Determination of ursolic acid and ursolic acid lactone in the leaves of Eucalyptus tereticornis by HPLC

Detalhes bibliográficos
Autor(a) principal: Maurya,Anupam
Data de Publicação: 2012
Outros Autores: Srivastava,Santosh Kumar
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of the Brazilian Chemical Society (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532012000300013
Resumo: A simple isocratic HPLC method has been developed for the simultaneous quantification of two bioactive triterpenes, ursolic acid and ursolic acid lactone in E. tereticornis leaves. Samples were analyzed on RP-18 (4.6 × 250 mm, 5 µm) column with methanol and water acidified to pH 3.5 with TFA (88:12) at 210 nm. The method was validated and applied for the simultaneous quantification of the individual triterpenes in E. tereticornis extract. The calibration curves were linear over a concentration range of 0.05-0.3 mg mL-1 (r = 0.999 and 0.998, respectively). The limits of detection and quantification were 0.190 and 0.644 µg for ursolic acid, and 0.176 and 0.587 µg for ursolic acid lactone, while the percentage recoveries were 97.32 and 96.23% for ursolic acid and ursolic acid lactone, respectively. This is the first report on the HPLC method of ursolic acid lactone with high precision and accuracy.
id SBQ-2_b34e5c481b1594dcd6b1e5ed796c8933
oai_identifier_str oai:scielo:S0103-50532012000300013
network_acronym_str SBQ-2
network_name_str Journal of the Brazilian Chemical Society (Online)
repository_id_str
spelling Determination of ursolic acid and ursolic acid lactone in the leaves of Eucalyptus tereticornis by HPLCEucalyptus tereticornisHPLCtriterpenoidsursolic acidursolic acid lactoneA simple isocratic HPLC method has been developed for the simultaneous quantification of two bioactive triterpenes, ursolic acid and ursolic acid lactone in E. tereticornis leaves. Samples were analyzed on RP-18 (4.6 × 250 mm, 5 µm) column with methanol and water acidified to pH 3.5 with TFA (88:12) at 210 nm. The method was validated and applied for the simultaneous quantification of the individual triterpenes in E. tereticornis extract. The calibration curves were linear over a concentration range of 0.05-0.3 mg mL-1 (r = 0.999 and 0.998, respectively). The limits of detection and quantification were 0.190 and 0.644 µg for ursolic acid, and 0.176 and 0.587 µg for ursolic acid lactone, while the percentage recoveries were 97.32 and 96.23% for ursolic acid and ursolic acid lactone, respectively. This is the first report on the HPLC method of ursolic acid lactone with high precision and accuracy.Sociedade Brasileira de Química2012-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532012000300013Journal of the Brazilian Chemical Society v.23 n.3 2012reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.1590/S0103-50532012000300013info:eu-repo/semantics/openAccessMaurya,AnupamSrivastava,Santosh Kumareng2012-04-04T00:00:00Zoai:scielo:S0103-50532012000300013Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2012-04-04T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv Determination of ursolic acid and ursolic acid lactone in the leaves of Eucalyptus tereticornis by HPLC
title Determination of ursolic acid and ursolic acid lactone in the leaves of Eucalyptus tereticornis by HPLC
spellingShingle Determination of ursolic acid and ursolic acid lactone in the leaves of Eucalyptus tereticornis by HPLC
Maurya,Anupam
Eucalyptus tereticornis
HPLC
triterpenoids
ursolic acid
ursolic acid lactone
title_short Determination of ursolic acid and ursolic acid lactone in the leaves of Eucalyptus tereticornis by HPLC
title_full Determination of ursolic acid and ursolic acid lactone in the leaves of Eucalyptus tereticornis by HPLC
title_fullStr Determination of ursolic acid and ursolic acid lactone in the leaves of Eucalyptus tereticornis by HPLC
title_full_unstemmed Determination of ursolic acid and ursolic acid lactone in the leaves of Eucalyptus tereticornis by HPLC
title_sort Determination of ursolic acid and ursolic acid lactone in the leaves of Eucalyptus tereticornis by HPLC
author Maurya,Anupam
author_facet Maurya,Anupam
Srivastava,Santosh Kumar
author_role author
author2 Srivastava,Santosh Kumar
author2_role author
dc.contributor.author.fl_str_mv Maurya,Anupam
Srivastava,Santosh Kumar
dc.subject.por.fl_str_mv Eucalyptus tereticornis
HPLC
triterpenoids
ursolic acid
ursolic acid lactone
topic Eucalyptus tereticornis
HPLC
triterpenoids
ursolic acid
ursolic acid lactone
description A simple isocratic HPLC method has been developed for the simultaneous quantification of two bioactive triterpenes, ursolic acid and ursolic acid lactone in E. tereticornis leaves. Samples were analyzed on RP-18 (4.6 × 250 mm, 5 µm) column with methanol and water acidified to pH 3.5 with TFA (88:12) at 210 nm. The method was validated and applied for the simultaneous quantification of the individual triterpenes in E. tereticornis extract. The calibration curves were linear over a concentration range of 0.05-0.3 mg mL-1 (r = 0.999 and 0.998, respectively). The limits of detection and quantification were 0.190 and 0.644 µg for ursolic acid, and 0.176 and 0.587 µg for ursolic acid lactone, while the percentage recoveries were 97.32 and 96.23% for ursolic acid and ursolic acid lactone, respectively. This is the first report on the HPLC method of ursolic acid lactone with high precision and accuracy.
publishDate 2012
dc.date.none.fl_str_mv 2012-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532012000300013
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532012000300013
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0103-50532012000300013
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Journal of the Brazilian Chemical Society v.23 n.3 2012
reponame:Journal of the Brazilian Chemical Society (Online)
instname:Sociedade Brasileira de Química (SBQ)
instacron:SBQ
instname_str Sociedade Brasileira de Química (SBQ)
instacron_str SBQ
institution SBQ
reponame_str Journal of the Brazilian Chemical Society (Online)
collection Journal of the Brazilian Chemical Society (Online)
repository.name.fl_str_mv Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)
repository.mail.fl_str_mv ||office@jbcs.sbq.org.br
_version_ 1750318173119315968

Registros relacionados