A New Method for the Determination of Creatinine in Urine Samples Based on Disposable Pipette Extraction

Detalhes bibliográficos
Autor(a) principal: Fernandes,Aline R.
Data de Publicação: 2018
Outros Autores: Souza,Paulo S. de, Oliveira,Anselmo E. de, Chaves,Andréa R.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of the Brazilian Chemical Society (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532018000400695
Resumo: A method based on disposable pipette extraction (DPX) was successfully applied to creatinine determination in urine samples analysis using liquid chromatography with ultraviolet spectrophotometric detection (DPX/LC-UV). DPX variables, number of draw/eject cycles, sample pH, and type of the desorption solvent, were employed in a factorial experimental design to optimize the sorption equilibrium and time analysis. Among the evaluated DPX variables, the highest extraction efficiency was obtained with 500 µL of urine sample mixed with 1 mL of borate solution (pH 9) with one draw/eject cycle followed by liquid desorption of 1 mL of methanol in seven draw/eject cycles. The developed DPX/LC-UV method showed a linear response from the limit of quantification of 0.317 to 3.390 g L-1 with r2 = 0.996 and inter-day precision with a coefficient of variation below 8.8%. Based on these results, the proposed method can be a useful tool for determining the creatinine levels in urine samples.
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spelling A New Method for the Determination of Creatinine in Urine Samples Based on Disposable Pipette Extractioncreatininedisposable pipette extractionurine sampleliquid chromatographyA method based on disposable pipette extraction (DPX) was successfully applied to creatinine determination in urine samples analysis using liquid chromatography with ultraviolet spectrophotometric detection (DPX/LC-UV). DPX variables, number of draw/eject cycles, sample pH, and type of the desorption solvent, were employed in a factorial experimental design to optimize the sorption equilibrium and time analysis. Among the evaluated DPX variables, the highest extraction efficiency was obtained with 500 µL of urine sample mixed with 1 mL of borate solution (pH 9) with one draw/eject cycle followed by liquid desorption of 1 mL of methanol in seven draw/eject cycles. The developed DPX/LC-UV method showed a linear response from the limit of quantification of 0.317 to 3.390 g L-1 with r2 = 0.996 and inter-day precision with a coefficient of variation below 8.8%. Based on these results, the proposed method can be a useful tool for determining the creatinine levels in urine samples.Sociedade Brasileira de Química2018-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532018000400695Journal of the Brazilian Chemical Society v.29 n.4 2018reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.21577/0103-5053.20170187info:eu-repo/semantics/openAccessFernandes,Aline R.Souza,Paulo S. deOliveira,Anselmo E. deChaves,Andréa R.eng2018-03-12T00:00:00Zoai:scielo:S0103-50532018000400695Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2018-03-12T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv A New Method for the Determination of Creatinine in Urine Samples Based on Disposable Pipette Extraction
title A New Method for the Determination of Creatinine in Urine Samples Based on Disposable Pipette Extraction
spellingShingle A New Method for the Determination of Creatinine in Urine Samples Based on Disposable Pipette Extraction
Fernandes,Aline R.
creatinine
disposable pipette extraction
urine sample
liquid chromatography
title_short A New Method for the Determination of Creatinine in Urine Samples Based on Disposable Pipette Extraction
title_full A New Method for the Determination of Creatinine in Urine Samples Based on Disposable Pipette Extraction
title_fullStr A New Method for the Determination of Creatinine in Urine Samples Based on Disposable Pipette Extraction
title_full_unstemmed A New Method for the Determination of Creatinine in Urine Samples Based on Disposable Pipette Extraction
title_sort A New Method for the Determination of Creatinine in Urine Samples Based on Disposable Pipette Extraction
author Fernandes,Aline R.
author_facet Fernandes,Aline R.
Souza,Paulo S. de
Oliveira,Anselmo E. de
Chaves,Andréa R.
author_role author
author2 Souza,Paulo S. de
Oliveira,Anselmo E. de
Chaves,Andréa R.
author2_role author
author
author
dc.contributor.author.fl_str_mv Fernandes,Aline R.
Souza,Paulo S. de
Oliveira,Anselmo E. de
Chaves,Andréa R.
dc.subject.por.fl_str_mv creatinine
disposable pipette extraction
urine sample
liquid chromatography
topic creatinine
disposable pipette extraction
urine sample
liquid chromatography
description A method based on disposable pipette extraction (DPX) was successfully applied to creatinine determination in urine samples analysis using liquid chromatography with ultraviolet spectrophotometric detection (DPX/LC-UV). DPX variables, number of draw/eject cycles, sample pH, and type of the desorption solvent, were employed in a factorial experimental design to optimize the sorption equilibrium and time analysis. Among the evaluated DPX variables, the highest extraction efficiency was obtained with 500 µL of urine sample mixed with 1 mL of borate solution (pH 9) with one draw/eject cycle followed by liquid desorption of 1 mL of methanol in seven draw/eject cycles. The developed DPX/LC-UV method showed a linear response from the limit of quantification of 0.317 to 3.390 g L-1 with r2 = 0.996 and inter-day precision with a coefficient of variation below 8.8%. Based on these results, the proposed method can be a useful tool for determining the creatinine levels in urine samples.
publishDate 2018
dc.date.none.fl_str_mv 2018-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532018000400695
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532018000400695
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.21577/0103-5053.20170187
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Journal of the Brazilian Chemical Society v.29 n.4 2018
reponame:Journal of the Brazilian Chemical Society (Online)
instname:Sociedade Brasileira de Química (SBQ)
instacron:SBQ
instname_str Sociedade Brasileira de Química (SBQ)
instacron_str SBQ
institution SBQ
reponame_str Journal of the Brazilian Chemical Society (Online)
collection Journal of the Brazilian Chemical Society (Online)
repository.name.fl_str_mv Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)
repository.mail.fl_str_mv ||office@jbcs.sbq.org.br
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