Enantioselective analysis of omeprazole in pharmaceutical formulations by chiral high-performance liquid chromatography and capillary electrophoresis

• Autor(a) principal: Bonato,Pierina S.
• Data de Publicação: 2004
• Outros Autores: Paias,Fernanda O.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Journal of the Brazilian Chemical Society (Online)
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532004000200025
• topic: omeprazole; enantiomer; chiral HPLC; chiral CE; pharmaceutical formulation

We developed two sensitive and simple assay procedures based on high- performance liquid chromatography and capillary electrophoresis (CE) for the enantioselective analysis of omeprazole in pharmaceutical formulations. Rac-omeprazole and (S)-omeprazole were extracted from commercially available tablets using methanol:NaOH 2.5 mol L-1 (90:10, v/v). Chiral HPLC separation of omeprazole was obtained on a CHIRALPAK AD column using hexane:ethanol (40:60, v/v) as the mobile phase and detection at 302 nm. The resolution of omeprazole enantiomers by CE was carried out using 3% sulfated beta-cyclodextrin in 20 mmol L-1 phosphate buffer, pH 4.0 and detection at 202 nm.