Ferulate Anion Intercalated into Zn/Al Layered Double Hydroxide: A Promising Intercalation Compound for Inhibition of Leishmania (L.) amazonensis

• Autor(a) principal: Sousa,Robson
• Data de Publicação: 2019
• Outros Autores: Silva,Bruno J. M. da, Dias,Amarílis A., Meneses,Carla C. F., Bentes,Beatriz A., Silva,Edilene O., Remédios,Cláudio M. R., Feio,Waldeci P., Masson,Olivier, Alves,Cláudio N., Arruda,Mara M. S. P., Lameira,Jerônimo
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Journal of the Brazilian Chemical Society (Online)
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532019000601178
• topic: ferulic acid (FERH); intercalation; LDH-FER– intercalation compound; antileishmanial activity

Ferulic acid (FERH) is known to exert leishmanicidal action against Leishmania (Leishmania) amazonensis. In this study, the ferulate anion (FER–) was intercalated into a layered double hydroxide (LDH) for FER– delivery during an antileishmania in vitro test. The LDH-FER– intercalation compound was prepared by coprecipitation and was characterized by X-ray powder diffraction, thermogravimetry, UV-Vis, infrared, and Raman spectroscopy analyses. Leishmania (L.) amazonensisin vitro assays were conducted to assess the LDH-FER– potential. LDH-FER– was found to promote a dose-dependent reduction of L. (L.) amazonensis amastigotes (intracellular form, half maximal inhibitory concentration (IC50) = 1.78 µg mL-1) without exerting a deleterious effect on the host macrophages (medium cytotoxic concentration (CC50) = 212.61 µg mL-1 and selectivity index = 119.4). LDH-FER– was found to be an effective inhibitor of the amastigote form of L. (L.) amazonensis, suggesting that LDH-FER– was able to deliver FER– inside the parasitophorous vacuoles where the pH is more acidic.