Simultaneous determination of rifampicinand isoniazid in urineand pharmaceutical formulations by multivariate visible spectrophotometry

• Autor(a) principal: Stets,Sandra
• Data de Publicação: 2013
• Outros Autores: Tavares,Talita M., Peralta-Zamora,Patricio G., Pessoa,Christiana A., Nagata,Noemi
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Journal of the Brazilian Chemical Society (Online)
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532013000700014
• topic: antibiotics; PLSR; urine; derivatization reaction

Partial least squares regression (PLSR) was used for the simultaneous quantification of rifampicin (RIF)and isoniazid (INH) by visible spectrophotometry using a simple derivatization reaction. In the presence of neocuproine, copper(II) is easily reduced by INH to a Cu(I)-neocuproine complex that shows an absorption maximum at 455 nm. Under these conditions, RIF shows an absorption maximum at 449 nm. The calibration set was established between 8and 57 mg L-1 for RIFand 1.5and 7 mg L-1 for INH. The method was applied to the determination of the drugs in urine samples (recoveries between 92and 119%)and in pharmaceutical formulations (relative error lower than 5%).