Selective Inner-Filter on the Fluorescence Response of Chlorophyll and Pheophytin Molecules Extracted from Caesalpinia echinata Leaves
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of the Brazilian Chemical Society (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532020000100162 |
Resumo: | Photophysical processes in multicomponent solutions composed by natural pigments, extracted from Caesalpinia echinata (Brazil-wood) leaves, are investigated. Beacuse of the main fluorescent molecules in these solutions are the chlorophyll a, chlorophyll b and pheophytin a, all features are discussed regarding these three pigments. It is observed that the fluorescence bands of these macrocyclic molecules, located around ca. 673 nm, are progressively quenched due to the increase of the solutions’ concentration. Due to a significant overlapping between their fluorescence and absorption Q-bands, it is concluded that the inner-filter effect causes such quenching. Through analysis of steady-state and time-resolved fluorescence, it is verified that the inner filter acts more effectively on the emission assigned to chlorophyll a. Invoking reported extinction coefficients for these pigments in their isolated forms, we can give a qualitative interpretation of such selectivity considering the effectiveness of the overlapping between the absorption and fluorescence bands. |
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Selective Inner-Filter on the Fluorescence Response of Chlorophyll and Pheophytin Molecules Extracted from Caesalpinia echinata Leavesinner-filterfluorescence spectroscopymulticomponent solutionschlorophyllsdeconvolutionPhotophysical processes in multicomponent solutions composed by natural pigments, extracted from Caesalpinia echinata (Brazil-wood) leaves, are investigated. Beacuse of the main fluorescent molecules in these solutions are the chlorophyll a, chlorophyll b and pheophytin a, all features are discussed regarding these three pigments. It is observed that the fluorescence bands of these macrocyclic molecules, located around ca. 673 nm, are progressively quenched due to the increase of the solutions’ concentration. Due to a significant overlapping between their fluorescence and absorption Q-bands, it is concluded that the inner-filter effect causes such quenching. Through analysis of steady-state and time-resolved fluorescence, it is verified that the inner filter acts more effectively on the emission assigned to chlorophyll a. Invoking reported extinction coefficients for these pigments in their isolated forms, we can give a qualitative interpretation of such selectivity considering the effectiveness of the overlapping between the absorption and fluorescence bands.Sociedade Brasileira de Química2020-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532020000100162Journal of the Brazilian Chemical Society v.31 n.1 2020reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.21577/0103-5053.20190150info:eu-repo/semantics/openAccessLopes,Jefferson M. S.Moreira,Sanclayton G. C.Barbosa Neto,Newton M.eng2020-06-05T00:00:00Zoai:scielo:S0103-50532020000100162Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2020-06-05T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false |
dc.title.none.fl_str_mv |
Selective Inner-Filter on the Fluorescence Response of Chlorophyll and Pheophytin Molecules Extracted from Caesalpinia echinata Leaves |
title |
Selective Inner-Filter on the Fluorescence Response of Chlorophyll and Pheophytin Molecules Extracted from Caesalpinia echinata Leaves |
spellingShingle |
Selective Inner-Filter on the Fluorescence Response of Chlorophyll and Pheophytin Molecules Extracted from Caesalpinia echinata Leaves Lopes,Jefferson M. S. inner-filter fluorescence spectroscopy multicomponent solutions chlorophylls deconvolution |
title_short |
Selective Inner-Filter on the Fluorescence Response of Chlorophyll and Pheophytin Molecules Extracted from Caesalpinia echinata Leaves |
title_full |
Selective Inner-Filter on the Fluorescence Response of Chlorophyll and Pheophytin Molecules Extracted from Caesalpinia echinata Leaves |
title_fullStr |
Selective Inner-Filter on the Fluorescence Response of Chlorophyll and Pheophytin Molecules Extracted from Caesalpinia echinata Leaves |
title_full_unstemmed |
Selective Inner-Filter on the Fluorescence Response of Chlorophyll and Pheophytin Molecules Extracted from Caesalpinia echinata Leaves |
title_sort |
Selective Inner-Filter on the Fluorescence Response of Chlorophyll and Pheophytin Molecules Extracted from Caesalpinia echinata Leaves |
author |
Lopes,Jefferson M. S. |
author_facet |
Lopes,Jefferson M. S. Moreira,Sanclayton G. C. Barbosa Neto,Newton M. |
author_role |
author |
author2 |
Moreira,Sanclayton G. C. Barbosa Neto,Newton M. |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Lopes,Jefferson M. S. Moreira,Sanclayton G. C. Barbosa Neto,Newton M. |
dc.subject.por.fl_str_mv |
inner-filter fluorescence spectroscopy multicomponent solutions chlorophylls deconvolution |
topic |
inner-filter fluorescence spectroscopy multicomponent solutions chlorophylls deconvolution |
description |
Photophysical processes in multicomponent solutions composed by natural pigments, extracted from Caesalpinia echinata (Brazil-wood) leaves, are investigated. Beacuse of the main fluorescent molecules in these solutions are the chlorophyll a, chlorophyll b and pheophytin a, all features are discussed regarding these three pigments. It is observed that the fluorescence bands of these macrocyclic molecules, located around ca. 673 nm, are progressively quenched due to the increase of the solutions’ concentration. Due to a significant overlapping between their fluorescence and absorption Q-bands, it is concluded that the inner-filter effect causes such quenching. Through analysis of steady-state and time-resolved fluorescence, it is verified that the inner filter acts more effectively on the emission assigned to chlorophyll a. Invoking reported extinction coefficients for these pigments in their isolated forms, we can give a qualitative interpretation of such selectivity considering the effectiveness of the overlapping between the absorption and fluorescence bands. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532020000100162 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532020000100162 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.21577/0103-5053.20190150 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
dc.source.none.fl_str_mv |
Journal of the Brazilian Chemical Society v.31 n.1 2020 reponame:Journal of the Brazilian Chemical Society (Online) instname:Sociedade Brasileira de Química (SBQ) instacron:SBQ |
instname_str |
Sociedade Brasileira de Química (SBQ) |
instacron_str |
SBQ |
institution |
SBQ |
reponame_str |
Journal of the Brazilian Chemical Society (Online) |
collection |
Journal of the Brazilian Chemical Society (Online) |
repository.name.fl_str_mv |
Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ) |
repository.mail.fl_str_mv |
||office@jbcs.sbq.org.br |
_version_ |
1750318182554402816 |