Development of Surface Plasmon Resonance-Based Immunosensor for Detection of Brucella melitensis

Detalhes bibliográficos
Autor(a) principal: Saberi,Fatemeh
Data de Publicação: 2016
Outros Autores: Kamali,Mehdi, Taheri,Ramezan A., Ramandi,Mahdi F., Bagdeli,Samira, Mirnejad,Reza
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of the Brazilian Chemical Society (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532016001101960
Resumo: A label-free optical immunosensor was developed based on surface plasmon resonance (SPR) method for rapid and selective detection of Brucella melitensis. This immunosensor was constructed by immobilizing capture antibody on 11-mercaptoundecanoic acid modified gold disk. This fabricated immunosensor detected B. melitensis at a concentration range from 103 to 107 cell mL-1 (R2 = 0.998) and a detection limit of 100 cell mL-1. Additionally, the kinetic equilibrium dissociation constant (KD) for assessment of the interaction affinity was calculated as 1.1 × 10-9 mol L-1 that can be considered as high affinity interaction. This SPR immunosensor provided advantages in term of fast response, label free and accurate detection of B. melitensis in analytical systems.
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spelling Development of Surface Plasmon Resonance-Based Immunosensor for Detection of Brucella melitensisBrucellasurface plasmon resonancekineticsbiosensorA label-free optical immunosensor was developed based on surface plasmon resonance (SPR) method for rapid and selective detection of Brucella melitensis. This immunosensor was constructed by immobilizing capture antibody on 11-mercaptoundecanoic acid modified gold disk. This fabricated immunosensor detected B. melitensis at a concentration range from 103 to 107 cell mL-1 (R2 = 0.998) and a detection limit of 100 cell mL-1. Additionally, the kinetic equilibrium dissociation constant (KD) for assessment of the interaction affinity was calculated as 1.1 × 10-9 mol L-1 that can be considered as high affinity interaction. This SPR immunosensor provided advantages in term of fast response, label free and accurate detection of B. melitensis in analytical systems.Sociedade Brasileira de Química2016-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532016001101960Journal of the Brazilian Chemical Society v.27 n.11 2016reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.5935/0103-5053.20160085info:eu-repo/semantics/openAccessSaberi,FatemehKamali,MehdiTaheri,Ramezan A.Ramandi,Mahdi F.Bagdeli,SamiraMirnejad,Rezaeng2016-11-04T00:00:00Zoai:scielo:S0103-50532016001101960Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2016-11-04T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv Development of Surface Plasmon Resonance-Based Immunosensor for Detection of Brucella melitensis
title Development of Surface Plasmon Resonance-Based Immunosensor for Detection of Brucella melitensis
spellingShingle Development of Surface Plasmon Resonance-Based Immunosensor for Detection of Brucella melitensis
Saberi,Fatemeh
Brucella
surface plasmon resonance
kinetics
biosensor
title_short Development of Surface Plasmon Resonance-Based Immunosensor for Detection of Brucella melitensis
title_full Development of Surface Plasmon Resonance-Based Immunosensor for Detection of Brucella melitensis
title_fullStr Development of Surface Plasmon Resonance-Based Immunosensor for Detection of Brucella melitensis
title_full_unstemmed Development of Surface Plasmon Resonance-Based Immunosensor for Detection of Brucella melitensis
title_sort Development of Surface Plasmon Resonance-Based Immunosensor for Detection of Brucella melitensis
author Saberi,Fatemeh
author_facet Saberi,Fatemeh
Kamali,Mehdi
Taheri,Ramezan A.
Ramandi,Mahdi F.
Bagdeli,Samira
Mirnejad,Reza
author_role author
author2 Kamali,Mehdi
Taheri,Ramezan A.
Ramandi,Mahdi F.
Bagdeli,Samira
Mirnejad,Reza
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Saberi,Fatemeh
Kamali,Mehdi
Taheri,Ramezan A.
Ramandi,Mahdi F.
Bagdeli,Samira
Mirnejad,Reza
dc.subject.por.fl_str_mv Brucella
surface plasmon resonance
kinetics
biosensor
topic Brucella
surface plasmon resonance
kinetics
biosensor
description A label-free optical immunosensor was developed based on surface plasmon resonance (SPR) method for rapid and selective detection of Brucella melitensis. This immunosensor was constructed by immobilizing capture antibody on 11-mercaptoundecanoic acid modified gold disk. This fabricated immunosensor detected B. melitensis at a concentration range from 103 to 107 cell mL-1 (R2 = 0.998) and a detection limit of 100 cell mL-1. Additionally, the kinetic equilibrium dissociation constant (KD) for assessment of the interaction affinity was calculated as 1.1 × 10-9 mol L-1 that can be considered as high affinity interaction. This SPR immunosensor provided advantages in term of fast response, label free and accurate detection of B. melitensis in analytical systems.
publishDate 2016
dc.date.none.fl_str_mv 2016-11-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532016001101960
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532016001101960
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.5935/0103-5053.20160085
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Journal of the Brazilian Chemical Society v.27 n.11 2016
reponame:Journal of the Brazilian Chemical Society (Online)
instname:Sociedade Brasileira de Química (SBQ)
instacron:SBQ
instname_str Sociedade Brasileira de Química (SBQ)
instacron_str SBQ
institution SBQ
reponame_str Journal of the Brazilian Chemical Society (Online)
collection Journal of the Brazilian Chemical Society (Online)
repository.name.fl_str_mv Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)
repository.mail.fl_str_mv ||office@jbcs.sbq.org.br
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