Analysis of Xanthine Oxidase Inhibitors from Puerariae flos Using Centrifugal Ultrafiltration Coupled with HPLC-MS
Autor(a) principal: | |
---|---|
Data de Publicação: | 2017 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of the Brazilian Chemical Society (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532017000200360 |
Resumo: | In this study, centrifugal ultrafiltration coupled with high performance liquid chromatography-mass spectrometry was utilized to screen and identify xanthine oxidase inhibitors from Puerariae flos extract. The experimental conditions of centrifugal ultrafiltration including xanthine oxidase concentration, incubation time, pH and temperature were optimized. At the optimum condition (xanthine oxidase concentration: 30.0 μg mL-1, incubation time: 20 min, pH 7.0 and temperature: 25 ºC), four compounds were successfully screened from P. flos extract and identified as tectoridin, daidzin, ononin and biochanin A. The yields of tectoridin, daidzin, ononin and biochanin A were 0.231, 0.117, 0.303 and 0.089 g from 50.0 g crude P. flos samples. The inhibitory activities of these compounds were verified by xanthine oxidase inhibition assays. The experimental half maximal inhibitory concentration (IC50) values of tectoridin, daidzin, ononin and biochanin A were 88.5, 85.1, 88.8 and 87.0 μmol L-1, and the binding degree of them were 5.70, 8.28, 6.31 and 37.83% at the optimum condition, respectively. The proposed method provided a rapid and effective way to screen and analyze active compounds from natural products. |
id |
SBQ-2_4db4db7bb7614097f1cb9b1d97c11b2c |
---|---|
oai_identifier_str |
oai:scielo:S0103-50532017000200360 |
network_acronym_str |
SBQ-2 |
network_name_str |
Journal of the Brazilian Chemical Society (Online) |
repository_id_str |
|
spelling |
Analysis of Xanthine Oxidase Inhibitors from Puerariae flos Using Centrifugal Ultrafiltration Coupled with HPLC-MSHPLC-MSinhibitorPuerariae flosultrafiltrationxanthine oxidaseIn this study, centrifugal ultrafiltration coupled with high performance liquid chromatography-mass spectrometry was utilized to screen and identify xanthine oxidase inhibitors from Puerariae flos extract. The experimental conditions of centrifugal ultrafiltration including xanthine oxidase concentration, incubation time, pH and temperature were optimized. At the optimum condition (xanthine oxidase concentration: 30.0 μg mL-1, incubation time: 20 min, pH 7.0 and temperature: 25 ºC), four compounds were successfully screened from P. flos extract and identified as tectoridin, daidzin, ononin and biochanin A. The yields of tectoridin, daidzin, ononin and biochanin A were 0.231, 0.117, 0.303 and 0.089 g from 50.0 g crude P. flos samples. The inhibitory activities of these compounds were verified by xanthine oxidase inhibition assays. The experimental half maximal inhibitory concentration (IC50) values of tectoridin, daidzin, ononin and biochanin A were 88.5, 85.1, 88.8 and 87.0 μmol L-1, and the binding degree of them were 5.70, 8.28, 6.31 and 37.83% at the optimum condition, respectively. The proposed method provided a rapid and effective way to screen and analyze active compounds from natural products.Sociedade Brasileira de Química2017-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532017000200360Journal of the Brazilian Chemical Society v.28 n.2 2017reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.5935/0103-5053.20160185info:eu-repo/semantics/openAccessLiu,LiangliangXiao,AipingMa,LeiLi,Defangeng2017-01-10T00:00:00Zoai:scielo:S0103-50532017000200360Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2017-01-10T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false |
dc.title.none.fl_str_mv |
Analysis of Xanthine Oxidase Inhibitors from Puerariae flos Using Centrifugal Ultrafiltration Coupled with HPLC-MS |
title |
Analysis of Xanthine Oxidase Inhibitors from Puerariae flos Using Centrifugal Ultrafiltration Coupled with HPLC-MS |
spellingShingle |
Analysis of Xanthine Oxidase Inhibitors from Puerariae flos Using Centrifugal Ultrafiltration Coupled with HPLC-MS Liu,Liangliang HPLC-MS inhibitor Puerariae flos ultrafiltration xanthine oxidase |
title_short |
Analysis of Xanthine Oxidase Inhibitors from Puerariae flos Using Centrifugal Ultrafiltration Coupled with HPLC-MS |
title_full |
Analysis of Xanthine Oxidase Inhibitors from Puerariae flos Using Centrifugal Ultrafiltration Coupled with HPLC-MS |
title_fullStr |
Analysis of Xanthine Oxidase Inhibitors from Puerariae flos Using Centrifugal Ultrafiltration Coupled with HPLC-MS |
title_full_unstemmed |
Analysis of Xanthine Oxidase Inhibitors from Puerariae flos Using Centrifugal Ultrafiltration Coupled with HPLC-MS |
title_sort |
Analysis of Xanthine Oxidase Inhibitors from Puerariae flos Using Centrifugal Ultrafiltration Coupled with HPLC-MS |
author |
Liu,Liangliang |
author_facet |
Liu,Liangliang Xiao,Aiping Ma,Lei Li,Defang |
author_role |
author |
author2 |
Xiao,Aiping Ma,Lei Li,Defang |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Liu,Liangliang Xiao,Aiping Ma,Lei Li,Defang |
dc.subject.por.fl_str_mv |
HPLC-MS inhibitor Puerariae flos ultrafiltration xanthine oxidase |
topic |
HPLC-MS inhibitor Puerariae flos ultrafiltration xanthine oxidase |
description |
In this study, centrifugal ultrafiltration coupled with high performance liquid chromatography-mass spectrometry was utilized to screen and identify xanthine oxidase inhibitors from Puerariae flos extract. The experimental conditions of centrifugal ultrafiltration including xanthine oxidase concentration, incubation time, pH and temperature were optimized. At the optimum condition (xanthine oxidase concentration: 30.0 μg mL-1, incubation time: 20 min, pH 7.0 and temperature: 25 ºC), four compounds were successfully screened from P. flos extract and identified as tectoridin, daidzin, ononin and biochanin A. The yields of tectoridin, daidzin, ononin and biochanin A were 0.231, 0.117, 0.303 and 0.089 g from 50.0 g crude P. flos samples. The inhibitory activities of these compounds were verified by xanthine oxidase inhibition assays. The experimental half maximal inhibitory concentration (IC50) values of tectoridin, daidzin, ononin and biochanin A were 88.5, 85.1, 88.8 and 87.0 μmol L-1, and the binding degree of them were 5.70, 8.28, 6.31 and 37.83% at the optimum condition, respectively. The proposed method provided a rapid and effective way to screen and analyze active compounds from natural products. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-02-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532017000200360 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532017000200360 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.5935/0103-5053.20160185 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
dc.source.none.fl_str_mv |
Journal of the Brazilian Chemical Society v.28 n.2 2017 reponame:Journal of the Brazilian Chemical Society (Online) instname:Sociedade Brasileira de Química (SBQ) instacron:SBQ |
instname_str |
Sociedade Brasileira de Química (SBQ) |
instacron_str |
SBQ |
institution |
SBQ |
reponame_str |
Journal of the Brazilian Chemical Society (Online) |
collection |
Journal of the Brazilian Chemical Society (Online) |
repository.name.fl_str_mv |
Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ) |
repository.mail.fl_str_mv |
||office@jbcs.sbq.org.br |
_version_ |
1750318179175890944 |