A microdigestion procedure directly performed in the autosampler cups for the determination of trace elements in biological samples by GF AAS
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of the Brazilian Chemical Society (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532009001000023 |
Resumo: | A microdigestion procedure for the determination of Cd and Cu in biological samples by graphite furnace atomic absorption spectrometry (GF AAS) is described. Masses up to 5 mg are directly weighed in the autosampler cup and 100 µL of the digestion solution (1:1 v/v HNO3-H2SO4 or concentrated HNO3) are added. The cups are closed and the digestion is performed, at 60 ºC overnight in a specially designed digestion block. After cooling, 900 µL of ultrapure water is added, the solution is homogenized and the cups are transferred to the autosampler tray. Since the digestion is performed in a sealed environment and the whole procedure uses only one flask, the risks of contamination and losses are minimized. Calibration was performed with external calibration curves, in the same medium as the reagents blank. The analysis of nine different standard reference materials permitted the assessment to the accuracy of the procedure. Considering a 5 mg sample mass, the limits of quantification in the original samples calculated from ten successive measurements of the blank solution (k=10) were 0.07 and 1.7 and 0.02 and 0.3 µg g-1 for Cd and Cu respectively, using the HNO3-H2SO4 mixture or concentrated HNO3 for the digestion. The procedure was used for the determination of Cu in human liver biopsy samples. |
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Journal of the Brazilian Chemical Society (Online) |
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A microdigestion procedure directly performed in the autosampler cups for the determination of trace elements in biological samples by GF AASmicrodigestion proceduretrace analysisgraphite furnace atomic absorption spectrometryA microdigestion procedure for the determination of Cd and Cu in biological samples by graphite furnace atomic absorption spectrometry (GF AAS) is described. Masses up to 5 mg are directly weighed in the autosampler cup and 100 µL of the digestion solution (1:1 v/v HNO3-H2SO4 or concentrated HNO3) are added. The cups are closed and the digestion is performed, at 60 ºC overnight in a specially designed digestion block. After cooling, 900 µL of ultrapure water is added, the solution is homogenized and the cups are transferred to the autosampler tray. Since the digestion is performed in a sealed environment and the whole procedure uses only one flask, the risks of contamination and losses are minimized. Calibration was performed with external calibration curves, in the same medium as the reagents blank. The analysis of nine different standard reference materials permitted the assessment to the accuracy of the procedure. Considering a 5 mg sample mass, the limits of quantification in the original samples calculated from ten successive measurements of the blank solution (k=10) were 0.07 and 1.7 and 0.02 and 0.3 µg g-1 for Cd and Cu respectively, using the HNO3-H2SO4 mixture or concentrated HNO3 for the digestion. The procedure was used for the determination of Cu in human liver biopsy samples.Sociedade Brasileira de Química2009-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532009001000023Journal of the Brazilian Chemical Society v.20 n.10 2009reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.1590/S0103-50532009001000023info:eu-repo/semantics/openAccessCampos,Reinaldo C.Jerez Vegueria,Sergio F.Gonçalves,Rodrigo A.Wagener,Ângela L. R.eng2011-10-14T00:00:00Zoai:scielo:S0103-50532009001000023Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2011-10-14T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false |
dc.title.none.fl_str_mv |
A microdigestion procedure directly performed in the autosampler cups for the determination of trace elements in biological samples by GF AAS |
title |
A microdigestion procedure directly performed in the autosampler cups for the determination of trace elements in biological samples by GF AAS |
spellingShingle |
A microdigestion procedure directly performed in the autosampler cups for the determination of trace elements in biological samples by GF AAS Campos,Reinaldo C. microdigestion procedure trace analysis graphite furnace atomic absorption spectrometry |
title_short |
A microdigestion procedure directly performed in the autosampler cups for the determination of trace elements in biological samples by GF AAS |
title_full |
A microdigestion procedure directly performed in the autosampler cups for the determination of trace elements in biological samples by GF AAS |
title_fullStr |
A microdigestion procedure directly performed in the autosampler cups for the determination of trace elements in biological samples by GF AAS |
title_full_unstemmed |
A microdigestion procedure directly performed in the autosampler cups for the determination of trace elements in biological samples by GF AAS |
title_sort |
A microdigestion procedure directly performed in the autosampler cups for the determination of trace elements in biological samples by GF AAS |
author |
Campos,Reinaldo C. |
author_facet |
Campos,Reinaldo C. Jerez Vegueria,Sergio F. Gonçalves,Rodrigo A. Wagener,Ângela L. R. |
author_role |
author |
author2 |
Jerez Vegueria,Sergio F. Gonçalves,Rodrigo A. Wagener,Ângela L. R. |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Campos,Reinaldo C. Jerez Vegueria,Sergio F. Gonçalves,Rodrigo A. Wagener,Ângela L. R. |
dc.subject.por.fl_str_mv |
microdigestion procedure trace analysis graphite furnace atomic absorption spectrometry |
topic |
microdigestion procedure trace analysis graphite furnace atomic absorption spectrometry |
description |
A microdigestion procedure for the determination of Cd and Cu in biological samples by graphite furnace atomic absorption spectrometry (GF AAS) is described. Masses up to 5 mg are directly weighed in the autosampler cup and 100 µL of the digestion solution (1:1 v/v HNO3-H2SO4 or concentrated HNO3) are added. The cups are closed and the digestion is performed, at 60 ºC overnight in a specially designed digestion block. After cooling, 900 µL of ultrapure water is added, the solution is homogenized and the cups are transferred to the autosampler tray. Since the digestion is performed in a sealed environment and the whole procedure uses only one flask, the risks of contamination and losses are minimized. Calibration was performed with external calibration curves, in the same medium as the reagents blank. The analysis of nine different standard reference materials permitted the assessment to the accuracy of the procedure. Considering a 5 mg sample mass, the limits of quantification in the original samples calculated from ten successive measurements of the blank solution (k=10) were 0.07 and 1.7 and 0.02 and 0.3 µg g-1 for Cd and Cu respectively, using the HNO3-H2SO4 mixture or concentrated HNO3 for the digestion. The procedure was used for the determination of Cu in human liver biopsy samples. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532009001000023 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532009001000023 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0103-50532009001000023 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
dc.source.none.fl_str_mv |
Journal of the Brazilian Chemical Society v.20 n.10 2009 reponame:Journal of the Brazilian Chemical Society (Online) instname:Sociedade Brasileira de Química (SBQ) instacron:SBQ |
instname_str |
Sociedade Brasileira de Química (SBQ) |
instacron_str |
SBQ |
institution |
SBQ |
reponame_str |
Journal of the Brazilian Chemical Society (Online) |
collection |
Journal of the Brazilian Chemical Society (Online) |
repository.name.fl_str_mv |
Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ) |
repository.mail.fl_str_mv |
||office@jbcs.sbq.org.br |
_version_ |
1750318170576519168 |