Fast Sample Preparation Method Using Ultra-High Performance Liquid Chromatography Coupled to Tandem Mass Spectrometry for Natamycin Determination in Wine Samples

• Autor(a) principal: Bernardi,Gabrieli
• Data de Publicação: 2017
• Outros Autores: Rizzetti,Tiele M., Adaime,Martha B., Zanella,Renato, Prestes,Osmar D.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Journal of the Brazilian Chemical Society (Online)
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532017000500831
• topic: natamycin; wine; sample preparation; UHPLC-MS/MS

An easy and efficient sample preparation method was developed for the determination of natamycin residues in wine samples by solvent extraction and ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS) analysis. After choosing acetonitrile as extraction solvent, an experimental design was carried out with different amounts of C18 and primary secondary amine (PSA) sorbents in order to optimize the clean-up step. Validation results were satisfactory, with recoveries from 77 to 95% and relative standard deviation (RSD) lower than 10% for the spike levels 5, 10 and 20 µg L-1. Method limit of detection (LOD) and quantification (LOQ) were 1.5 and 5.0 µg L-1, respectively. The validated method proved to be an excellent analytical tool, with a total analysis time of 3 min. The method was applied in 10 Brazilian wine samples and no residues of natamycin were detected.