HPLC-DAD and UV-Vis Spectrophotometric Methods for Methotrexate Assay in Different Biodegradable Microparticles

• Autor(a) principal: Oliveira,Alice R.
• Data de Publicação: 2015
• Outros Autores: Caland,Lilia B., Oliveira,Edilene G., Egito,Eryvaldo S. T., Pedrosa,Matheus F. F., Silva Júnior,Arnóbio A.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Journal of the Brazilian Chemical Society (Online)
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532015000400649
• topic: HPLC-DAD; UV-Vis spectrophotometry; validation; methotrexate; biodegradable microparticles

The challenge of the present work concerned the development and validation of high performance liquid chromatography (HPLC) and UV-Vis spectrophotometric methods for quantitation of methotrexate (MTX) loaded on biodegradable microparticles, composed of copolymers with different solubilities such as chitosan and poly (lactic-co-glycolic acid) (PLGA). The linearity of the analytical curves for MTX were > 0.999 (r2) and the limits of detection (LOD) were 0.014 µg mL-1 and 0.060 µg mL-1 for HPLC and UV-Vis spectrophotometric method, respectively. In addition, both methods were specific, robust, and accurate with a recovery between 89.5% and 105.5%. The method showed precision with relative standard deviations lower than 3.39% for HPLC and 2.90% for UV-Vis spectrophotometry. Statistical analysis revealed that both methods provide equivalent results, and also can be used for quality control of MTX-loaded in drug delivery systems.