A microdigestion procedure directly performed in the autosampler cups for the determination of trace elements in biological samples by GF AAS

Detalhes bibliográficos
Autor(a) principal: Campos,Reinaldo C.
Data de Publicação: 2009
Outros Autores: Jerez Vegueria,Sergio F., Gonçalves,Rodrigo A., Wagener,Ângela L. R.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of the Brazilian Chemical Society (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532009001000023
Resumo: A microdigestion procedure for the determination of Cd and Cu in biological samples by graphite furnace atomic absorption spectrometry (GF AAS) is described. Masses up to 5 mg are directly weighed in the autosampler cup and 100 µL of the digestion solution (1:1 v/v HNO3-H2SO4 or concentrated HNO3) are added. The cups are closed and the digestion is performed, at 60 ºC overnight in a specially designed digestion block. After cooling, 900 µL of ultrapure water is added, the solution is homogenized and the cups are transferred to the autosampler tray. Since the digestion is performed in a sealed environment and the whole procedure uses only one flask, the risks of contamination and losses are minimized. Calibration was performed with external calibration curves, in the same medium as the reagents blank. The analysis of nine different standard reference materials permitted the assessment to the accuracy of the procedure. Considering a 5 mg sample mass, the limits of quantification in the original samples calculated from ten successive measurements of the blank solution (k=10) were 0.07 and 1.7 and 0.02 and 0.3 µg g-1 for Cd and Cu respectively, using the HNO3-H2SO4 mixture or concentrated HNO3 for the digestion. The procedure was used for the determination of Cu in human liver biopsy samples.
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spelling A microdigestion procedure directly performed in the autosampler cups for the determination of trace elements in biological samples by GF AASmicrodigestion proceduretrace analysisgraphite furnace atomic absorption spectrometryA microdigestion procedure for the determination of Cd and Cu in biological samples by graphite furnace atomic absorption spectrometry (GF AAS) is described. Masses up to 5 mg are directly weighed in the autosampler cup and 100 µL of the digestion solution (1:1 v/v HNO3-H2SO4 or concentrated HNO3) are added. The cups are closed and the digestion is performed, at 60 ºC overnight in a specially designed digestion block. After cooling, 900 µL of ultrapure water is added, the solution is homogenized and the cups are transferred to the autosampler tray. Since the digestion is performed in a sealed environment and the whole procedure uses only one flask, the risks of contamination and losses are minimized. Calibration was performed with external calibration curves, in the same medium as the reagents blank. The analysis of nine different standard reference materials permitted the assessment to the accuracy of the procedure. Considering a 5 mg sample mass, the limits of quantification in the original samples calculated from ten successive measurements of the blank solution (k=10) were 0.07 and 1.7 and 0.02 and 0.3 µg g-1 for Cd and Cu respectively, using the HNO3-H2SO4 mixture or concentrated HNO3 for the digestion. The procedure was used for the determination of Cu in human liver biopsy samples.Sociedade Brasileira de Química2009-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532009001000023Journal of the Brazilian Chemical Society v.20 n.10 2009reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.1590/S0103-50532009001000023info:eu-repo/semantics/openAccessCampos,Reinaldo C.Jerez Vegueria,Sergio F.Gonçalves,Rodrigo A.Wagener,Ângela L. R.eng2011-10-14T00:00:00Zoai:scielo:S0103-50532009001000023Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2011-10-14T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv A microdigestion procedure directly performed in the autosampler cups for the determination of trace elements in biological samples by GF AAS
title A microdigestion procedure directly performed in the autosampler cups for the determination of trace elements in biological samples by GF AAS
spellingShingle A microdigestion procedure directly performed in the autosampler cups for the determination of trace elements in biological samples by GF AAS
Campos,Reinaldo C.
microdigestion procedure
trace analysis
graphite furnace atomic absorption spectrometry
title_short A microdigestion procedure directly performed in the autosampler cups for the determination of trace elements in biological samples by GF AAS
title_full A microdigestion procedure directly performed in the autosampler cups for the determination of trace elements in biological samples by GF AAS
title_fullStr A microdigestion procedure directly performed in the autosampler cups for the determination of trace elements in biological samples by GF AAS
title_full_unstemmed A microdigestion procedure directly performed in the autosampler cups for the determination of trace elements in biological samples by GF AAS
title_sort A microdigestion procedure directly performed in the autosampler cups for the determination of trace elements in biological samples by GF AAS
author Campos,Reinaldo C.
author_facet Campos,Reinaldo C.
Jerez Vegueria,Sergio F.
Gonçalves,Rodrigo A.
Wagener,Ângela L. R.
author_role author
author2 Jerez Vegueria,Sergio F.
Gonçalves,Rodrigo A.
Wagener,Ângela L. R.
author2_role author
author
author
dc.contributor.author.fl_str_mv Campos,Reinaldo C.
Jerez Vegueria,Sergio F.
Gonçalves,Rodrigo A.
Wagener,Ângela L. R.
dc.subject.por.fl_str_mv microdigestion procedure
trace analysis
graphite furnace atomic absorption spectrometry
topic microdigestion procedure
trace analysis
graphite furnace atomic absorption spectrometry
description A microdigestion procedure for the determination of Cd and Cu in biological samples by graphite furnace atomic absorption spectrometry (GF AAS) is described. Masses up to 5 mg are directly weighed in the autosampler cup and 100 µL of the digestion solution (1:1 v/v HNO3-H2SO4 or concentrated HNO3) are added. The cups are closed and the digestion is performed, at 60 ºC overnight in a specially designed digestion block. After cooling, 900 µL of ultrapure water is added, the solution is homogenized and the cups are transferred to the autosampler tray. Since the digestion is performed in a sealed environment and the whole procedure uses only one flask, the risks of contamination and losses are minimized. Calibration was performed with external calibration curves, in the same medium as the reagents blank. The analysis of nine different standard reference materials permitted the assessment to the accuracy of the procedure. Considering a 5 mg sample mass, the limits of quantification in the original samples calculated from ten successive measurements of the blank solution (k=10) were 0.07 and 1.7 and 0.02 and 0.3 µg g-1 for Cd and Cu respectively, using the HNO3-H2SO4 mixture or concentrated HNO3 for the digestion. The procedure was used for the determination of Cu in human liver biopsy samples.
publishDate 2009
dc.date.none.fl_str_mv 2009-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532009001000023
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532009001000023
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0103-50532009001000023
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Journal of the Brazilian Chemical Society v.20 n.10 2009
reponame:Journal of the Brazilian Chemical Society (Online)
instname:Sociedade Brasileira de Química (SBQ)
instacron:SBQ
instname_str Sociedade Brasileira de Química (SBQ)
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reponame_str Journal of the Brazilian Chemical Society (Online)
collection Journal of the Brazilian Chemical Society (Online)
repository.name.fl_str_mv Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)
repository.mail.fl_str_mv ||office@jbcs.sbq.org.br
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