Differentiation of Toxic and Non-Toxic Leaves of Jatropha curcas L. Genotypes by Leaf Spray Mass Spectrometry
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of the Brazilian Chemical Society (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532017000801461 |
Resumo: | Jatropha curcas L. is an oil crop that has been studied as a potential source of biodiesel. A high protein pie is produced as a byproduct of the biodiesel production, which could be used as animal feed. However, the pie has toxic compounds, as phorbol esters and other toxins, which prevents the use as animal feed. For this reason, Embrapa (Brazilian Agricultural Research Corporation) has been working in genetic breeding to develop non-toxic J. curcas genotypes. To evaluate this process, a simple and fast analytical technique was employed to obtain responses in a short time. Leaf spray (LS) is a recent ambient ionization mass spectrometry technique in which the sample itself serves as support and ion source. Here, toxic and non-toxic J. curcas leaves were differentiated by LS using a linear ion trap mass spectrometer and partial least squares discriminant analysis (PLS-DA) model chemometrics. It was possible to differentiate toxic and non-toxic leaves and to identify the m/z values that contribute to discrimination between the groups. |
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Differentiation of Toxic and Non-Toxic Leaves of Jatropha curcas L. Genotypes by Leaf Spray Mass Spectrometryambient mass spectrometryPLS-DAdiscrimination analysisJatropha curcas L. is an oil crop that has been studied as a potential source of biodiesel. A high protein pie is produced as a byproduct of the biodiesel production, which could be used as animal feed. However, the pie has toxic compounds, as phorbol esters and other toxins, which prevents the use as animal feed. For this reason, Embrapa (Brazilian Agricultural Research Corporation) has been working in genetic breeding to develop non-toxic J. curcas genotypes. To evaluate this process, a simple and fast analytical technique was employed to obtain responses in a short time. Leaf spray (LS) is a recent ambient ionization mass spectrometry technique in which the sample itself serves as support and ion source. Here, toxic and non-toxic J. curcas leaves were differentiated by LS using a linear ion trap mass spectrometer and partial least squares discriminant analysis (PLS-DA) model chemometrics. It was possible to differentiate toxic and non-toxic leaves and to identify the m/z values that contribute to discrimination between the groups.Sociedade Brasileira de Química2017-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532017000801461Journal of the Brazilian Chemical Society v.28 n.8 2017reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.21577/0103-5053.20160325info:eu-repo/semantics/openAccessPereira,IgorCarvalho,Thays C. deRomão,WandersonFilgueiras,Paulo R.Laviola,Bruno G.Rodrigues,Clenilson M.Abdelnur,Patrícia V.Vaz,Boniek G.eng2017-07-18T00:00:00Zoai:scielo:S0103-50532017000801461Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2017-07-18T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false |
dc.title.none.fl_str_mv |
Differentiation of Toxic and Non-Toxic Leaves of Jatropha curcas L. Genotypes by Leaf Spray Mass Spectrometry |
title |
Differentiation of Toxic and Non-Toxic Leaves of Jatropha curcas L. Genotypes by Leaf Spray Mass Spectrometry |
spellingShingle |
Differentiation of Toxic and Non-Toxic Leaves of Jatropha curcas L. Genotypes by Leaf Spray Mass Spectrometry Pereira,Igor ambient mass spectrometry PLS-DA discrimination analysis |
title_short |
Differentiation of Toxic and Non-Toxic Leaves of Jatropha curcas L. Genotypes by Leaf Spray Mass Spectrometry |
title_full |
Differentiation of Toxic and Non-Toxic Leaves of Jatropha curcas L. Genotypes by Leaf Spray Mass Spectrometry |
title_fullStr |
Differentiation of Toxic and Non-Toxic Leaves of Jatropha curcas L. Genotypes by Leaf Spray Mass Spectrometry |
title_full_unstemmed |
Differentiation of Toxic and Non-Toxic Leaves of Jatropha curcas L. Genotypes by Leaf Spray Mass Spectrometry |
title_sort |
Differentiation of Toxic and Non-Toxic Leaves of Jatropha curcas L. Genotypes by Leaf Spray Mass Spectrometry |
author |
Pereira,Igor |
author_facet |
Pereira,Igor Carvalho,Thays C. de Romão,Wanderson Filgueiras,Paulo R. Laviola,Bruno G. Rodrigues,Clenilson M. Abdelnur,Patrícia V. Vaz,Boniek G. |
author_role |
author |
author2 |
Carvalho,Thays C. de Romão,Wanderson Filgueiras,Paulo R. Laviola,Bruno G. Rodrigues,Clenilson M. Abdelnur,Patrícia V. Vaz,Boniek G. |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Pereira,Igor Carvalho,Thays C. de Romão,Wanderson Filgueiras,Paulo R. Laviola,Bruno G. Rodrigues,Clenilson M. Abdelnur,Patrícia V. Vaz,Boniek G. |
dc.subject.por.fl_str_mv |
ambient mass spectrometry PLS-DA discrimination analysis |
topic |
ambient mass spectrometry PLS-DA discrimination analysis |
description |
Jatropha curcas L. is an oil crop that has been studied as a potential source of biodiesel. A high protein pie is produced as a byproduct of the biodiesel production, which could be used as animal feed. However, the pie has toxic compounds, as phorbol esters and other toxins, which prevents the use as animal feed. For this reason, Embrapa (Brazilian Agricultural Research Corporation) has been working in genetic breeding to develop non-toxic J. curcas genotypes. To evaluate this process, a simple and fast analytical technique was employed to obtain responses in a short time. Leaf spray (LS) is a recent ambient ionization mass spectrometry technique in which the sample itself serves as support and ion source. Here, toxic and non-toxic J. curcas leaves were differentiated by LS using a linear ion trap mass spectrometer and partial least squares discriminant analysis (PLS-DA) model chemometrics. It was possible to differentiate toxic and non-toxic leaves and to identify the m/z values that contribute to discrimination between the groups. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-08-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532017000801461 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532017000801461 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.21577/0103-5053.20160325 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
dc.source.none.fl_str_mv |
Journal of the Brazilian Chemical Society v.28 n.8 2017 reponame:Journal of the Brazilian Chemical Society (Online) instname:Sociedade Brasileira de Química (SBQ) instacron:SBQ |
instname_str |
Sociedade Brasileira de Química (SBQ) |
instacron_str |
SBQ |
institution |
SBQ |
reponame_str |
Journal of the Brazilian Chemical Society (Online) |
collection |
Journal of the Brazilian Chemical Society (Online) |
repository.name.fl_str_mv |
Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ) |
repository.mail.fl_str_mv |
||office@jbcs.sbq.org.br |
_version_ |
1750318179641458688 |