Molluscicidal Activity of Compounds Isolated from Euphorbia conspicua N. E. Br

• Autor(a) principal: Mata,Rosalina C. S.
• Data de Publicação: 2011
• Outros Autores: Mendonça,Dina I. M. D. de, Vieira,Liliana, Santos,Aldenir F. dos, Silva,Luciana A. da, Gaspar,Jorge F., Martins,Célia, Rueff,José, Sant'Ana,Antônio E. G.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Journal of the Brazilian Chemical Society (Online)
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532011001000008
• topic: Euphorbia conspicua; molluscicidal activity; mutagenic activity; cytotoxic activity; genotoxicity; 3β-(E)-cinnamoyleuphorbol; 20-O-acetyl-3-O-angeloylingenol

Euphorbia conspicua latex was fractionated into triterpenic and irritant fractions I and II. The triterpenic fraction afforded 15 known compounds and a new triterpene, 3β-(E)-cinnamoyleuphorbol. 20-O-Acetyl-3-O-angeloylingenol was isolated from irritant fraction II. The compounds euphol, 3β-acetoxyeupha-8,24-diene, 3β-(E)-cinnamoyleuphorbol and 20-O-Acetyl-3-O-angeloylingenol were evaluated for molluscicidal activity. 20-O-Acetyl-3-O-angeloylingenol presented LC100 value of 1 mg mL-1, equivalent to that of the standard molluscicide niclosamide. Compounds euphol, 3β-acetoxyeupha-8,24-diene and 3β-(E)-cinnamoyleuphorbol showed low molluscicidal activity. Mutagenic assays (Ames test with strains TA 98, 100 and 102) were performed with 3β-(E)-cinnamoyleuphorbol in the presence and absence of metabolic activation (S9 mix). In V79 cells, the cytotoxicity of 3β-(E)-cinnamoyleuphorbol was evaluated using the MTT assay and the genotoxicity was assessed using the cytokinesis-block micronucleus assay (CBMN) with or without S9 mix. Mutagenic or genotoxic activity was not detected, and no significant cytotoxicity was observed for 3β-(E)-cinnamoyleuphorbol at lower doses.