Three new amides from Streptomyces sp. H7372

• Autor(a) principal: Cheenpracha,Sarot
• Data de Publicação: 2011
• Outros Autores: Borris,Robert P, Tran,Tammy T., Jee,Jap Meng, Seow,Heng Fong, Cheah,Hwen-Yee, Ho,Coy Choke, Chang,Leng Chee
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Journal of the Brazilian Chemical Society (Online)
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532011000200006
• topic: Streptomyces sp; yeast two-hybrid screen; Ras-Raf-1 interaction; amides; Cyclo(ΔAla-L-Val)

Three new amides, methyl phenatate A (1), actiphenamide (2) and actiphenol 1-β-D-glucopyranoside (3), along with thirteen known compounds, were isolated from the organic extract of a fermentation culture of Streptomyces sp. H7372. The structures were elucidated by spectroscopic methods including 1D- and 2D-NMR techniques, and MS analyses. Cycloheximide (6) and cyclo(ΔAla-L-Val) (8) gave a clear zone of inhibition of Ras-Raf-1 interaction in the yeast two-hybrid assay which showed high potency with 10 and 25 µm clear ZOIs on SD His- and inactive on SD His+ at 2.5 µg per disk, respectively.