Adapting the reducing sugars method with dinitrosalicylic acid to microtiter plates and microwave heating

• Autor(a) principal: Negrulescu,Anamaria
• Data de Publicação: 2012
• Outros Autores: Patrulea,Viorica, Mincea,Manuela M., Ionascu,Cosmin, Vlad-Oros,Beatrice A., Ostafe,Vasile
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Journal of the Brazilian Chemical Society (Online)
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532012001200005
• topic: dinitrosalicylic acid (DNS); microtiter plate assay; microwave supported reaction; reducing sugars

A sound, reliable, time- and cost-efficient method for detection of reducing sugars, based on the well known dinitrosalicylic acid (DNS) colorimetric method, adapted for microtiter plates, in a modified water bath with microwave treatment is proposed. Besides the benefic influence on the environment due to the reduction of the volume of the reagents, the modified method is rapid and allows the analysis of a relatively high number of samples. It can be applied in many types of laboratories from food processing to pharmaceutical industries. The adapted method was partially validated (linearity range between 7 µg and 5 mg per well; limit of detection 3 µg per well, standard deviation around 5% for calibration curves with glucose, and a recovery higher than 90%) and applied to a series of products containing reducing sugars, such as honey, wine samples and drugs containing lactose as an excipient. The proposed method is well-suited as a high-throughput technique for determination of reducing sugars as all the analyzed samples gave the expected results according with their technical notes.