A Rational Procedure to Increase Selectivity in the Determination of an Elicitor as Salicylic Acid in Polygala Extracts Using Capillary Electrophoresis
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of the Brazilian Chemical Society (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532022000200183 |
Resumo: | Considering the economic potential of secondary metabolites, researches have been focusing several strategies in order to explore and enhance the accumulation of these metabolites in plant matrices in a broad context. One of those strategies is the development of suitable methodologies for the determination of some elicitors, such as salicylic acid (SA). Since literature presents only a few analytical methods for the quantification of SA in plants, this study developed, validated and evaluated these metabolite contents in Polygala species using capillary zone electrophoresis with ultraviolet-visible detection. The background electrolyte (BGE) consisted of 15 mmol L−1 of hydroxymethyl-aminomethane and 30 mmol L−1 of 2-hydroxy-isobutyric acid, pH = 3.9. The BGE was chosen using a rational procedure to increase selectivity and reduce migration time. The method presented rapid performance, migration time less than 0.76 min for salicylic and phthalic acid (internal standard), precision results for peak area were better than 2.9% (intra-day) and 2.8% (inter-day) and the responses for the analytes were linear (range of 1-5 mg L−1), with limits of detection and quantification of 0.2 and 0.6 mg L−1, respectively. The developed method was applied for 39 samples of plant extracts. |
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A Rational Procedure to Increase Selectivity in the Determination of an Elicitor as Salicylic Acid in Polygala Extracts Using Capillary Electrophoresissalicylic acidchemical elicitorPolygalaplant analysiscapillary zone electrophoresisConsidering the economic potential of secondary metabolites, researches have been focusing several strategies in order to explore and enhance the accumulation of these metabolites in plant matrices in a broad context. One of those strategies is the development of suitable methodologies for the determination of some elicitors, such as salicylic acid (SA). Since literature presents only a few analytical methods for the quantification of SA in plants, this study developed, validated and evaluated these metabolite contents in Polygala species using capillary zone electrophoresis with ultraviolet-visible detection. The background electrolyte (BGE) consisted of 15 mmol L−1 of hydroxymethyl-aminomethane and 30 mmol L−1 of 2-hydroxy-isobutyric acid, pH = 3.9. The BGE was chosen using a rational procedure to increase selectivity and reduce migration time. The method presented rapid performance, migration time less than 0.76 min for salicylic and phthalic acid (internal standard), precision results for peak area were better than 2.9% (intra-day) and 2.8% (inter-day) and the responses for the analytes were linear (range of 1-5 mg L−1), with limits of detection and quantification of 0.2 and 0.6 mg L−1, respectively. The developed method was applied for 39 samples of plant extracts.Sociedade Brasileira de Química2022-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532022000200183Journal of the Brazilian Chemical Society v.33 n.2 2022reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.21577/0103-5053.20210135info:eu-repo/semantics/openAccessLopes,Kheytiany H. S.Tizziani,TiagoRuani,Ana P.Vitalia,LucianoMicke,Gustavo A.eng2022-01-21T00:00:00Zoai:scielo:S0103-50532022000200183Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2022-01-21T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false |
dc.title.none.fl_str_mv |
A Rational Procedure to Increase Selectivity in the Determination of an Elicitor as Salicylic Acid in Polygala Extracts Using Capillary Electrophoresis |
title |
A Rational Procedure to Increase Selectivity in the Determination of an Elicitor as Salicylic Acid in Polygala Extracts Using Capillary Electrophoresis |
spellingShingle |
A Rational Procedure to Increase Selectivity in the Determination of an Elicitor as Salicylic Acid in Polygala Extracts Using Capillary Electrophoresis Lopes,Kheytiany H. S. salicylic acid chemical elicitor Polygala plant analysis capillary zone electrophoresis |
title_short |
A Rational Procedure to Increase Selectivity in the Determination of an Elicitor as Salicylic Acid in Polygala Extracts Using Capillary Electrophoresis |
title_full |
A Rational Procedure to Increase Selectivity in the Determination of an Elicitor as Salicylic Acid in Polygala Extracts Using Capillary Electrophoresis |
title_fullStr |
A Rational Procedure to Increase Selectivity in the Determination of an Elicitor as Salicylic Acid in Polygala Extracts Using Capillary Electrophoresis |
title_full_unstemmed |
A Rational Procedure to Increase Selectivity in the Determination of an Elicitor as Salicylic Acid in Polygala Extracts Using Capillary Electrophoresis |
title_sort |
A Rational Procedure to Increase Selectivity in the Determination of an Elicitor as Salicylic Acid in Polygala Extracts Using Capillary Electrophoresis |
author |
Lopes,Kheytiany H. S. |
author_facet |
Lopes,Kheytiany H. S. Tizziani,Tiago Ruani,Ana P. Vitalia,Luciano Micke,Gustavo A. |
author_role |
author |
author2 |
Tizziani,Tiago Ruani,Ana P. Vitalia,Luciano Micke,Gustavo A. |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Lopes,Kheytiany H. S. Tizziani,Tiago Ruani,Ana P. Vitalia,Luciano Micke,Gustavo A. |
dc.subject.por.fl_str_mv |
salicylic acid chemical elicitor Polygala plant analysis capillary zone electrophoresis |
topic |
salicylic acid chemical elicitor Polygala plant analysis capillary zone electrophoresis |
description |
Considering the economic potential of secondary metabolites, researches have been focusing several strategies in order to explore and enhance the accumulation of these metabolites in plant matrices in a broad context. One of those strategies is the development of suitable methodologies for the determination of some elicitors, such as salicylic acid (SA). Since literature presents only a few analytical methods for the quantification of SA in plants, this study developed, validated and evaluated these metabolite contents in Polygala species using capillary zone electrophoresis with ultraviolet-visible detection. The background electrolyte (BGE) consisted of 15 mmol L−1 of hydroxymethyl-aminomethane and 30 mmol L−1 of 2-hydroxy-isobutyric acid, pH = 3.9. The BGE was chosen using a rational procedure to increase selectivity and reduce migration time. The method presented rapid performance, migration time less than 0.76 min for salicylic and phthalic acid (internal standard), precision results for peak area were better than 2.9% (intra-day) and 2.8% (inter-day) and the responses for the analytes were linear (range of 1-5 mg L−1), with limits of detection and quantification of 0.2 and 0.6 mg L−1, respectively. The developed method was applied for 39 samples of plant extracts. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-02-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532022000200183 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532022000200183 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.21577/0103-5053.20210135 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
dc.source.none.fl_str_mv |
Journal of the Brazilian Chemical Society v.33 n.2 2022 reponame:Journal of the Brazilian Chemical Society (Online) instname:Sociedade Brasileira de Química (SBQ) instacron:SBQ |
instname_str |
Sociedade Brasileira de Química (SBQ) |
instacron_str |
SBQ |
institution |
SBQ |
reponame_str |
Journal of the Brazilian Chemical Society (Online) |
collection |
Journal of the Brazilian Chemical Society (Online) |
repository.name.fl_str_mv |
Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ) |
repository.mail.fl_str_mv |
||office@jbcs.sbq.org.br |
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1750318184795209728 |