Copaiba Oil and Its Constituent Copalic Acid as Chemotherapeutic Agents against Dermatophytes
Autor(a) principal: | |
---|---|
Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of the Brazilian Chemical Society (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532017000801377 |
Resumo: | Copaiba oil, an oleoresin extracted from Copaifera genus, has been widely used in popular medicine for the treatment of several diseases. The aim of this study was to investigate the antifungal activity of the copaiba oil and its isolated compounds caryophyllene oxide, copalic acid and acetoxycopalic acid against Trichophyton rubrum, Trichophyton mentagrophytes and Microsporum gypseum strains, using microdilution method and microscopy techniques. It was found that the copaiba oil and the copalic acid were active against dermatophytes by minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC) tests. The MIC and MFC of copaiba oil against T. rubrum, T. mentagrophytes and M. gypseum were 125 µg mL-1 (250 µg mL-1), 500 µg mL-1 (500 µg mL-1) and 250 µg mL-1 (250 µg mL-1), respectively. For copalic acid, the MIC and MFC were 50 µg mL-1 (100 µg mL-1), 100 µg mL-1 (100 µg mL-1) and 50 µg mL-1 (100 µg mL-1), respectively. Fluorescence microscopy and scanning electronic microscopy were used to investigate inhibition on hyphal growth by compounds, copaiba oil and copalic acid, showing a strong inhibition and an irregular growth pattern. Cell wall, cytoplasmic membrane and intracellular contents were also damaged. In conclusion, copaiba oil and copalic acid showed great activity against dermatophytes, being potential compounds for the development of antifungal drugs. |
id |
SBQ-2_865ea495fc82a28a8770fc79e9383a20 |
---|---|
oai_identifier_str |
oai:scielo:S0103-50532017000801377 |
network_acronym_str |
SBQ-2 |
network_name_str |
Journal of the Brazilian Chemical Society (Online) |
repository_id_str |
|
spelling |
Copaiba Oil and Its Constituent Copalic Acid as Chemotherapeutic Agents against DermatophytesCopaifera L.copaiba oildermatophytesantifungal activitycopalic acidCopaiba oil, an oleoresin extracted from Copaifera genus, has been widely used in popular medicine for the treatment of several diseases. The aim of this study was to investigate the antifungal activity of the copaiba oil and its isolated compounds caryophyllene oxide, copalic acid and acetoxycopalic acid against Trichophyton rubrum, Trichophyton mentagrophytes and Microsporum gypseum strains, using microdilution method and microscopy techniques. It was found that the copaiba oil and the copalic acid were active against dermatophytes by minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC) tests. The MIC and MFC of copaiba oil against T. rubrum, T. mentagrophytes and M. gypseum were 125 µg mL-1 (250 µg mL-1), 500 µg mL-1 (500 µg mL-1) and 250 µg mL-1 (250 µg mL-1), respectively. For copalic acid, the MIC and MFC were 50 µg mL-1 (100 µg mL-1), 100 µg mL-1 (100 µg mL-1) and 50 µg mL-1 (100 µg mL-1), respectively. Fluorescence microscopy and scanning electronic microscopy were used to investigate inhibition on hyphal growth by compounds, copaiba oil and copalic acid, showing a strong inhibition and an irregular growth pattern. Cell wall, cytoplasmic membrane and intracellular contents were also damaged. In conclusion, copaiba oil and copalic acid showed great activity against dermatophytes, being potential compounds for the development of antifungal drugs.Sociedade Brasileira de Química2017-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532017000801377Journal of the Brazilian Chemical Society v.28 n.8 2017reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.21577/0103-5053.20160309info:eu-repo/semantics/openAccessNakamura,Marcela T.Endo,Eliana H.Sousa,João Paulo B. deCallejon,Daniel R.Ueda-Nakamura,TâniaDias Filho,Benedito P.Freitas,Osvaldo deNakamura,Celso V.Lopes,Norberto P.eng2017-07-18T00:00:00Zoai:scielo:S0103-50532017000801377Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2017-07-18T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false |
dc.title.none.fl_str_mv |
Copaiba Oil and Its Constituent Copalic Acid as Chemotherapeutic Agents against Dermatophytes |
title |
Copaiba Oil and Its Constituent Copalic Acid as Chemotherapeutic Agents against Dermatophytes |
spellingShingle |
Copaiba Oil and Its Constituent Copalic Acid as Chemotherapeutic Agents against Dermatophytes Nakamura,Marcela T. Copaifera L. copaiba oil dermatophytes antifungal activity copalic acid |
title_short |
Copaiba Oil and Its Constituent Copalic Acid as Chemotherapeutic Agents against Dermatophytes |
title_full |
Copaiba Oil and Its Constituent Copalic Acid as Chemotherapeutic Agents against Dermatophytes |
title_fullStr |
Copaiba Oil and Its Constituent Copalic Acid as Chemotherapeutic Agents against Dermatophytes |
title_full_unstemmed |
Copaiba Oil and Its Constituent Copalic Acid as Chemotherapeutic Agents against Dermatophytes |
title_sort |
Copaiba Oil and Its Constituent Copalic Acid as Chemotherapeutic Agents against Dermatophytes |
author |
Nakamura,Marcela T. |
author_facet |
Nakamura,Marcela T. Endo,Eliana H. Sousa,João Paulo B. de Callejon,Daniel R. Ueda-Nakamura,Tânia Dias Filho,Benedito P. Freitas,Osvaldo de Nakamura,Celso V. Lopes,Norberto P. |
author_role |
author |
author2 |
Endo,Eliana H. Sousa,João Paulo B. de Callejon,Daniel R. Ueda-Nakamura,Tânia Dias Filho,Benedito P. Freitas,Osvaldo de Nakamura,Celso V. Lopes,Norberto P. |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Nakamura,Marcela T. Endo,Eliana H. Sousa,João Paulo B. de Callejon,Daniel R. Ueda-Nakamura,Tânia Dias Filho,Benedito P. Freitas,Osvaldo de Nakamura,Celso V. Lopes,Norberto P. |
dc.subject.por.fl_str_mv |
Copaifera L. copaiba oil dermatophytes antifungal activity copalic acid |
topic |
Copaifera L. copaiba oil dermatophytes antifungal activity copalic acid |
description |
Copaiba oil, an oleoresin extracted from Copaifera genus, has been widely used in popular medicine for the treatment of several diseases. The aim of this study was to investigate the antifungal activity of the copaiba oil and its isolated compounds caryophyllene oxide, copalic acid and acetoxycopalic acid against Trichophyton rubrum, Trichophyton mentagrophytes and Microsporum gypseum strains, using microdilution method and microscopy techniques. It was found that the copaiba oil and the copalic acid were active against dermatophytes by minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC) tests. The MIC and MFC of copaiba oil against T. rubrum, T. mentagrophytes and M. gypseum were 125 µg mL-1 (250 µg mL-1), 500 µg mL-1 (500 µg mL-1) and 250 µg mL-1 (250 µg mL-1), respectively. For copalic acid, the MIC and MFC were 50 µg mL-1 (100 µg mL-1), 100 µg mL-1 (100 µg mL-1) and 50 µg mL-1 (100 µg mL-1), respectively. Fluorescence microscopy and scanning electronic microscopy were used to investigate inhibition on hyphal growth by compounds, copaiba oil and copalic acid, showing a strong inhibition and an irregular growth pattern. Cell wall, cytoplasmic membrane and intracellular contents were also damaged. In conclusion, copaiba oil and copalic acid showed great activity against dermatophytes, being potential compounds for the development of antifungal drugs. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-08-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532017000801377 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532017000801377 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.21577/0103-5053.20160309 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
dc.source.none.fl_str_mv |
Journal of the Brazilian Chemical Society v.28 n.8 2017 reponame:Journal of the Brazilian Chemical Society (Online) instname:Sociedade Brasileira de Química (SBQ) instacron:SBQ |
instname_str |
Sociedade Brasileira de Química (SBQ) |
instacron_str |
SBQ |
institution |
SBQ |
reponame_str |
Journal of the Brazilian Chemical Society (Online) |
collection |
Journal of the Brazilian Chemical Society (Online) |
repository.name.fl_str_mv |
Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ) |
repository.mail.fl_str_mv |
||office@jbcs.sbq.org.br |
_version_ |
1750318179626778624 |