A Simple and Fast Method for Magnetic Solid Phase Extraction of Ochratoxin A
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of the Brazilian Chemical Society (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532017000600950 |
Resumo: | We report on the synthesis of dopamine loaded magnetic nanoparticles (MNPs) for new, simple, fast and repeatable extraction of ochratoxin A from different solvents and milk without utilizing immunoaffinity columns and even high-tech devices. To this end, Fe3O4 nanoparticles (NPs) were synthesized using thermal decomposition reaction and dopamine (DPA) was then conjugated with Fe3O4 nanoparticles (NPs) to form Fe3O4-DPA NPs. Dynamic light scattering, field emission scanning electron microscopy and transmission electron microscopy revealed an average size of about 15 nm for Fe3O4-DPA NPs. Moreover, zeta potential measurement and vibrating sample magnetometer confirmed positively charged (16.8 mV) and superparamagnetic behavior of MNPs, which are effective factors for a good adsorbent in the extraction. Various solvents and different effective parameters were measured until acetonitrile:methanol was selected as the best extraction solvent. In addition, based on the pH-partition theory, with changes in pH, we were able to increase and enhance the extraction rate to 94%. Moreover, the ability of Fe3O4-DPA NPs in solid phase extraction of ochratoxin A from spiked milk was evaluated. The recovery rate for extraction of OTA from milk was 68%. |
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A Simple and Fast Method for Magnetic Solid Phase Extraction of Ochratoxin Aochratoxin Amagnetic nanoparticlesHPLCsolid phase extractionfluorescence spectroscopyWe report on the synthesis of dopamine loaded magnetic nanoparticles (MNPs) for new, simple, fast and repeatable extraction of ochratoxin A from different solvents and milk without utilizing immunoaffinity columns and even high-tech devices. To this end, Fe3O4 nanoparticles (NPs) were synthesized using thermal decomposition reaction and dopamine (DPA) was then conjugated with Fe3O4 nanoparticles (NPs) to form Fe3O4-DPA NPs. Dynamic light scattering, field emission scanning electron microscopy and transmission electron microscopy revealed an average size of about 15 nm for Fe3O4-DPA NPs. Moreover, zeta potential measurement and vibrating sample magnetometer confirmed positively charged (16.8 mV) and superparamagnetic behavior of MNPs, which are effective factors for a good adsorbent in the extraction. Various solvents and different effective parameters were measured until acetonitrile:methanol was selected as the best extraction solvent. In addition, based on the pH-partition theory, with changes in pH, we were able to increase and enhance the extraction rate to 94%. Moreover, the ability of Fe3O4-DPA NPs in solid phase extraction of ochratoxin A from spiked milk was evaluated. The recovery rate for extraction of OTA from milk was 68%.Sociedade Brasileira de Química2017-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532017000600950Journal of the Brazilian Chemical Society v.28 n.6 2017reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.21577/0103-5053.20160245info:eu-repo/semantics/openAccessSargazi,AzamAliabadi,AminRahdari,AliAllahdini-Hesaroiyeh,SaminNejati-Yazdinejad,MassoudMajd,Mostafa Heidarieng2017-05-11T00:00:00Zoai:scielo:S0103-50532017000600950Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2017-05-11T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false |
dc.title.none.fl_str_mv |
A Simple and Fast Method for Magnetic Solid Phase Extraction of Ochratoxin A |
title |
A Simple and Fast Method for Magnetic Solid Phase Extraction of Ochratoxin A |
spellingShingle |
A Simple and Fast Method for Magnetic Solid Phase Extraction of Ochratoxin A Sargazi,Azam ochratoxin A magnetic nanoparticles HPLC solid phase extraction fluorescence spectroscopy |
title_short |
A Simple and Fast Method for Magnetic Solid Phase Extraction of Ochratoxin A |
title_full |
A Simple and Fast Method for Magnetic Solid Phase Extraction of Ochratoxin A |
title_fullStr |
A Simple and Fast Method for Magnetic Solid Phase Extraction of Ochratoxin A |
title_full_unstemmed |
A Simple and Fast Method for Magnetic Solid Phase Extraction of Ochratoxin A |
title_sort |
A Simple and Fast Method for Magnetic Solid Phase Extraction of Ochratoxin A |
author |
Sargazi,Azam |
author_facet |
Sargazi,Azam Aliabadi,Amin Rahdari,Ali Allahdini-Hesaroiyeh,Samin Nejati-Yazdinejad,Massoud Majd,Mostafa Heidari |
author_role |
author |
author2 |
Aliabadi,Amin Rahdari,Ali Allahdini-Hesaroiyeh,Samin Nejati-Yazdinejad,Massoud Majd,Mostafa Heidari |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Sargazi,Azam Aliabadi,Amin Rahdari,Ali Allahdini-Hesaroiyeh,Samin Nejati-Yazdinejad,Massoud Majd,Mostafa Heidari |
dc.subject.por.fl_str_mv |
ochratoxin A magnetic nanoparticles HPLC solid phase extraction fluorescence spectroscopy |
topic |
ochratoxin A magnetic nanoparticles HPLC solid phase extraction fluorescence spectroscopy |
description |
We report on the synthesis of dopamine loaded magnetic nanoparticles (MNPs) for new, simple, fast and repeatable extraction of ochratoxin A from different solvents and milk without utilizing immunoaffinity columns and even high-tech devices. To this end, Fe3O4 nanoparticles (NPs) were synthesized using thermal decomposition reaction and dopamine (DPA) was then conjugated with Fe3O4 nanoparticles (NPs) to form Fe3O4-DPA NPs. Dynamic light scattering, field emission scanning electron microscopy and transmission electron microscopy revealed an average size of about 15 nm for Fe3O4-DPA NPs. Moreover, zeta potential measurement and vibrating sample magnetometer confirmed positively charged (16.8 mV) and superparamagnetic behavior of MNPs, which are effective factors for a good adsorbent in the extraction. Various solvents and different effective parameters were measured until acetonitrile:methanol was selected as the best extraction solvent. In addition, based on the pH-partition theory, with changes in pH, we were able to increase and enhance the extraction rate to 94%. Moreover, the ability of Fe3O4-DPA NPs in solid phase extraction of ochratoxin A from spiked milk was evaluated. The recovery rate for extraction of OTA from milk was 68%. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532017000600950 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532017000600950 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.21577/0103-5053.20160245 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
dc.source.none.fl_str_mv |
Journal of the Brazilian Chemical Society v.28 n.6 2017 reponame:Journal of the Brazilian Chemical Society (Online) instname:Sociedade Brasileira de Química (SBQ) instacron:SBQ |
instname_str |
Sociedade Brasileira de Química (SBQ) |
instacron_str |
SBQ |
institution |
SBQ |
reponame_str |
Journal of the Brazilian Chemical Society (Online) |
collection |
Journal of the Brazilian Chemical Society (Online) |
repository.name.fl_str_mv |
Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ) |
repository.mail.fl_str_mv |
||office@jbcs.sbq.org.br |
_version_ |
1750318179553378304 |