Simultaneous Determination of Kaempferide, Kaempferol and Isorhamnetin in Rat Plasma by Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry and its Application to a Pharmacokinetic Study
Autor(a) principal: | |
---|---|
Data de Publicação: | 2018 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of the Brazilian Chemical Society (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532018000300535 |
Resumo: | A selective, rapid and sensitive method using ultra-high performance liquid chromatography was developed and used for the simultaneous determination of kaempferide, kaempferol, and isorhamnetin in rat plasma after oral administration of Sedumsarmentosum Bunge extract. A 6430 triple-quadrupole tandem mass spectrometer in multiple reaction monitoring mode, with a negative electrospray ionization source, was used for the detection. A Waters Symmetry C18 column with isocratic elution (methanol:5 mM ammonium acetate at 75:25, v/v) was used for separation at a flow rate of 0.3 mL min-1. The method was linear for all analytes over the investigated concentration range (r > 0.9900). The intra- and inter-day precisions ranged between 3.88 and 7.74% and the accuracies ranged between 88.63 and 98.74%. The mean recoveries and matrix effect were higher than 85.66 and 92.36%, respectively. All the three compounds tested were stable during storage and analyses. The method was successfully applied to a pharmacokinetic study. |
id |
SBQ-2_8d9b18e823b3b3c2e44d6df57e0f038a |
---|---|
oai_identifier_str |
oai:scielo:S0103-50532018000300535 |
network_acronym_str |
SBQ-2 |
network_name_str |
Journal of the Brazilian Chemical Society (Online) |
repository_id_str |
|
spelling |
Simultaneous Determination of Kaempferide, Kaempferol and Isorhamnetin in Rat Plasma by Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry and its Application to a Pharmacokinetic StudySedumsarmentosum BungekaempferidekaempferolisorhamnetinpharmacokineticsA selective, rapid and sensitive method using ultra-high performance liquid chromatography was developed and used for the simultaneous determination of kaempferide, kaempferol, and isorhamnetin in rat plasma after oral administration of Sedumsarmentosum Bunge extract. A 6430 triple-quadrupole tandem mass spectrometer in multiple reaction monitoring mode, with a negative electrospray ionization source, was used for the detection. A Waters Symmetry C18 column with isocratic elution (methanol:5 mM ammonium acetate at 75:25, v/v) was used for separation at a flow rate of 0.3 mL min-1. The method was linear for all analytes over the investigated concentration range (r > 0.9900). The intra- and inter-day precisions ranged between 3.88 and 7.74% and the accuracies ranged between 88.63 and 98.74%. The mean recoveries and matrix effect were higher than 85.66 and 92.36%, respectively. All the three compounds tested were stable during storage and analyses. The method was successfully applied to a pharmacokinetic study.Sociedade Brasileira de Química2018-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532018000300535Journal of the Brazilian Chemical Society v.29 n.3 2018reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.21577/0103-5053.20170166info:eu-repo/semantics/openAccessJiang,ZhitaoWang,JianchunChen,XiaofengWang,XueWang,TongfangZhu,ZhitaoPan,Jinhuoeng2018-02-28T00:00:00Zoai:scielo:S0103-50532018000300535Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2018-02-28T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false |
dc.title.none.fl_str_mv |
Simultaneous Determination of Kaempferide, Kaempferol and Isorhamnetin in Rat Plasma by Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry and its Application to a Pharmacokinetic Study |
title |
Simultaneous Determination of Kaempferide, Kaempferol and Isorhamnetin in Rat Plasma by Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry and its Application to a Pharmacokinetic Study |
spellingShingle |
Simultaneous Determination of Kaempferide, Kaempferol and Isorhamnetin in Rat Plasma by Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry and its Application to a Pharmacokinetic Study Jiang,Zhitao Sedumsarmentosum Bunge kaempferide kaempferol isorhamnetin pharmacokinetics |
title_short |
Simultaneous Determination of Kaempferide, Kaempferol and Isorhamnetin in Rat Plasma by Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry and its Application to a Pharmacokinetic Study |
title_full |
Simultaneous Determination of Kaempferide, Kaempferol and Isorhamnetin in Rat Plasma by Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry and its Application to a Pharmacokinetic Study |
title_fullStr |
Simultaneous Determination of Kaempferide, Kaempferol and Isorhamnetin in Rat Plasma by Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry and its Application to a Pharmacokinetic Study |
title_full_unstemmed |
Simultaneous Determination of Kaempferide, Kaempferol and Isorhamnetin in Rat Plasma by Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry and its Application to a Pharmacokinetic Study |
title_sort |
Simultaneous Determination of Kaempferide, Kaempferol and Isorhamnetin in Rat Plasma by Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry and its Application to a Pharmacokinetic Study |
author |
Jiang,Zhitao |
author_facet |
Jiang,Zhitao Wang,Jianchun Chen,Xiaofeng Wang,Xue Wang,Tongfang Zhu,Zhitao Pan,Jinhuo |
author_role |
author |
author2 |
Wang,Jianchun Chen,Xiaofeng Wang,Xue Wang,Tongfang Zhu,Zhitao Pan,Jinhuo |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Jiang,Zhitao Wang,Jianchun Chen,Xiaofeng Wang,Xue Wang,Tongfang Zhu,Zhitao Pan,Jinhuo |
dc.subject.por.fl_str_mv |
Sedumsarmentosum Bunge kaempferide kaempferol isorhamnetin pharmacokinetics |
topic |
Sedumsarmentosum Bunge kaempferide kaempferol isorhamnetin pharmacokinetics |
description |
A selective, rapid and sensitive method using ultra-high performance liquid chromatography was developed and used for the simultaneous determination of kaempferide, kaempferol, and isorhamnetin in rat plasma after oral administration of Sedumsarmentosum Bunge extract. A 6430 triple-quadrupole tandem mass spectrometer in multiple reaction monitoring mode, with a negative electrospray ionization source, was used for the detection. A Waters Symmetry C18 column with isocratic elution (methanol:5 mM ammonium acetate at 75:25, v/v) was used for separation at a flow rate of 0.3 mL min-1. The method was linear for all analytes over the investigated concentration range (r > 0.9900). The intra- and inter-day precisions ranged between 3.88 and 7.74% and the accuracies ranged between 88.63 and 98.74%. The mean recoveries and matrix effect were higher than 85.66 and 92.36%, respectively. All the three compounds tested were stable during storage and analyses. The method was successfully applied to a pharmacokinetic study. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532018000300535 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532018000300535 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.21577/0103-5053.20170166 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
dc.source.none.fl_str_mv |
Journal of the Brazilian Chemical Society v.29 n.3 2018 reponame:Journal of the Brazilian Chemical Society (Online) instname:Sociedade Brasileira de Química (SBQ) instacron:SBQ |
instname_str |
Sociedade Brasileira de Química (SBQ) |
instacron_str |
SBQ |
institution |
SBQ |
reponame_str |
Journal of the Brazilian Chemical Society (Online) |
collection |
Journal of the Brazilian Chemical Society (Online) |
repository.name.fl_str_mv |
Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ) |
repository.mail.fl_str_mv |
||office@jbcs.sbq.org.br |
_version_ |
1750318180444667904 |