Biosynthetic origins of the isoprene units of 4-nerolidylcatechol in Potomorphe umbellata

• Autor(a) principal: Bergamo,Debora Cristina B.
• Data de Publicação: 2005
• Outros Autores: Kato,Massuo Jorge, Bolzani,Vanderlan da S., Furlan,Maysa
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Journal of the Brazilian Chemical Society (Online)
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532005000800018
• topic: Potomorphe umbellata; Piperaceae; 4-nerolidylcatechol; biosynthesis; sesquiterpenoid; isopentenyl diphosphate

The biosynthetic origins of the isoprene units of 4-nerolidylcatechol (1), the major constituent of Potomorphe umbellata, have been studied through feeding experiments with [14C]- and [13C]-glucose, and with precursors of the mevalonic acid and triose/pyruvate pathways, namely, [2-14C]-mevalonolactone and [U-14C]-glyceraldehyde-3-phosphate, respectively. The pattern of incorporation of label from [1-13C]-glucose into 1 was determined by quantitative 13C NMR spectroscopy. The labelling pattern revealed that the additive was specifically incorporated, and that the isoprene units of the sesquiterpenoid moiety of 4-nerolidylcatechol were derived from both the mevalonic acid and the triose/pyruvate pathways. The results indicate that both plastidic and cytoplasmic pathways are able to provide isopentenyl diphosphate units for the biosynthesis of 1.