Extraction and purification of C-phycocyanin from Spirulina platensis in conventional and integrated aqueous two-phase systems
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of the Brazilian Chemical Society (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532010000500022 |
Resumo: | Crude C-phycocyanin extracts from Spirulina platensis, with and without cell debris, were purified in aqueous two-phase systems ( ATPS) varying the molar mass of the PEG, at pH 6, more promising than at pH 7 . For the assays carried out with each molar mass, with distinct percentages of PEG and salt, the concentrations (mg mL-1), purities (OD 620/OD 280) and C-phycocyanin partition coefficients of each system were determined and compared. The process for the primary purification of intracellular C-phycocyanin with cell debris (integrated), composed of 15% of PEG 1500 and 13% of potassium phosphate and 5% of PEG 4000 and 18% of salt, produced C-phycocyanin concentrations of, respectively, 1.60 and 2.67 mg mL-1 in the top phase. The purities were of 0.73 and 0.79 respectively, values higher than those obtained in the systems without cells. |
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Extraction and purification of C-phycocyanin from Spirulina platensis in conventional and integrated aqueous two-phase systemsC-phycocyaninaqueous two-phase systempurityproteinpolyethylene glycolCrude C-phycocyanin extracts from Spirulina platensis, with and without cell debris, were purified in aqueous two-phase systems ( ATPS) varying the molar mass of the PEG, at pH 6, more promising than at pH 7 . For the assays carried out with each molar mass, with distinct percentages of PEG and salt, the concentrations (mg mL-1), purities (OD 620/OD 280) and C-phycocyanin partition coefficients of each system were determined and compared. The process for the primary purification of intracellular C-phycocyanin with cell debris (integrated), composed of 15% of PEG 1500 and 13% of potassium phosphate and 5% of PEG 4000 and 18% of salt, produced C-phycocyanin concentrations of, respectively, 1.60 and 2.67 mg mL-1 in the top phase. The purities were of 0.73 and 0.79 respectively, values higher than those obtained in the systems without cells.Sociedade Brasileira de Química2010-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532010000500022Journal of the Brazilian Chemical Society v.21 n.5 2010reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.1590/S0103-50532010000500022info:eu-repo/semantics/openAccessAntelo,Francine S.Anschau,AndréiaCosta,Jorge A. V.Kalil,Susana J.eng2010-07-13T00:00:00Zoai:scielo:S0103-50532010000500022Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2010-07-13T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false |
dc.title.none.fl_str_mv |
Extraction and purification of C-phycocyanin from Spirulina platensis in conventional and integrated aqueous two-phase systems |
title |
Extraction and purification of C-phycocyanin from Spirulina platensis in conventional and integrated aqueous two-phase systems |
spellingShingle |
Extraction and purification of C-phycocyanin from Spirulina platensis in conventional and integrated aqueous two-phase systems Antelo,Francine S. C-phycocyanin aqueous two-phase system purity protein polyethylene glycol |
title_short |
Extraction and purification of C-phycocyanin from Spirulina platensis in conventional and integrated aqueous two-phase systems |
title_full |
Extraction and purification of C-phycocyanin from Spirulina platensis in conventional and integrated aqueous two-phase systems |
title_fullStr |
Extraction and purification of C-phycocyanin from Spirulina platensis in conventional and integrated aqueous two-phase systems |
title_full_unstemmed |
Extraction and purification of C-phycocyanin from Spirulina platensis in conventional and integrated aqueous two-phase systems |
title_sort |
Extraction and purification of C-phycocyanin from Spirulina platensis in conventional and integrated aqueous two-phase systems |
author |
Antelo,Francine S. |
author_facet |
Antelo,Francine S. Anschau,Andréia Costa,Jorge A. V. Kalil,Susana J. |
author_role |
author |
author2 |
Anschau,Andréia Costa,Jorge A. V. Kalil,Susana J. |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Antelo,Francine S. Anschau,Andréia Costa,Jorge A. V. Kalil,Susana J. |
dc.subject.por.fl_str_mv |
C-phycocyanin aqueous two-phase system purity protein polyethylene glycol |
topic |
C-phycocyanin aqueous two-phase system purity protein polyethylene glycol |
description |
Crude C-phycocyanin extracts from Spirulina platensis, with and without cell debris, were purified in aqueous two-phase systems ( ATPS) varying the molar mass of the PEG, at pH 6, more promising than at pH 7 . For the assays carried out with each molar mass, with distinct percentages of PEG and salt, the concentrations (mg mL-1), purities (OD 620/OD 280) and C-phycocyanin partition coefficients of each system were determined and compared. The process for the primary purification of intracellular C-phycocyanin with cell debris (integrated), composed of 15% of PEG 1500 and 13% of potassium phosphate and 5% of PEG 4000 and 18% of salt, produced C-phycocyanin concentrations of, respectively, 1.60 and 2.67 mg mL-1 in the top phase. The purities were of 0.73 and 0.79 respectively, values higher than those obtained in the systems without cells. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532010000500022 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532010000500022 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0103-50532010000500022 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
dc.source.none.fl_str_mv |
Journal of the Brazilian Chemical Society v.21 n.5 2010 reponame:Journal of the Brazilian Chemical Society (Online) instname:Sociedade Brasileira de Química (SBQ) instacron:SBQ |
instname_str |
Sociedade Brasileira de Química (SBQ) |
instacron_str |
SBQ |
institution |
SBQ |
reponame_str |
Journal of the Brazilian Chemical Society (Online) |
collection |
Journal of the Brazilian Chemical Society (Online) |
repository.name.fl_str_mv |
Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ) |
repository.mail.fl_str_mv |
||office@jbcs.sbq.org.br |
_version_ |
1750318171013775360 |