microorganisms
title_full Esterase screening using whole cells of Brazilian soil microorganisms
title_fullStr Esterase screening using whole cells of Brazilian soil microorganisms
title_full_unstemmed Esterase screening using whole cells of Brazilian soil microorganisms
title_sort Esterase screening using whole cells of Brazilian soil microorganisms
author Mantovani,Simone M.
author_facet Mantovani,Simone M.
Oliveira,Luciana G. de
Marsaioli,Anita J.
author_role author
author2 Oliveira,Luciana G. de
Marsaioli,Anita J.
author2_role author
author
dc.contributor.author.fl_str_mv Mantovani,Simone M.
Oliveira,Luciana G. de
Marsaioli,Anita J.
dc.subject.por.fl_str_mv soil bacteria
miniaturized screening with fluorescent probes
esterases
topic soil bacteria
miniaturized screening with fluorescent probes
esterases
description A miniaturized enzymatic assay using fluorescent probes to reveal esterase producing microorganisms was optimized and applied to screen 64 soil bacterial strains. The best results were validated using traditional non-fluorogenic assays with acetyl and propanoyl phenylethanol to confirm the miniaturized results. The most active microorganisms belong to the genus Bacillus showing esterase activity and good enantiomeric ratios for the resolution of phenylethanol derivatives (E > 30). Part of the microorganisms are kept in our laboratory in glycerol or freeze-dried and the best microorganisms will be deposited in the CBMAI/CPQBA/Unicamp culture collection.
publishDate 2010
dc.date.none.fl_str_mv 2010-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532010000800011
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532010000800011
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0103-50532010000800011
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Journal of the Brazilian Chemical Society v.21 n.8 2010
reponame:Journal of the Brazilian Chemical Society (Online)
instname:Sociedade Brasileira de Química (SBQ)
instacron:SBQ
instname_str Sociedade Brasileira de Química (SBQ)
instacron_str SBQ
institution SBQ
reponame_str Journal of the Brazilian Chemical Society (Online)
collection Journal of the Brazilian Chemical Society (Online)
repository.name.fl_str_mv Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)
repository.mail.fl_str_mv ||office@jbcs.sbq.org.br
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