Rapid and variable-volume sampling loading in nonaqueous microchip electrophoresis
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of the Brazilian Chemical Society (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532014000400019 |
Resumo: | A rapid and variable-volume sample loading scheme for nonaqueous microchip electrophoresis with laser induced fluorescence detection (NAMCE-LIF) was developed, which included a porous polymer plug, a low-cost microvacuum pump, and a single potential supply. The porous polymer plug was fabricated within the separation channel as a select valve, which prevented the running buffer from buffer waste reservoir (BW) flowing back into the separation channel, but allowed electrophoretic migration along the separation channel. By applying a subatmospheric pressure to the headspace of sample waste reservoir (SW), sample and running buffer were drawn immediately to SW. At the same time, a branch of the sample stream was driven across the porous polymer plug by the electric force and formed a sample zone in the separation channel. The injected sample zone length was in proportion with the injection time. The proposed method was successfully applied to separation of Rhodamine 123 (Rh123) and Rhodamine 6G (Rh6G). The limits of detection for Rh123 and Rh6G based on S/N = 3 were 0.63 and 0.48 nmol L- 1, respectively. The system has been proved to possess an exciting potential for improving throughput, repeatability, sensitivity, separation performance of nonaqueous microchip electrophoresis and a wide range of applications. |
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Rapid and variable-volume sampling loading in nonaqueous microchip electrophoresisvariable-volume sample loadingnonaqueous microchip electrophoresisnegative pressureporous polymer plugelectrokinetic forceA rapid and variable-volume sample loading scheme for nonaqueous microchip electrophoresis with laser induced fluorescence detection (NAMCE-LIF) was developed, which included a porous polymer plug, a low-cost microvacuum pump, and a single potential supply. The porous polymer plug was fabricated within the separation channel as a select valve, which prevented the running buffer from buffer waste reservoir (BW) flowing back into the separation channel, but allowed electrophoretic migration along the separation channel. By applying a subatmospheric pressure to the headspace of sample waste reservoir (SW), sample and running buffer were drawn immediately to SW. At the same time, a branch of the sample stream was driven across the porous polymer plug by the electric force and formed a sample zone in the separation channel. The injected sample zone length was in proportion with the injection time. The proposed method was successfully applied to separation of Rhodamine 123 (Rh123) and Rhodamine 6G (Rh6G). The limits of detection for Rh123 and Rh6G based on S/N = 3 were 0.63 and 0.48 nmol L- 1, respectively. The system has been proved to possess an exciting potential for improving throughput, repeatability, sensitivity, separation performance of nonaqueous microchip electrophoresis and a wide range of applications.Sociedade Brasileira de Química2014-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532014000400019Journal of the Brazilian Chemical Society v.25 n.4 2014reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.5935/0103-5053.20140038info:eu-repo/semantics/openAccessLi,ZhenhuaGuo,YuanmingZhou,YongdongZhang,XiaoningHu,Hongmeieng2014-04-22T00:00:00Zoai:scielo:S0103-50532014000400019Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2014-04-22T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false |
dc.title.none.fl_str_mv |
Rapid and variable-volume sampling loading in nonaqueous microchip electrophoresis |
title |
Rapid and variable-volume sampling loading in nonaqueous microchip electrophoresis |
spellingShingle |
Rapid and variable-volume sampling loading in nonaqueous microchip electrophoresis Li,Zhenhua variable-volume sample loading nonaqueous microchip electrophoresis negative pressure porous polymer plug electrokinetic force |
title_short |
Rapid and variable-volume sampling loading in nonaqueous microchip electrophoresis |
title_full |
Rapid and variable-volume sampling loading in nonaqueous microchip electrophoresis |
title_fullStr |
Rapid and variable-volume sampling loading in nonaqueous microchip electrophoresis |
title_full_unstemmed |
Rapid and variable-volume sampling loading in nonaqueous microchip electrophoresis |
title_sort |
Rapid and variable-volume sampling loading in nonaqueous microchip electrophoresis |
author |
Li,Zhenhua |
author_facet |
Li,Zhenhua Guo,Yuanming Zhou,Yongdong Zhang,Xiaoning Hu,Hongmei |
author_role |
author |
author2 |
Guo,Yuanming Zhou,Yongdong Zhang,Xiaoning Hu,Hongmei |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Li,Zhenhua Guo,Yuanming Zhou,Yongdong Zhang,Xiaoning Hu,Hongmei |
dc.subject.por.fl_str_mv |
variable-volume sample loading nonaqueous microchip electrophoresis negative pressure porous polymer plug electrokinetic force |
topic |
variable-volume sample loading nonaqueous microchip electrophoresis negative pressure porous polymer plug electrokinetic force |
description |
A rapid and variable-volume sample loading scheme for nonaqueous microchip electrophoresis with laser induced fluorescence detection (NAMCE-LIF) was developed, which included a porous polymer plug, a low-cost microvacuum pump, and a single potential supply. The porous polymer plug was fabricated within the separation channel as a select valve, which prevented the running buffer from buffer waste reservoir (BW) flowing back into the separation channel, but allowed electrophoretic migration along the separation channel. By applying a subatmospheric pressure to the headspace of sample waste reservoir (SW), sample and running buffer were drawn immediately to SW. At the same time, a branch of the sample stream was driven across the porous polymer plug by the electric force and formed a sample zone in the separation channel. The injected sample zone length was in proportion with the injection time. The proposed method was successfully applied to separation of Rhodamine 123 (Rh123) and Rhodamine 6G (Rh6G). The limits of detection for Rh123 and Rh6G based on S/N = 3 were 0.63 and 0.48 nmol L- 1, respectively. The system has been proved to possess an exciting potential for improving throughput, repeatability, sensitivity, separation performance of nonaqueous microchip electrophoresis and a wide range of applications. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-04-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532014000400019 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532014000400019 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.5935/0103-5053.20140038 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
dc.source.none.fl_str_mv |
Journal of the Brazilian Chemical Society v.25 n.4 2014 reponame:Journal of the Brazilian Chemical Society (Online) instname:Sociedade Brasileira de Química (SBQ) instacron:SBQ |
instname_str |
Sociedade Brasileira de Química (SBQ) |
instacron_str |
SBQ |
institution |
SBQ |
reponame_str |
Journal of the Brazilian Chemical Society (Online) |
collection |
Journal of the Brazilian Chemical Society (Online) |
repository.name.fl_str_mv |
Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ) |
repository.mail.fl_str_mv |
||office@jbcs.sbq.org.br |
_version_ |
1750318175798427648 |