An Ion-Pair Reagent Incorporated Polystyrene Nanofiber Applied to Solid Phase Extraction of 5-Hydroxytryptamine in Human Plasma
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of the Brazilian Chemical Society (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532015000300537 |
Resumo: | Polystyrene (PS) nanofibers incorporated with ion-pair (IP) reagent, e.g., sodium dodecyl sulfonate (SDSn), were developed as functional adsorbents for the solid-phase extraction (SPE) of ionic neurotransmitter such as 5-hydroxytryptamine (5-HT), prior to the determination by high performance liquid chromatography-fluorescence detection (HPLC-FLD). A comprehensive study was initiated to optimize the preconcentration step by exploring the main factors that affect the extraction/preconcentration efficiency of 5-HT, such as the composition of nanofibers, eluent and its volume, amount of adsorbent, pH and ionic strength. The validity of this method was investigated and optimal analytical performance was achieved including a wide dynamic range of 0.50-200 ng mL-1, detection limits of 0.50 ng mL–1 and precision (as RSD%) lower than 4% for both intra-day and inter-day assays. This method was then applied to the determination of 5-HT in human plasma with satisfactory results. |
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An Ion-Pair Reagent Incorporated Polystyrene Nanofiber Applied to Solid Phase Extraction of 5-Hydroxytryptamine in Human Plasmasolid phase extractionion-pair reagent incorporated nanofiber5-hydroxytryptaminehuman plasmaPolystyrene (PS) nanofibers incorporated with ion-pair (IP) reagent, e.g., sodium dodecyl sulfonate (SDSn), were developed as functional adsorbents for the solid-phase extraction (SPE) of ionic neurotransmitter such as 5-hydroxytryptamine (5-HT), prior to the determination by high performance liquid chromatography-fluorescence detection (HPLC-FLD). A comprehensive study was initiated to optimize the preconcentration step by exploring the main factors that affect the extraction/preconcentration efficiency of 5-HT, such as the composition of nanofibers, eluent and its volume, amount of adsorbent, pH and ionic strength. The validity of this method was investigated and optimal analytical performance was achieved including a wide dynamic range of 0.50-200 ng mL-1, detection limits of 0.50 ng mL–1 and precision (as RSD%) lower than 4% for both intra-day and inter-day assays. This method was then applied to the determination of 5-HT in human plasma with satisfactory results.Sociedade Brasileira de Química2015-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532015000300537Journal of the Brazilian Chemical Society v.26 n.3 2015reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.5935/0103-5053.20150007info:eu-repo/semantics/openAccessWang,YuZhou,XiaolingMa,YuqinKang,Xuejuneng2017-11-09T00:00:00Zoai:scielo:S0103-50532015000300537Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2017-11-09T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false |
dc.title.none.fl_str_mv |
An Ion-Pair Reagent Incorporated Polystyrene Nanofiber Applied to Solid Phase Extraction of 5-Hydroxytryptamine in Human Plasma |
title |
An Ion-Pair Reagent Incorporated Polystyrene Nanofiber Applied to Solid Phase Extraction of 5-Hydroxytryptamine in Human Plasma |
spellingShingle |
An Ion-Pair Reagent Incorporated Polystyrene Nanofiber Applied to Solid Phase Extraction of 5-Hydroxytryptamine in Human Plasma Wang,Yu solid phase extraction ion-pair reagent incorporated nanofiber 5-hydroxytryptamine human plasma |
title_short |
An Ion-Pair Reagent Incorporated Polystyrene Nanofiber Applied to Solid Phase Extraction of 5-Hydroxytryptamine in Human Plasma |
title_full |
An Ion-Pair Reagent Incorporated Polystyrene Nanofiber Applied to Solid Phase Extraction of 5-Hydroxytryptamine in Human Plasma |
title_fullStr |
An Ion-Pair Reagent Incorporated Polystyrene Nanofiber Applied to Solid Phase Extraction of 5-Hydroxytryptamine in Human Plasma |
title_full_unstemmed |
An Ion-Pair Reagent Incorporated Polystyrene Nanofiber Applied to Solid Phase Extraction of 5-Hydroxytryptamine in Human Plasma |
title_sort |
An Ion-Pair Reagent Incorporated Polystyrene Nanofiber Applied to Solid Phase Extraction of 5-Hydroxytryptamine in Human Plasma |
author |
Wang,Yu |
author_facet |
Wang,Yu Zhou,Xiaoling Ma,Yuqin Kang,Xuejun |
author_role |
author |
author2 |
Zhou,Xiaoling Ma,Yuqin Kang,Xuejun |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Wang,Yu Zhou,Xiaoling Ma,Yuqin Kang,Xuejun |
dc.subject.por.fl_str_mv |
solid phase extraction ion-pair reagent incorporated nanofiber 5-hydroxytryptamine human plasma |
topic |
solid phase extraction ion-pair reagent incorporated nanofiber 5-hydroxytryptamine human plasma |
description |
Polystyrene (PS) nanofibers incorporated with ion-pair (IP) reagent, e.g., sodium dodecyl sulfonate (SDSn), were developed as functional adsorbents for the solid-phase extraction (SPE) of ionic neurotransmitter such as 5-hydroxytryptamine (5-HT), prior to the determination by high performance liquid chromatography-fluorescence detection (HPLC-FLD). A comprehensive study was initiated to optimize the preconcentration step by exploring the main factors that affect the extraction/preconcentration efficiency of 5-HT, such as the composition of nanofibers, eluent and its volume, amount of adsorbent, pH and ionic strength. The validity of this method was investigated and optimal analytical performance was achieved including a wide dynamic range of 0.50-200 ng mL-1, detection limits of 0.50 ng mL–1 and precision (as RSD%) lower than 4% for both intra-day and inter-day assays. This method was then applied to the determination of 5-HT in human plasma with satisfactory results. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532015000300537 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532015000300537 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.5935/0103-5053.20150007 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
dc.source.none.fl_str_mv |
Journal of the Brazilian Chemical Society v.26 n.3 2015 reponame:Journal of the Brazilian Chemical Society (Online) instname:Sociedade Brasileira de Química (SBQ) instacron:SBQ |
instname_str |
Sociedade Brasileira de Química (SBQ) |
instacron_str |
SBQ |
institution |
SBQ |
reponame_str |
Journal of the Brazilian Chemical Society (Online) |
collection |
Journal of the Brazilian Chemical Society (Online) |
repository.name.fl_str_mv |
Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ) |
repository.mail.fl_str_mv |
||office@jbcs.sbq.org.br |
_version_ |
1750318177002192896 |