Copaiba Oil and Its Constituent Copalic Acid as Chemotherapeutic Agents against Dermatophytes

Detalhes bibliográficos
Autor(a) principal: Nakamura,Marcela T.
Data de Publicação: 2017
Outros Autores: Endo,Eliana H., Sousa,João Paulo B. de, Callejon,Daniel R., Ueda-Nakamura,Tânia, Dias Filho,Benedito P., Freitas,Osvaldo de, Nakamura,Celso V., Lopes,Norberto P.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of the Brazilian Chemical Society (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532017000801377
Resumo: Copaiba oil, an oleoresin extracted from Copaifera genus, has been widely used in popular medicine for the treatment of several diseases. The aim of this study was to investigate the antifungal activity of the copaiba oil and its isolated compounds caryophyllene oxide, copalic acid and acetoxycopalic acid against Trichophyton rubrum, Trichophyton mentagrophytes and Microsporum gypseum strains, using microdilution method and microscopy techniques. It was found that the copaiba oil and the copalic acid were active against dermatophytes by minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC) tests. The MIC and MFC of copaiba oil against T. rubrum, T. mentagrophytes and M. gypseum were 125 µg mL-1 (250 µg mL-1), 500 µg mL-1 (500 µg mL-1) and 250 µg mL-1 (250 µg mL-1), respectively. For copalic acid, the MIC and MFC were 50 µg mL-1 (100 µg mL-1), 100 µg mL-1 (100 µg mL-1) and 50 µg mL-1 (100 µg mL-1), respectively. Fluorescence microscopy and scanning electronic microscopy were used to investigate inhibition on hyphal growth by compounds, copaiba oil and copalic acid, showing a strong inhibition and an irregular growth pattern. Cell wall, cytoplasmic membrane and intracellular contents were also damaged. In conclusion, copaiba oil and copalic acid showed great activity against dermatophytes, being potential compounds for the development of antifungal drugs.
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spelling Copaiba Oil and Its Constituent Copalic Acid as Chemotherapeutic Agents against DermatophytesCopaifera L.copaiba oildermatophytesantifungal activitycopalic acidCopaiba oil, an oleoresin extracted from Copaifera genus, has been widely used in popular medicine for the treatment of several diseases. The aim of this study was to investigate the antifungal activity of the copaiba oil and its isolated compounds caryophyllene oxide, copalic acid and acetoxycopalic acid against Trichophyton rubrum, Trichophyton mentagrophytes and Microsporum gypseum strains, using microdilution method and microscopy techniques. It was found that the copaiba oil and the copalic acid were active against dermatophytes by minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC) tests. The MIC and MFC of copaiba oil against T. rubrum, T. mentagrophytes and M. gypseum were 125 µg mL-1 (250 µg mL-1), 500 µg mL-1 (500 µg mL-1) and 250 µg mL-1 (250 µg mL-1), respectively. For copalic acid, the MIC and MFC were 50 µg mL-1 (100 µg mL-1), 100 µg mL-1 (100 µg mL-1) and 50 µg mL-1 (100 µg mL-1), respectively. Fluorescence microscopy and scanning electronic microscopy were used to investigate inhibition on hyphal growth by compounds, copaiba oil and copalic acid, showing a strong inhibition and an irregular growth pattern. Cell wall, cytoplasmic membrane and intracellular contents were also damaged. In conclusion, copaiba oil and copalic acid showed great activity against dermatophytes, being potential compounds for the development of antifungal drugs.Sociedade Brasileira de Química2017-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532017000801377Journal of the Brazilian Chemical Society v.28 n.8 2017reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.21577/0103-5053.20160309info:eu-repo/semantics/openAccessNakamura,Marcela T.Endo,Eliana H.Sousa,João Paulo B. deCallejon,Daniel R.Ueda-Nakamura,TâniaDias Filho,Benedito P.Freitas,Osvaldo deNakamura,Celso V.Lopes,Norberto P.eng2017-07-18T00:00:00Zoai:scielo:S0103-50532017000801377Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2017-07-18T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv Copaiba Oil and Its Constituent Copalic Acid as Chemotherapeutic Agents against Dermatophytes
title Copaiba Oil and Its Constituent Copalic Acid as Chemotherapeutic Agents against Dermatophytes
spellingShingle Copaiba Oil and Its Constituent Copalic Acid as Chemotherapeutic Agents against Dermatophytes
Nakamura,Marcela T.
Copaifera L.
copaiba oil
dermatophytes
antifungal activity
copalic acid
title_short Copaiba Oil and Its Constituent Copalic Acid as Chemotherapeutic Agents against Dermatophytes
title_full Copaiba Oil and Its Constituent Copalic Acid as Chemotherapeutic Agents against Dermatophytes
title_fullStr Copaiba Oil and Its Constituent Copalic Acid as Chemotherapeutic Agents against Dermatophytes
title_full_unstemmed Copaiba Oil and Its Constituent Copalic Acid as Chemotherapeutic Agents against Dermatophytes
title_sort Copaiba Oil and Its Constituent Copalic Acid as Chemotherapeutic Agents against Dermatophytes
author Nakamura,Marcela T.
author_facet Nakamura,Marcela T.
Endo,Eliana H.
Sousa,João Paulo B. de
Callejon,Daniel R.
Ueda-Nakamura,Tânia
Dias Filho,Benedito P.
Freitas,Osvaldo de
Nakamura,Celso V.
Lopes,Norberto P.
author_role author
author2 Endo,Eliana H.
Sousa,João Paulo B. de
Callejon,Daniel R.
Ueda-Nakamura,Tânia
Dias Filho,Benedito P.
Freitas,Osvaldo de
Nakamura,Celso V.
Lopes,Norberto P.
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Nakamura,Marcela T.
Endo,Eliana H.
Sousa,João Paulo B. de
Callejon,Daniel R.
Ueda-Nakamura,Tânia
Dias Filho,Benedito P.
Freitas,Osvaldo de
Nakamura,Celso V.
Lopes,Norberto P.
dc.subject.por.fl_str_mv Copaifera L.
copaiba oil
dermatophytes
antifungal activity
copalic acid
topic Copaifera L.
copaiba oil
dermatophytes
antifungal activity
copalic acid
description Copaiba oil, an oleoresin extracted from Copaifera genus, has been widely used in popular medicine for the treatment of several diseases. The aim of this study was to investigate the antifungal activity of the copaiba oil and its isolated compounds caryophyllene oxide, copalic acid and acetoxycopalic acid against Trichophyton rubrum, Trichophyton mentagrophytes and Microsporum gypseum strains, using microdilution method and microscopy techniques. It was found that the copaiba oil and the copalic acid were active against dermatophytes by minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC) tests. The MIC and MFC of copaiba oil against T. rubrum, T. mentagrophytes and M. gypseum were 125 µg mL-1 (250 µg mL-1), 500 µg mL-1 (500 µg mL-1) and 250 µg mL-1 (250 µg mL-1), respectively. For copalic acid, the MIC and MFC were 50 µg mL-1 (100 µg mL-1), 100 µg mL-1 (100 µg mL-1) and 50 µg mL-1 (100 µg mL-1), respectively. Fluorescence microscopy and scanning electronic microscopy were used to investigate inhibition on hyphal growth by compounds, copaiba oil and copalic acid, showing a strong inhibition and an irregular growth pattern. Cell wall, cytoplasmic membrane and intracellular contents were also damaged. In conclusion, copaiba oil and copalic acid showed great activity against dermatophytes, being potential compounds for the development of antifungal drugs.
publishDate 2017
dc.date.none.fl_str_mv 2017-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532017000801377
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532017000801377
dc.language.iso.fl_str_mv eng
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dc.relation.none.fl_str_mv 10.21577/0103-5053.20160309
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Journal of the Brazilian Chemical Society v.28 n.8 2017
reponame:Journal of the Brazilian Chemical Society (Online)
instname:Sociedade Brasileira de Química (SBQ)
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instname_str Sociedade Brasileira de Química (SBQ)
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institution SBQ
reponame_str Journal of the Brazilian Chemical Society (Online)
collection Journal of the Brazilian Chemical Society (Online)
repository.name.fl_str_mv Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)
repository.mail.fl_str_mv ||office@jbcs.sbq.org.br
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