Bioactive Cyanopeptides Produced by Sphaerocavum brasiliense Strains (Cyanobacteria)
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of the Brazilian Chemical Society (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532015001002088 |
Resumo: | The halogenated compounds aeruginosin and cyanopeptolin (protease inhibitors) are well documented in several cyanobacteria. In this work, the presence of genetic coding for aeruginosin and cyanopeptolin in Sphaerocavum genus and their characterization by mass spectrometry and potential toxic effects were investigated. Three strains of Sphaerocavum brasiliense (CCIBt 3094, CCIBt 3096 and CCIBt 3316) isolated from different reservoirs in São Paulo State, and one (CCIBt 3179) from Rio Grande do Norte State were selected for this study. Fragments of aerA-aerB and mcnC-mcnE gene regions (aeruginosin and cyanopeptolin, respectively) were amplified by polymerase chain reaction (PCR) and sequenced. Compounds were characterized by mass spectrometry. All the studied strains presented aeruginosin and cyanopeptolin synthetase genes. Mass spectrometry showed the presence of several aeruginosin and cyanopeptolin variants. The Allium test showed that the ethanolic extract promoted toxic and cytotoxic actions in acute and chronic treatments. These naturally occurring protease inhibitors can play an important role in the physiopathology of human diseases. |
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Bioactive Cyanopeptides Produced by Sphaerocavum brasiliense Strains (Cyanobacteria)aeruginosincyanopeptolinprotease inhibitormass spectrometryAllium testcyanobacteriaSphaerocavum genusThe halogenated compounds aeruginosin and cyanopeptolin (protease inhibitors) are well documented in several cyanobacteria. In this work, the presence of genetic coding for aeruginosin and cyanopeptolin in Sphaerocavum genus and their characterization by mass spectrometry and potential toxic effects were investigated. Three strains of Sphaerocavum brasiliense (CCIBt 3094, CCIBt 3096 and CCIBt 3316) isolated from different reservoirs in São Paulo State, and one (CCIBt 3179) from Rio Grande do Norte State were selected for this study. Fragments of aerA-aerB and mcnC-mcnE gene regions (aeruginosin and cyanopeptolin, respectively) were amplified by polymerase chain reaction (PCR) and sequenced. Compounds were characterized by mass spectrometry. All the studied strains presented aeruginosin and cyanopeptolin synthetase genes. Mass spectrometry showed the presence of several aeruginosin and cyanopeptolin variants. The Allium test showed that the ethanolic extract promoted toxic and cytotoxic actions in acute and chronic treatments. These naturally occurring protease inhibitors can play an important role in the physiopathology of human diseases.Sociedade Brasileira de Química2015-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532015001002088Journal of the Brazilian Chemical Society v.26 n.10 2015reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.5935/0103-5053.20150194info:eu-repo/semantics/openAccessSilva-Stenico,Maria EstelaRigonato,JanainaLorenzi,Adriana SturionAzevedo,Maria Teresa PaivaSant’Anna,Célia LeiteMarli Fátima Fiore,Célia Leiteeng2015-10-22T00:00:00Zoai:scielo:S0103-50532015001002088Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2015-10-22T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false |
dc.title.none.fl_str_mv |
Bioactive Cyanopeptides Produced by Sphaerocavum brasiliense Strains (Cyanobacteria) |
title |
Bioactive Cyanopeptides Produced by Sphaerocavum brasiliense Strains (Cyanobacteria) |
spellingShingle |
Bioactive Cyanopeptides Produced by Sphaerocavum brasiliense Strains (Cyanobacteria) Silva-Stenico,Maria Estela aeruginosin cyanopeptolin protease inhibitor mass spectrometry Allium test cyanobacteria Sphaerocavum genus |
title_short |
Bioactive Cyanopeptides Produced by Sphaerocavum brasiliense Strains (Cyanobacteria) |
title_full |
Bioactive Cyanopeptides Produced by Sphaerocavum brasiliense Strains (Cyanobacteria) |
title_fullStr |
Bioactive Cyanopeptides Produced by Sphaerocavum brasiliense Strains (Cyanobacteria) |
title_full_unstemmed |
Bioactive Cyanopeptides Produced by Sphaerocavum brasiliense Strains (Cyanobacteria) |
title_sort |
Bioactive Cyanopeptides Produced by Sphaerocavum brasiliense Strains (Cyanobacteria) |
author |
Silva-Stenico,Maria Estela |
author_facet |
Silva-Stenico,Maria Estela Rigonato,Janaina Lorenzi,Adriana Sturion Azevedo,Maria Teresa Paiva Sant’Anna,Célia Leite Marli Fátima Fiore,Célia Leite |
author_role |
author |
author2 |
Rigonato,Janaina Lorenzi,Adriana Sturion Azevedo,Maria Teresa Paiva Sant’Anna,Célia Leite Marli Fátima Fiore,Célia Leite |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Silva-Stenico,Maria Estela Rigonato,Janaina Lorenzi,Adriana Sturion Azevedo,Maria Teresa Paiva Sant’Anna,Célia Leite Marli Fátima Fiore,Célia Leite |
dc.subject.por.fl_str_mv |
aeruginosin cyanopeptolin protease inhibitor mass spectrometry Allium test cyanobacteria Sphaerocavum genus |
topic |
aeruginosin cyanopeptolin protease inhibitor mass spectrometry Allium test cyanobacteria Sphaerocavum genus |
description |
The halogenated compounds aeruginosin and cyanopeptolin (protease inhibitors) are well documented in several cyanobacteria. In this work, the presence of genetic coding for aeruginosin and cyanopeptolin in Sphaerocavum genus and their characterization by mass spectrometry and potential toxic effects were investigated. Three strains of Sphaerocavum brasiliense (CCIBt 3094, CCIBt 3096 and CCIBt 3316) isolated from different reservoirs in São Paulo State, and one (CCIBt 3179) from Rio Grande do Norte State were selected for this study. Fragments of aerA-aerB and mcnC-mcnE gene regions (aeruginosin and cyanopeptolin, respectively) were amplified by polymerase chain reaction (PCR) and sequenced. Compounds were characterized by mass spectrometry. All the studied strains presented aeruginosin and cyanopeptolin synthetase genes. Mass spectrometry showed the presence of several aeruginosin and cyanopeptolin variants. The Allium test showed that the ethanolic extract promoted toxic and cytotoxic actions in acute and chronic treatments. These naturally occurring protease inhibitors can play an important role in the physiopathology of human diseases. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532015001002088 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532015001002088 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.5935/0103-5053.20150194 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
dc.source.none.fl_str_mv |
Journal of the Brazilian Chemical Society v.26 n.10 2015 reponame:Journal of the Brazilian Chemical Society (Online) instname:Sociedade Brasileira de Química (SBQ) instacron:SBQ |
instname_str |
Sociedade Brasileira de Química (SBQ) |
instacron_str |
SBQ |
institution |
SBQ |
reponame_str |
Journal of the Brazilian Chemical Society (Online) |
collection |
Journal of the Brazilian Chemical Society (Online) |
repository.name.fl_str_mv |
Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ) |
repository.mail.fl_str_mv |
||office@jbcs.sbq.org.br |
_version_ |
1750318177799110656 |