Use of Coconut Charcoal and Menthone-Thiosemicarbazone Polymer as Solid Phase Materials for the Determination of N,N-Dimethyltryptamine, Harmine, Harmaline, Harmalol, and Tetrahydroharmine in Ayahuasca Beverage by Liquid Chromatography-Tandem Mass Spectrometry

• Autor(a) principal: Navickiene,Sandro
• Data de Publicação: 2019
• Outros Autores: Santos,Luis F. S., Santos,Mônica C., Gaujac,Alain
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Journal of the Brazilian Chemical Society (Online)
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532019000100180
• topic: alkaloid; ayahuasca beverage; coconut charcoal; menthone-thiosemicarbazone; solid phase extraction

An extraction method based on solid-phase extraction was developed to determine the major alkaloid components, N,N-dimethyltryptamine, harmine, harmaline, harmalol, and tetrahydroharmine, in ayahuasca using ultra-performance liquid chromatography-tandem mass spectrometry. Coconut charcoal and menthone-thiosemicarbazone polymer were tested as new adsorbent materials. Experiments carried out at levels of 1, 5, 10, 50, 100, and 250 µg mL-1 resulted in recoveries in the ranges 45.3-115.7% for menthone-thiosemicarbazone and 40.6-116.2% for coconut charcoal. The detection and quantification limits ranged from 0.003 to 0.016 µg mL-1 and from 0.009 to 0.043 µg mL-1, respectively. The method developed was linear over the tested range (0.05-1.0 µg mL-1), with correlation coefficients ranging from 0.9969 to 0.9998. The technique was applied for analysis of the compounds in an ayahuasca sample collected from a religious group located in the city of Fortaleza, obtaining concentrations in the range from 0.3 to 19.3 mg mL-1.