Bifunctional Porous SiO2 Complex Nanoparticles with Properties of Enzymatic Catalysis and Optical Oxygen Sensing
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of the Brazilian Chemical Society (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532015000901871 |
Resumo: | The porous fluorescent SiO2 nanoparticles encapsulating photosensitizer Ru(bpy)3Cl2were prepared by Stöber method and used as a carrier to immobilize glucose oxidase (GOD) to form the bifunctional porous SiO2 complex nanoparticles (BPSCNp) with the properties of enzymatic catalysis and optical oxygen sensing. The optimal immobilization conditions are as follows: hexadecyltrimethyl ammonium bromide (APTES) concentration of 2% (v/v), glutaraldehyde (GA) concentration of 1.0% (v/v), and pH of 6.5, GOD amount of 4.0 mg in 40 mg of carrier. BPSCNp showed maximal catalytic activity at pH 6.0 and 50 °C. After immobilization, the thermal storage and operation stability of GOD were improved remarkably. After kept at 4 °C for 30 days, BPSCNp and free GOD retained 68% and 35% of initial activity, respectively. BPSCNp maintained 58% of its initial activity after 7 consecutive operations and 30% after 15 consecutive operations. The fluorescence of BPSCNp could be quenched effectively by oxygen. There was a good linear relationship between tanΦ0/tanφ and O2 concentration in the range of 0 to ca. 90%. BPSCNp had a highly reproducible response to oxygen with the response time of 20 s. BPSCNp might be used as the sensing material and have potential application in multi parameter fiber optic biosensor based on enzyme catalysis and oxygen consumption. |
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Bifunctional Porous SiO2 Complex Nanoparticles with Properties of Enzymatic Catalysis and Optical Oxygen SensingBPSCNpbifunctional propertiesenzymatic catalysisstabilityoxygen sensingThe porous fluorescent SiO2 nanoparticles encapsulating photosensitizer Ru(bpy)3Cl2were prepared by Stöber method and used as a carrier to immobilize glucose oxidase (GOD) to form the bifunctional porous SiO2 complex nanoparticles (BPSCNp) with the properties of enzymatic catalysis and optical oxygen sensing. The optimal immobilization conditions are as follows: hexadecyltrimethyl ammonium bromide (APTES) concentration of 2% (v/v), glutaraldehyde (GA) concentration of 1.0% (v/v), and pH of 6.5, GOD amount of 4.0 mg in 40 mg of carrier. BPSCNp showed maximal catalytic activity at pH 6.0 and 50 °C. After immobilization, the thermal storage and operation stability of GOD were improved remarkably. After kept at 4 °C for 30 days, BPSCNp and free GOD retained 68% and 35% of initial activity, respectively. BPSCNp maintained 58% of its initial activity after 7 consecutive operations and 30% after 15 consecutive operations. The fluorescence of BPSCNp could be quenched effectively by oxygen. There was a good linear relationship between tanΦ0/tanφ and O2 concentration in the range of 0 to ca. 90%. BPSCNp had a highly reproducible response to oxygen with the response time of 20 s. BPSCNp might be used as the sensing material and have potential application in multi parameter fiber optic biosensor based on enzyme catalysis and oxygen consumption.Sociedade Brasileira de Química2015-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532015000901871Journal of the Brazilian Chemical Society v.26 n.9 2015reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.5935/0103-5053.20150164info:eu-repo/semantics/openAccessHuang,JunLi,KunLiu,HuichaoDing,Liyuneng2015-09-11T00:00:00Zoai:scielo:S0103-50532015000901871Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2015-09-11T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false |
dc.title.none.fl_str_mv |
Bifunctional Porous SiO2 Complex Nanoparticles with Properties of Enzymatic Catalysis and Optical Oxygen Sensing |
title |
Bifunctional Porous SiO2 Complex Nanoparticles with Properties of Enzymatic Catalysis and Optical Oxygen Sensing |
spellingShingle |
Bifunctional Porous SiO2 Complex Nanoparticles with Properties of Enzymatic Catalysis and Optical Oxygen Sensing Huang,Jun BPSCNp bifunctional properties enzymatic catalysis stability oxygen sensing |
title_short |
Bifunctional Porous SiO2 Complex Nanoparticles with Properties of Enzymatic Catalysis and Optical Oxygen Sensing |
title_full |
Bifunctional Porous SiO2 Complex Nanoparticles with Properties of Enzymatic Catalysis and Optical Oxygen Sensing |
title_fullStr |
Bifunctional Porous SiO2 Complex Nanoparticles with Properties of Enzymatic Catalysis and Optical Oxygen Sensing |
title_full_unstemmed |
Bifunctional Porous SiO2 Complex Nanoparticles with Properties of Enzymatic Catalysis and Optical Oxygen Sensing |
title_sort |
Bifunctional Porous SiO2 Complex Nanoparticles with Properties of Enzymatic Catalysis and Optical Oxygen Sensing |
author |
Huang,Jun |
author_facet |
Huang,Jun Li,Kun Liu,Huichao Ding,Liyun |
author_role |
author |
author2 |
Li,Kun Liu,Huichao Ding,Liyun |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Huang,Jun Li,Kun Liu,Huichao Ding,Liyun |
dc.subject.por.fl_str_mv |
BPSCNp bifunctional properties enzymatic catalysis stability oxygen sensing |
topic |
BPSCNp bifunctional properties enzymatic catalysis stability oxygen sensing |
description |
The porous fluorescent SiO2 nanoparticles encapsulating photosensitizer Ru(bpy)3Cl2were prepared by Stöber method and used as a carrier to immobilize glucose oxidase (GOD) to form the bifunctional porous SiO2 complex nanoparticles (BPSCNp) with the properties of enzymatic catalysis and optical oxygen sensing. The optimal immobilization conditions are as follows: hexadecyltrimethyl ammonium bromide (APTES) concentration of 2% (v/v), glutaraldehyde (GA) concentration of 1.0% (v/v), and pH of 6.5, GOD amount of 4.0 mg in 40 mg of carrier. BPSCNp showed maximal catalytic activity at pH 6.0 and 50 °C. After immobilization, the thermal storage and operation stability of GOD were improved remarkably. After kept at 4 °C for 30 days, BPSCNp and free GOD retained 68% and 35% of initial activity, respectively. BPSCNp maintained 58% of its initial activity after 7 consecutive operations and 30% after 15 consecutive operations. The fluorescence of BPSCNp could be quenched effectively by oxygen. There was a good linear relationship between tanΦ0/tanφ and O2 concentration in the range of 0 to ca. 90%. BPSCNp had a highly reproducible response to oxygen with the response time of 20 s. BPSCNp might be used as the sensing material and have potential application in multi parameter fiber optic biosensor based on enzyme catalysis and oxygen consumption. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532015000901871 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532015000901871 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.5935/0103-5053.20150164 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
dc.source.none.fl_str_mv |
Journal of the Brazilian Chemical Society v.26 n.9 2015 reponame:Journal of the Brazilian Chemical Society (Online) instname:Sociedade Brasileira de Química (SBQ) instacron:SBQ |
instname_str |
Sociedade Brasileira de Química (SBQ) |
instacron_str |
SBQ |
institution |
SBQ |
reponame_str |
Journal of the Brazilian Chemical Society (Online) |
collection |
Journal of the Brazilian Chemical Society (Online) |
repository.name.fl_str_mv |
Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ) |
repository.mail.fl_str_mv |
||office@jbcs.sbq.org.br |
_version_ |
1750318177484537856 |