Simultaneous separation of ciprofloxacin, norfloxacin and ofloxacin by micellar electrokinetic chromatography
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of the Brazilian Chemical Society (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532012001000018 |
Resumo: | This work describes the development of a simple capillary electrophoresis (CE) method for simultaneous determination of the three most frequently used fluoroquinolone derivatives: ciprofloxacin, norfloxacin and ofloxacin. Due to very similar structural and physicochemical characteristics, ciprofloxacin and norfloxacin cannot be separated by the classic method of capillary zone electrophoresis (CZE). Their separations can be solved using micellar electrokinetic chromatography (MEKC), by adding an anionic surfactant to the buffer solution. The efficiency of the separation can be enhanced through the optimization of analytical parameters. A fast reliable method was developed, and the best results were obtained using a buffer solution containing 25 mmol L-1 sodium tetraborate, 100 mmol L-1 sodium dodecyl sulphate and 5% methanol as organic modifier. |
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Journal of the Brazilian Chemical Society (Online) |
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Simultaneous separation of ciprofloxacin, norfloxacin and ofloxacin by micellar electrokinetic chromatographyfluoroquinolonesmicellar electrokinetic chromatographyciprofloxacinnorfloxacinofloxacinThis work describes the development of a simple capillary electrophoresis (CE) method for simultaneous determination of the three most frequently used fluoroquinolone derivatives: ciprofloxacin, norfloxacin and ofloxacin. Due to very similar structural and physicochemical characteristics, ciprofloxacin and norfloxacin cannot be separated by the classic method of capillary zone electrophoresis (CZE). Their separations can be solved using micellar electrokinetic chromatography (MEKC), by adding an anionic surfactant to the buffer solution. The efficiency of the separation can be enhanced through the optimization of analytical parameters. A fast reliable method was developed, and the best results were obtained using a buffer solution containing 25 mmol L-1 sodium tetraborate, 100 mmol L-1 sodium dodecyl sulphate and 5% methanol as organic modifier.Sociedade Brasileira de Química2012-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532012001000018Journal of the Brazilian Chemical Society v.23 n.10 2012reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.1590/S0103-50532012005000057info:eu-repo/semantics/openAccessHancu,GabrielRusu,AuraSimon,BrigittaBoia,GeorgianaGyéresi,Árpádeng2012-12-05T00:00:00Zoai:scielo:S0103-50532012001000018Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2012-12-05T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false |
dc.title.none.fl_str_mv |
Simultaneous separation of ciprofloxacin, norfloxacin and ofloxacin by micellar electrokinetic chromatography |
title |
Simultaneous separation of ciprofloxacin, norfloxacin and ofloxacin by micellar electrokinetic chromatography |
spellingShingle |
Simultaneous separation of ciprofloxacin, norfloxacin and ofloxacin by micellar electrokinetic chromatography Hancu,Gabriel fluoroquinolones micellar electrokinetic chromatography ciprofloxacin norfloxacin ofloxacin |
title_short |
Simultaneous separation of ciprofloxacin, norfloxacin and ofloxacin by micellar electrokinetic chromatography |
title_full |
Simultaneous separation of ciprofloxacin, norfloxacin and ofloxacin by micellar electrokinetic chromatography |
title_fullStr |
Simultaneous separation of ciprofloxacin, norfloxacin and ofloxacin by micellar electrokinetic chromatography |
title_full_unstemmed |
Simultaneous separation of ciprofloxacin, norfloxacin and ofloxacin by micellar electrokinetic chromatography |
title_sort |
Simultaneous separation of ciprofloxacin, norfloxacin and ofloxacin by micellar electrokinetic chromatography |
author |
Hancu,Gabriel |
author_facet |
Hancu,Gabriel Rusu,Aura Simon,Brigitta Boia,Georgiana Gyéresi,Árpád |
author_role |
author |
author2 |
Rusu,Aura Simon,Brigitta Boia,Georgiana Gyéresi,Árpád |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Hancu,Gabriel Rusu,Aura Simon,Brigitta Boia,Georgiana Gyéresi,Árpád |
dc.subject.por.fl_str_mv |
fluoroquinolones micellar electrokinetic chromatography ciprofloxacin norfloxacin ofloxacin |
topic |
fluoroquinolones micellar electrokinetic chromatography ciprofloxacin norfloxacin ofloxacin |
description |
This work describes the development of a simple capillary electrophoresis (CE) method for simultaneous determination of the three most frequently used fluoroquinolone derivatives: ciprofloxacin, norfloxacin and ofloxacin. Due to very similar structural and physicochemical characteristics, ciprofloxacin and norfloxacin cannot be separated by the classic method of capillary zone electrophoresis (CZE). Their separations can be solved using micellar electrokinetic chromatography (MEKC), by adding an anionic surfactant to the buffer solution. The efficiency of the separation can be enhanced through the optimization of analytical parameters. A fast reliable method was developed, and the best results were obtained using a buffer solution containing 25 mmol L-1 sodium tetraborate, 100 mmol L-1 sodium dodecyl sulphate and 5% methanol as organic modifier. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532012001000018 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532012001000018 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0103-50532012005000057 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
dc.source.none.fl_str_mv |
Journal of the Brazilian Chemical Society v.23 n.10 2012 reponame:Journal of the Brazilian Chemical Society (Online) instname:Sociedade Brasileira de Química (SBQ) instacron:SBQ |
instname_str |
Sociedade Brasileira de Química (SBQ) |
instacron_str |
SBQ |
institution |
SBQ |
reponame_str |
Journal of the Brazilian Chemical Society (Online) |
collection |
Journal of the Brazilian Chemical Society (Online) |
repository.name.fl_str_mv |
Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ) |
repository.mail.fl_str_mv |
||office@jbcs.sbq.org.br |
_version_ |
1750318174381801472 |