Boron isotope dilution in cellular fractions of coffee leaves Evaluated by Inductively Coupled Plasma Mass Spectrometry with direct injection nebulization (DIN-ICP-MS)
Autor(a) principal: | |
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Data de Publicação: | 2003 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of the Brazilian Chemical Society (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532003000200014 |
Resumo: | Enriched 10B (94.14 atom %) was supplied to coffee plantlets for three months. Then boron isotope ratios were determined in the leaf cell compartments, cell wall, nuclei and chloroplast, after a sub-cellular fractionation procedure. The isotopic measurements were performed by Inductively Coupled Plasma Mass Spectrometry (ICP-MS) provided with a direct injection nebulizer (DIN), introducing a sample volume of 50 mL. Isotopic ratios from 1.002 to 1.326 were determined with precision characterized by RSD lower than 1.5% for the enriched cell fractions with B concentrations ranging from 3.3 to 10.8 mg g-1. The detection limit (3s) was 0.5 ng B mL-1. The average enrichments in 10B atom % found in the cell walls, nuclei and chloroplasts were 46.7, 44.5 and 48.8, respectively. |
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Boron isotope dilution in cellular fractions of coffee leaves Evaluated by Inductively Coupled Plasma Mass Spectrometry with direct injection nebulization (DIN-ICP-MS)isotope dilutioninductively coupled plasma mass spectrometrybiological cell fractionationboron isotope ratiosplant analysisEnriched 10B (94.14 atom %) was supplied to coffee plantlets for three months. Then boron isotope ratios were determined in the leaf cell compartments, cell wall, nuclei and chloroplast, after a sub-cellular fractionation procedure. The isotopic measurements were performed by Inductively Coupled Plasma Mass Spectrometry (ICP-MS) provided with a direct injection nebulizer (DIN), introducing a sample volume of 50 mL. Isotopic ratios from 1.002 to 1.326 were determined with precision characterized by RSD lower than 1.5% for the enriched cell fractions with B concentrations ranging from 3.3 to 10.8 mg g-1. The detection limit (3s) was 0.5 ng B mL-1. The average enrichments in 10B atom % found in the cell walls, nuclei and chloroplasts were 46.7, 44.5 and 48.8, respectively.Sociedade Brasileira de Química2003-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532003000200014Journal of the Brazilian Chemical Society v.14 n.2 2003reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.1590/S0103-50532003000200014info:eu-repo/semantics/openAccessBellato,Ana Cláudia S.Menegário,Amauri A.Giné,Maria Fernandaeng2015-11-26T00:00:00Zoai:scielo:S0103-50532003000200014Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2015-11-26T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false |
dc.title.none.fl_str_mv |
Boron isotope dilution in cellular fractions of coffee leaves Evaluated by Inductively Coupled Plasma Mass Spectrometry with direct injection nebulization (DIN-ICP-MS) |
title |
Boron isotope dilution in cellular fractions of coffee leaves Evaluated by Inductively Coupled Plasma Mass Spectrometry with direct injection nebulization (DIN-ICP-MS) |
spellingShingle |
Boron isotope dilution in cellular fractions of coffee leaves Evaluated by Inductively Coupled Plasma Mass Spectrometry with direct injection nebulization (DIN-ICP-MS) Bellato,Ana Cláudia S. isotope dilution inductively coupled plasma mass spectrometry biological cell fractionation boron isotope ratios plant analysis |
title_short |
Boron isotope dilution in cellular fractions of coffee leaves Evaluated by Inductively Coupled Plasma Mass Spectrometry with direct injection nebulization (DIN-ICP-MS) |
title_full |
Boron isotope dilution in cellular fractions of coffee leaves Evaluated by Inductively Coupled Plasma Mass Spectrometry with direct injection nebulization (DIN-ICP-MS) |
title_fullStr |
Boron isotope dilution in cellular fractions of coffee leaves Evaluated by Inductively Coupled Plasma Mass Spectrometry with direct injection nebulization (DIN-ICP-MS) |
title_full_unstemmed |
Boron isotope dilution in cellular fractions of coffee leaves Evaluated by Inductively Coupled Plasma Mass Spectrometry with direct injection nebulization (DIN-ICP-MS) |
title_sort |
Boron isotope dilution in cellular fractions of coffee leaves Evaluated by Inductively Coupled Plasma Mass Spectrometry with direct injection nebulization (DIN-ICP-MS) |
author |
Bellato,Ana Cláudia S. |
author_facet |
Bellato,Ana Cláudia S. Menegário,Amauri A. Giné,Maria Fernanda |
author_role |
author |
author2 |
Menegário,Amauri A. Giné,Maria Fernanda |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Bellato,Ana Cláudia S. Menegário,Amauri A. Giné,Maria Fernanda |
dc.subject.por.fl_str_mv |
isotope dilution inductively coupled plasma mass spectrometry biological cell fractionation boron isotope ratios plant analysis |
topic |
isotope dilution inductively coupled plasma mass spectrometry biological cell fractionation boron isotope ratios plant analysis |
description |
Enriched 10B (94.14 atom %) was supplied to coffee plantlets for three months. Then boron isotope ratios were determined in the leaf cell compartments, cell wall, nuclei and chloroplast, after a sub-cellular fractionation procedure. The isotopic measurements were performed by Inductively Coupled Plasma Mass Spectrometry (ICP-MS) provided with a direct injection nebulizer (DIN), introducing a sample volume of 50 mL. Isotopic ratios from 1.002 to 1.326 were determined with precision characterized by RSD lower than 1.5% for the enriched cell fractions with B concentrations ranging from 3.3 to 10.8 mg g-1. The detection limit (3s) was 0.5 ng B mL-1. The average enrichments in 10B atom % found in the cell walls, nuclei and chloroplasts were 46.7, 44.5 and 48.8, respectively. |
publishDate |
2003 |
dc.date.none.fl_str_mv |
2003-04-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532003000200014 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532003000200014 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0103-50532003000200014 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
dc.source.none.fl_str_mv |
Journal of the Brazilian Chemical Society v.14 n.2 2003 reponame:Journal of the Brazilian Chemical Society (Online) instname:Sociedade Brasileira de Química (SBQ) instacron:SBQ |
instname_str |
Sociedade Brasileira de Química (SBQ) |
instacron_str |
SBQ |
institution |
SBQ |
reponame_str |
Journal of the Brazilian Chemical Society (Online) |
collection |
Journal of the Brazilian Chemical Society (Online) |
repository.name.fl_str_mv |
Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ) |
repository.mail.fl_str_mv |
||office@jbcs.sbq.org.br |
_version_ |
1750318165026406400 |