Direct electrochemistry of hemoglobin immobilized in polyvinylalcohol and clay composite film modified carbon ionic liquid electrode

• Autor(a) principal: Hui,Ni
• Data de Publicação: 2009
• Outros Autores: Gao,Rui-fang, Li,Xiao-qing, Sun,Wei, Jiao,Kui
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Journal of the Brazilian Chemical Society (Online)
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532009000200009
• topic: hemoglobin; room temperature ionic liquid; carbon paste electrode; direct electrochemistry; 1-butyl-3-methylimidazolium hexafluorophosphate

In this paper room temperature ionic liquid 1-butyl-3-methylimidazolium hexafluoro-phosphate (BMIMPF6) was used as binder to fabricate a carbon ionic liquid electrode (CILE). Hemoglobin (Hb) was immobilized on the surface of CILE with clay and polyvinyl alcohol (PVA) composite film by layer to layer method. UV-Vis and FT-IR spectra showed that Hb in the film retained the essential features of its native structure. Electrochemical experiments indicated that a pair of well-defined quasi-reversible redox peak was obtained in pH 7.0 Britton-Robinson (B-R) buffer solution. The reduction and oxidation peak potentials were located at -0.405 V and -0.274 V (vs. SCE) with the formal potential as -0.340 V, which was contributed to the electrochemical reaction of heme Fe(III)/Fe(II) redox couples. The results suggested that the direct electron transfer of Hb in the PVA/Clay film with the CILE was accomplished. The direct electrochemical behaviors of Hb were carefully studied with the electrochemical parameters calculated. The PVA/Clay/Hb modified CILE gave excellent electrocatalytic ability to the reduction of H2O2 and the apparent Michaelis-Menten constant (K Mapp) value of Hb in the PVA/Clay film was calculated as 56.26 µmol L-1.