Improving Yield of 1,3-Diglyceride by Whole-Cell Lipase from A. Niger GZUF36 Catalyzed Glycerolysis via Medium Optimization

• Autor(a) principal: Li,Cuiqin
• Data de Publicação: 2015
• Outros Autores: Li,Lanxiang, Zhou,Huanjing, Xia,Chaoshuang, He,Laping
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Journal of the Brazilian Chemical Society (Online)
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532015000200247
• topic: 1,3-diglyceride; screening; Aspergillus niger GZUF36; medium optimization; whole-cell lipase

1,3-Diglyceride (1,3-DG) has attracted considerable attention as a healthful food oil component. In this study, we reported a novel method to synthesis of 1,3-DG via glycerolysis of triglyceride (TG) catalyzed by whole-cell lipase from a new isolated strain, Aspergillus niger GZUF36. The glycerolysis products include major diglyceride and minor monoglyceride. To improve yield of 1,3-DG, the optimized medium for the strain was obtained by combination of single-factor experiments, Plackett-Burman (PB), the steepest ascent and Box-Behnken design (BBD). Using the optimized medium, yield of 1,3-DG synthesized by the whole-cell lipase was 26.90 mol% and 1.42-fold yield was improved. The optimized medium lays the foundation for further studies of the reaction system for synthesis of 1,3-DG. The whole-cell catalyzed-glycerolysis process represents a significant advance toward achieving economical production of 1,3-DG at industrial scale.