1H HR-MAS NMR and S180 cells: metabolite assignment and evaluation of pulse sequence

Detalhes bibliográficos
Autor(a) principal: Oliveira,Aline L. de
Data de Publicação: 2014
Outros Autores: Martinelli,Bruno César B., Lião,Luciano M., Pereira,Flávia C., Silveira-Lacerda,Elisangela P., Alcantara,Glaucia B.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of the Brazilian Chemical Society (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532014000600019
Resumo: High resolution magic angle spinning ¹H nuclear magnetic resonance spectroscopy (HR-MAS NMR) is a useful technique for evaluation of intact cells and tissues. However, optimal NMR parameters are crucial in obtaining reliable results. To identify the key steps for the optimization of HR-MAS NMR parameters, we assessed different pulse sequences and NMR parameters using sarcoma 180 (S180) cells. A complete assignment of the metabolites of S180 is given to assist future studies.
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spelling 1H HR-MAS NMR and S180 cells: metabolite assignment and evaluation of pulse sequenceHR-MAS NMRsarcoma 180S180CPMGHigh resolution magic angle spinning ¹H nuclear magnetic resonance spectroscopy (HR-MAS NMR) is a useful technique for evaluation of intact cells and tissues. However, optimal NMR parameters are crucial in obtaining reliable results. To identify the key steps for the optimization of HR-MAS NMR parameters, we assessed different pulse sequences and NMR parameters using sarcoma 180 (S180) cells. A complete assignment of the metabolites of S180 is given to assist future studies.Sociedade Brasileira de Química2014-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532014000600019Journal of the Brazilian Chemical Society v.25 n.6 2014reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.5935/0103-5053.20140070info:eu-repo/semantics/openAccessOliveira,Aline L. deMartinelli,Bruno César B.Lião,Luciano M.Pereira,Flávia C.Silveira-Lacerda,Elisangela P.Alcantara,Glaucia B.eng2014-07-01T00:00:00Zoai:scielo:S0103-50532014000600019Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2014-07-01T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv 1H HR-MAS NMR and S180 cells: metabolite assignment and evaluation of pulse sequence
title 1H HR-MAS NMR and S180 cells: metabolite assignment and evaluation of pulse sequence
spellingShingle 1H HR-MAS NMR and S180 cells: metabolite assignment and evaluation of pulse sequence
Oliveira,Aline L. de
HR-MAS NMR
sarcoma 180
S180
CPMG
title_short 1H HR-MAS NMR and S180 cells: metabolite assignment and evaluation of pulse sequence
title_full 1H HR-MAS NMR and S180 cells: metabolite assignment and evaluation of pulse sequence
title_fullStr 1H HR-MAS NMR and S180 cells: metabolite assignment and evaluation of pulse sequence
title_full_unstemmed 1H HR-MAS NMR and S180 cells: metabolite assignment and evaluation of pulse sequence
title_sort 1H HR-MAS NMR and S180 cells: metabolite assignment and evaluation of pulse sequence
author Oliveira,Aline L. de
author_facet Oliveira,Aline L. de
Martinelli,Bruno César B.
Lião,Luciano M.
Pereira,Flávia C.
Silveira-Lacerda,Elisangela P.
Alcantara,Glaucia B.
author_role author
author2 Martinelli,Bruno César B.
Lião,Luciano M.
Pereira,Flávia C.
Silveira-Lacerda,Elisangela P.
Alcantara,Glaucia B.
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Oliveira,Aline L. de
Martinelli,Bruno César B.
Lião,Luciano M.
Pereira,Flávia C.
Silveira-Lacerda,Elisangela P.
Alcantara,Glaucia B.
dc.subject.por.fl_str_mv HR-MAS NMR
sarcoma 180
S180
CPMG
topic HR-MAS NMR
sarcoma 180
S180
CPMG
description High resolution magic angle spinning ¹H nuclear magnetic resonance spectroscopy (HR-MAS NMR) is a useful technique for evaluation of intact cells and tissues. However, optimal NMR parameters are crucial in obtaining reliable results. To identify the key steps for the optimization of HR-MAS NMR parameters, we assessed different pulse sequences and NMR parameters using sarcoma 180 (S180) cells. A complete assignment of the metabolites of S180 is given to assist future studies.
publishDate 2014
dc.date.none.fl_str_mv 2014-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532014000600019
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532014000600019
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.5935/0103-5053.20140070
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Journal of the Brazilian Chemical Society v.25 n.6 2014
reponame:Journal of the Brazilian Chemical Society (Online)
instname:Sociedade Brasileira de Química (SBQ)
instacron:SBQ
instname_str Sociedade Brasileira de Química (SBQ)
instacron_str SBQ
institution SBQ
reponame_str Journal of the Brazilian Chemical Society (Online)
collection Journal of the Brazilian Chemical Society (Online)
repository.name.fl_str_mv Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)
repository.mail.fl_str_mv ||office@jbcs.sbq.org.br
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