Piezoelectric quartz crystal sensors applied for bioanalytical assays and characterization of affinity interactions

Detalhes bibliográficos
Autor(a) principal: Skládal,Petr
Data de Publicação: 2003
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of the Brazilian Chemical Society (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532003000400002
Resumo: This review presents piezoelectric quartz crystals as transducers suitable for development of different types of bioanalytical assays. The components of measuring systems for piezosensors are described together with providers of commercial equipment. The piezoelectric biosensors are summarized for determination of viruses, bacterial and other cells, proteins, nucleic acids and small molecules as drugs, hormones and pesticides. In addition to mass changes, some agglutination assays employing viscosity effects are addressed. Finally, the direct label-free and real-time monitoring of affinity interactions using piezosensors is presented. The theoretical background for determination of appropriate kinetic rate and equilibrium constants is shown and the approach is demonstrated on the interaction of antibody with the corresponding antigen (protein secalin). Several examples of affinity studies are provided, including interactions of proteins (antibody and antigens, receptors and ligands), nucleic acids (hybridization, intercalation of metal complexes), lipids and saccharide-based layers.
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spelling Piezoelectric quartz crystal sensors applied for bioanalytical assays and characterization of affinity interactionspiezoelectric quartz crystal sensorpiezoelectric immunosensorsaffinity interactionskinetic parametersThis review presents piezoelectric quartz crystals as transducers suitable for development of different types of bioanalytical assays. The components of measuring systems for piezosensors are described together with providers of commercial equipment. The piezoelectric biosensors are summarized for determination of viruses, bacterial and other cells, proteins, nucleic acids and small molecules as drugs, hormones and pesticides. In addition to mass changes, some agglutination assays employing viscosity effects are addressed. Finally, the direct label-free and real-time monitoring of affinity interactions using piezosensors is presented. The theoretical background for determination of appropriate kinetic rate and equilibrium constants is shown and the approach is demonstrated on the interaction of antibody with the corresponding antigen (protein secalin). Several examples of affinity studies are provided, including interactions of proteins (antibody and antigens, receptors and ligands), nucleic acids (hybridization, intercalation of metal complexes), lipids and saccharide-based layers.Sociedade Brasileira de Química2003-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532003000400002Journal of the Brazilian Chemical Society v.14 n.4 2003reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.1590/S0103-50532003000400002info:eu-repo/semantics/openAccessSkládal,Petreng2003-09-15T00:00:00Zoai:scielo:S0103-50532003000400002Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2003-09-15T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv Piezoelectric quartz crystal sensors applied for bioanalytical assays and characterization of affinity interactions
title Piezoelectric quartz crystal sensors applied for bioanalytical assays and characterization of affinity interactions
spellingShingle Piezoelectric quartz crystal sensors applied for bioanalytical assays and characterization of affinity interactions
Skládal,Petr
piezoelectric quartz crystal sensor
piezoelectric immunosensors
affinity interactions
kinetic parameters
title_short Piezoelectric quartz crystal sensors applied for bioanalytical assays and characterization of affinity interactions
title_full Piezoelectric quartz crystal sensors applied for bioanalytical assays and characterization of affinity interactions
title_fullStr Piezoelectric quartz crystal sensors applied for bioanalytical assays and characterization of affinity interactions
title_full_unstemmed Piezoelectric quartz crystal sensors applied for bioanalytical assays and characterization of affinity interactions
title_sort Piezoelectric quartz crystal sensors applied for bioanalytical assays and characterization of affinity interactions
author Skládal,Petr
author_facet Skládal,Petr
author_role author
dc.contributor.author.fl_str_mv Skládal,Petr
dc.subject.por.fl_str_mv piezoelectric quartz crystal sensor
piezoelectric immunosensors
affinity interactions
kinetic parameters
topic piezoelectric quartz crystal sensor
piezoelectric immunosensors
affinity interactions
kinetic parameters
description This review presents piezoelectric quartz crystals as transducers suitable for development of different types of bioanalytical assays. The components of measuring systems for piezosensors are described together with providers of commercial equipment. The piezoelectric biosensors are summarized for determination of viruses, bacterial and other cells, proteins, nucleic acids and small molecules as drugs, hormones and pesticides. In addition to mass changes, some agglutination assays employing viscosity effects are addressed. Finally, the direct label-free and real-time monitoring of affinity interactions using piezosensors is presented. The theoretical background for determination of appropriate kinetic rate and equilibrium constants is shown and the approach is demonstrated on the interaction of antibody with the corresponding antigen (protein secalin). Several examples of affinity studies are provided, including interactions of proteins (antibody and antigens, receptors and ligands), nucleic acids (hybridization, intercalation of metal complexes), lipids and saccharide-based layers.
publishDate 2003
dc.date.none.fl_str_mv 2003-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532003000400002
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532003000400002
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0103-50532003000400002
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Journal of the Brazilian Chemical Society v.14 n.4 2003
reponame:Journal of the Brazilian Chemical Society (Online)
instname:Sociedade Brasileira de Química (SBQ)
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instname_str Sociedade Brasileira de Química (SBQ)
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reponame_str Journal of the Brazilian Chemical Society (Online)
collection Journal of the Brazilian Chemical Society (Online)
repository.name.fl_str_mv Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)
repository.mail.fl_str_mv ||office@jbcs.sbq.org.br
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