Development of MSPD method for the determination of pesticide residues in tomato by GC-MS
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of the Brazilian Chemical Society (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532006000500009 |
Resumo: | A simple and effective extraction method based on matrix solid-phase dispersion was developed to determine dimethoate, methyl-parathion, malathion, tebuconazole and cypermethrin in tomato using gas chromatography-mass spectrometry and selected ion monitoring (GC-MS, SIM). Different parameters of the method were evaluated, such as type of solid phase (C18, alumina, sílica-gel and Florisil), the amount of solid phase and eluent [dichloromethane, ethyl acetate, n-hexane and n-hexane:ethyl acetate (1:1 and 1:3, v/v)]. The best results were obtained using 2.0 g of tomato, 0.5 g of alumina as dispersant sorbent, 0.5 g of Florisil as clean-up sorbent and dichloromethane as eluting solvent. The method was validated by fortified tomato samples at different concentration levels (0.05 to 4.0 mg kg-1). Average recoveries (7 replicates) ranged from 77% to 100% with relative standard deviation between 3.7% and 12.9%. Detection and quantification limits ranged from 0.01 to 0.02 mg kg-1 and 0.03 to 0.06 mg kg-1 for the whole fruit of tomato, respectively. The proposed method was applied to analyze of these compounds in commercial tomato samples and residues of methyl-parathion, dimethoate and malathion were detected on the tomato samples at concentrations below the maximum residue levels (MRLs) established by Brazilian legislation and Codex Alimentarius. |
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Development of MSPD method for the determination of pesticide residues in tomato by GC-MSMSPDextraction methodstomatopesticidesGC-MSA simple and effective extraction method based on matrix solid-phase dispersion was developed to determine dimethoate, methyl-parathion, malathion, tebuconazole and cypermethrin in tomato using gas chromatography-mass spectrometry and selected ion monitoring (GC-MS, SIM). Different parameters of the method were evaluated, such as type of solid phase (C18, alumina, sílica-gel and Florisil), the amount of solid phase and eluent [dichloromethane, ethyl acetate, n-hexane and n-hexane:ethyl acetate (1:1 and 1:3, v/v)]. The best results were obtained using 2.0 g of tomato, 0.5 g of alumina as dispersant sorbent, 0.5 g of Florisil as clean-up sorbent and dichloromethane as eluting solvent. The method was validated by fortified tomato samples at different concentration levels (0.05 to 4.0 mg kg-1). Average recoveries (7 replicates) ranged from 77% to 100% with relative standard deviation between 3.7% and 12.9%. Detection and quantification limits ranged from 0.01 to 0.02 mg kg-1 and 0.03 to 0.06 mg kg-1 for the whole fruit of tomato, respectively. The proposed method was applied to analyze of these compounds in commercial tomato samples and residues of methyl-parathion, dimethoate and malathion were detected on the tomato samples at concentrations below the maximum residue levels (MRLs) established by Brazilian legislation and Codex Alimentarius.Sociedade Brasileira de Química2006-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532006000500009Journal of the Brazilian Chemical Society v.17 n.5 2006reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.1590/S0103-50532006000500009info:eu-repo/semantics/openAccessMenezes Filho,AdalbertoNavickiene,SandroDórea,Haroldo S.eng2006-12-01T00:00:00Zoai:scielo:S0103-50532006000500009Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2006-12-01T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false |
dc.title.none.fl_str_mv |
Development of MSPD method for the determination of pesticide residues in tomato by GC-MS |
title |
Development of MSPD method for the determination of pesticide residues in tomato by GC-MS |
spellingShingle |
Development of MSPD method for the determination of pesticide residues in tomato by GC-MS Menezes Filho,Adalberto MSPD extraction methods tomato pesticides GC-MS |
title_short |
Development of MSPD method for the determination of pesticide residues in tomato by GC-MS |
title_full |
Development of MSPD method for the determination of pesticide residues in tomato by GC-MS |
title_fullStr |
Development of MSPD method for the determination of pesticide residues in tomato by GC-MS |
title_full_unstemmed |
Development of MSPD method for the determination of pesticide residues in tomato by GC-MS |
title_sort |
Development of MSPD method for the determination of pesticide residues in tomato by GC-MS |
author |
Menezes Filho,Adalberto |
author_facet |
Menezes Filho,Adalberto Navickiene,Sandro Dórea,Haroldo S. |
author_role |
author |
author2 |
Navickiene,Sandro Dórea,Haroldo S. |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Menezes Filho,Adalberto Navickiene,Sandro Dórea,Haroldo S. |
dc.subject.por.fl_str_mv |
MSPD extraction methods tomato pesticides GC-MS |
topic |
MSPD extraction methods tomato pesticides GC-MS |
description |
A simple and effective extraction method based on matrix solid-phase dispersion was developed to determine dimethoate, methyl-parathion, malathion, tebuconazole and cypermethrin in tomato using gas chromatography-mass spectrometry and selected ion monitoring (GC-MS, SIM). Different parameters of the method were evaluated, such as type of solid phase (C18, alumina, sílica-gel and Florisil), the amount of solid phase and eluent [dichloromethane, ethyl acetate, n-hexane and n-hexane:ethyl acetate (1:1 and 1:3, v/v)]. The best results were obtained using 2.0 g of tomato, 0.5 g of alumina as dispersant sorbent, 0.5 g of Florisil as clean-up sorbent and dichloromethane as eluting solvent. The method was validated by fortified tomato samples at different concentration levels (0.05 to 4.0 mg kg-1). Average recoveries (7 replicates) ranged from 77% to 100% with relative standard deviation between 3.7% and 12.9%. Detection and quantification limits ranged from 0.01 to 0.02 mg kg-1 and 0.03 to 0.06 mg kg-1 for the whole fruit of tomato, respectively. The proposed method was applied to analyze of these compounds in commercial tomato samples and residues of methyl-parathion, dimethoate and malathion were detected on the tomato samples at concentrations below the maximum residue levels (MRLs) established by Brazilian legislation and Codex Alimentarius. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532006000500009 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532006000500009 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0103-50532006000500009 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
dc.source.none.fl_str_mv |
Journal of the Brazilian Chemical Society v.17 n.5 2006 reponame:Journal of the Brazilian Chemical Society (Online) instname:Sociedade Brasileira de Química (SBQ) instacron:SBQ |
instname_str |
Sociedade Brasileira de Química (SBQ) |
instacron_str |
SBQ |
institution |
SBQ |
reponame_str |
Journal of the Brazilian Chemical Society (Online) |
collection |
Journal of the Brazilian Chemical Society (Online) |
repository.name.fl_str_mv |
Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ) |
repository.mail.fl_str_mv |
||office@jbcs.sbq.org.br |
_version_ |
1750318167342710784 |