Graphite-Polyurethane Composite Electrode Modified with Molecularly Imprinted Polymer for Determination of Diclofenac
Autor(a) principal: | |
---|---|
Data de Publicação: | 2022 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of the Brazilian Chemical Society (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532022000300205 |
Resumo: | A molecularly imprinted polymer (MIP) was prepared using the anti-inflammatory diclofenac (DCF) as a template. A non-imprinted polymer (NIP) was also prepared as a control. These MIP and NIP were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM) and Brunauer-Emmett-Teller (BET), revealing a higher porosity in the first. Then both were used in the modification of graphite-polyurethane composites electrodes (GPUE). Differential pulse anodic stripping voltammetry was used for DCF determination at GPUE MIP DCF containing 2.5% (m/m) of the modifier in perchloric acid, pH = 2.0, after previously optimized conditions such as 300 s of accumulation time, +0.2 V accumulation potential (vs. SCE (saturated calomel electrode)), 50 mV pulse amplitude and 10 mV s-1 scan rate. A linear dynamic range from 0.010 to 0.20 μmol L-1 and a limit of detection (LOD) of 0.99 nmol L-1 were found, using GPUE 2.5 MIP DCF. DCF was determined in commercial pharmaceutical formulations and in synthetic urine samples, with recoveries between 101 and 102% (n = 3) and 101% (n = 3), respectively. The results agreed with the reference high-performance liquid chromatography (HPLC) within 95% confidence level, according to Student’s t-test. Interference from meclofenamic and mefenamic acids, which are structurally similar to DCF, was also evaluated. Interferences could not be totally avoided, but MIPs presented a considerable ability in discriminating the voltammetric response for DFC, despite the close structural similarity with the interferents. |
id |
SBQ-2_cd2115e2e9080cb371252efbd5ae8bb8 |
---|---|
oai_identifier_str |
oai:scielo:S0103-50532022000300205 |
network_acronym_str |
SBQ-2 |
network_name_str |
Journal of the Brazilian Chemical Society (Online) |
repository_id_str |
|
spelling |
Graphite-Polyurethane Composite Electrode Modified with Molecularly Imprinted Polymer for Determination of Diclofenacelectrochemical sensordifferential pulse anodic stripping voltammetryselectivityA molecularly imprinted polymer (MIP) was prepared using the anti-inflammatory diclofenac (DCF) as a template. A non-imprinted polymer (NIP) was also prepared as a control. These MIP and NIP were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM) and Brunauer-Emmett-Teller (BET), revealing a higher porosity in the first. Then both were used in the modification of graphite-polyurethane composites electrodes (GPUE). Differential pulse anodic stripping voltammetry was used for DCF determination at GPUE MIP DCF containing 2.5% (m/m) of the modifier in perchloric acid, pH = 2.0, after previously optimized conditions such as 300 s of accumulation time, +0.2 V accumulation potential (vs. SCE (saturated calomel electrode)), 50 mV pulse amplitude and 10 mV s-1 scan rate. A linear dynamic range from 0.010 to 0.20 μmol L-1 and a limit of detection (LOD) of 0.99 nmol L-1 were found, using GPUE 2.5 MIP DCF. DCF was determined in commercial pharmaceutical formulations and in synthetic urine samples, with recoveries between 101 and 102% (n = 3) and 101% (n = 3), respectively. The results agreed with the reference high-performance liquid chromatography (HPLC) within 95% confidence level, according to Student’s t-test. Interference from meclofenamic and mefenamic acids, which are structurally similar to DCF, was also evaluated. Interferences could not be totally avoided, but MIPs presented a considerable ability in discriminating the voltammetric response for DFC, despite the close structural similarity with the interferents.Sociedade Brasileira de Química2022-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532022000300205Journal of the Brazilian Chemical Society v.33 n.3 2022reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.21577/0103-5053.20210138info:eu-repo/semantics/openAccessPereira,Abigail VCervini,PriscilaRivera,Victor A. GCavalheiro,Éder T. Geng2022-02-18T00:00:00Zoai:scielo:S0103-50532022000300205Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2022-02-18T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false |
dc.title.none.fl_str_mv |
Graphite-Polyurethane Composite Electrode Modified with Molecularly Imprinted Polymer for Determination of Diclofenac |
title |
Graphite-Polyurethane Composite Electrode Modified with Molecularly Imprinted Polymer for Determination of Diclofenac |
spellingShingle |
Graphite-Polyurethane Composite Electrode Modified with Molecularly Imprinted Polymer for Determination of Diclofenac Pereira,Abigail V electrochemical sensor differential pulse anodic stripping voltammetry selectivity |
title_short |
Graphite-Polyurethane Composite Electrode Modified with Molecularly Imprinted Polymer for Determination of Diclofenac |
title_full |
Graphite-Polyurethane Composite Electrode Modified with Molecularly Imprinted Polymer for Determination of Diclofenac |
title_fullStr |
Graphite-Polyurethane Composite Electrode Modified with Molecularly Imprinted Polymer for Determination of Diclofenac |
title_full_unstemmed |
Graphite-Polyurethane Composite Electrode Modified with Molecularly Imprinted Polymer for Determination of Diclofenac |
title_sort |
Graphite-Polyurethane Composite Electrode Modified with Molecularly Imprinted Polymer for Determination of Diclofenac |
author |
Pereira,Abigail V |
author_facet |
Pereira,Abigail V Cervini,Priscila Rivera,Victor A. G Cavalheiro,Éder T. G |
author_role |
author |
author2 |
Cervini,Priscila Rivera,Victor A. G Cavalheiro,Éder T. G |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Pereira,Abigail V Cervini,Priscila Rivera,Victor A. G Cavalheiro,Éder T. G |
dc.subject.por.fl_str_mv |
electrochemical sensor differential pulse anodic stripping voltammetry selectivity |
topic |
electrochemical sensor differential pulse anodic stripping voltammetry selectivity |
description |
A molecularly imprinted polymer (MIP) was prepared using the anti-inflammatory diclofenac (DCF) as a template. A non-imprinted polymer (NIP) was also prepared as a control. These MIP and NIP were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM) and Brunauer-Emmett-Teller (BET), revealing a higher porosity in the first. Then both were used in the modification of graphite-polyurethane composites electrodes (GPUE). Differential pulse anodic stripping voltammetry was used for DCF determination at GPUE MIP DCF containing 2.5% (m/m) of the modifier in perchloric acid, pH = 2.0, after previously optimized conditions such as 300 s of accumulation time, +0.2 V accumulation potential (vs. SCE (saturated calomel electrode)), 50 mV pulse amplitude and 10 mV s-1 scan rate. A linear dynamic range from 0.010 to 0.20 μmol L-1 and a limit of detection (LOD) of 0.99 nmol L-1 were found, using GPUE 2.5 MIP DCF. DCF was determined in commercial pharmaceutical formulations and in synthetic urine samples, with recoveries between 101 and 102% (n = 3) and 101% (n = 3), respectively. The results agreed with the reference high-performance liquid chromatography (HPLC) within 95% confidence level, according to Student’s t-test. Interference from meclofenamic and mefenamic acids, which are structurally similar to DCF, was also evaluated. Interferences could not be totally avoided, but MIPs presented a considerable ability in discriminating the voltammetric response for DFC, despite the close structural similarity with the interferents. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532022000300205 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532022000300205 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.21577/0103-5053.20210138 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
dc.source.none.fl_str_mv |
Journal of the Brazilian Chemical Society v.33 n.3 2022 reponame:Journal of the Brazilian Chemical Society (Online) instname:Sociedade Brasileira de Química (SBQ) instacron:SBQ |
instname_str |
Sociedade Brasileira de Química (SBQ) |
instacron_str |
SBQ |
institution |
SBQ |
reponame_str |
Journal of the Brazilian Chemical Society (Online) |
collection |
Journal of the Brazilian Chemical Society (Online) |
repository.name.fl_str_mv |
Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ) |
repository.mail.fl_str_mv |
||office@jbcs.sbq.org.br |
_version_ |
1750318184800452608 |