Determination of Boron Isotope Ratios in Tooth Enamel by Inductively Coupled Plasma Mass Spectrometry (ICP-MS) After Matrix Separation by Ion Exchange Chromatography

• Autor(a) principal: He,Maoyong
• Data de Publicação: 2015
• Outros Autores: Jin,Zhangdong, Luo,Chongguang, Deng,Li, Xiao,Jun, Zhang,Fei
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Journal of the Brazilian Chemical Society (Online)
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532015000500949
• topic: boron isotope ratios; tooth enamel; inductively coupled plasma mass spectrometry (ICP-MS); ion exchange separation

Boron isotopes in teeth has been a new proxy for dietary reconstructions and its resistance to diagenetic alteration. In this study a method using inductively coupled plasma source mass spectrometry (ICP-MS) for the measurement of boron isotope ratio in human dental enamel has been developed. Human dental enamel were digested with HNO3-H2O2 in a microwave system. Boron in solution was separated from the matrix components using Amberlite IRA-743 resin. The factors that may affect precision and accuracy in isotope ratio determination by ICP-MS, including memory effects, mass bias drift, and concentration effects, were investigated to obtain optimum conditions. Then, the 10B/11B ratios in teeth were measured. The results showed that 2% of HNO3 + 2% of NH3•H2O, selected as the diluent/rinse solution could be effective in the elimination of boron memory effect. There was no concentration effect on boron isotope ratios when the ratio of samples B concentration to standard B concentration (refers to Csample/Cstd) varied from 0.5 to 2. The result of 10B/11B ratios in tooth enamel by sex and age fluctuated over a broad range, ranged from 0.2007 to 0.2574. This method is expected to be used for boron isotope ratio analyses in archeometry, forensic identification, paleoecology, and other disciplines in the future.